Arginine-modified DNA Aptamers That Show Enantioselective Recognition of the Dicarboxylic Acid Moiety of Glutamic Acid
We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and t...
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| Veröffentlicht in: | Analytical Sciences 2008, Vol.24(1), pp.167-172 |
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| container_title | Analytical Sciences |
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| creator | OHSAWA, Kazuomi KASAMATSU, Toshiyuki NAGASHIMA, Jun-ichi HANAWA, Kazuo KUWAHARA, Masayasu OZAKI, Hiroaki SAWAI, Hiroaki |
| description | We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D -isomer from that of the L -isomer; however, the third aptamer did not show enantioselectivity. |
| doi_str_mv | 10.2116/analsci.24.167 |
| format | Article |
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Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D -isomer from that of the L -isomer; however, the third aptamer did not show enantioselectivity.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.24.167</identifier><identifier>PMID: 18187867</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Aptamers, Nucleotide - chemistry ; Arginine - chemistry ; Chemistry ; Chromatography, Affinity ; Dicarboxylic Acids - chemistry ; Glutamates - chemistry ; Magnetic Resonance Spectroscopy ; Reverse Transcriptase Polymerase Chain Reaction ; Spectrophotometry, Ultraviolet ; Stereoisomerism ; Surface Plasmon Resonance</subject><ispartof>Analytical Sciences, 2008, Vol.24(1), pp.167-172</ispartof><rights>2008 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2008</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-a237ab8998d5fba6cf1ae0c82a8583037de71176b44174ab1f009eb21563e7643</citedby><cites>FETCH-LOGICAL-c518t-a237ab8998d5fba6cf1ae0c82a8583037de71176b44174ab1f009eb21563e7643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.24.167$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.24.167$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,1879,4012,27906,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18187867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OHSAWA, Kazuomi</creatorcontrib><creatorcontrib>KASAMATSU, Toshiyuki</creatorcontrib><creatorcontrib>NAGASHIMA, Jun-ichi</creatorcontrib><creatorcontrib>HANAWA, Kazuo</creatorcontrib><creatorcontrib>KUWAHARA, Masayasu</creatorcontrib><creatorcontrib>OZAKI, Hiroaki</creatorcontrib><creatorcontrib>SAWAI, Hiroaki</creatorcontrib><title>Arginine-modified DNA Aptamers That Show Enantioselective Recognition of the Dicarboxylic Acid Moiety of Glutamic Acid</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D -isomer from that of the L -isomer; however, the third aptamer did not show enantioselectivity.</description><subject>Analytical Chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Arginine - chemistry</subject><subject>Chemistry</subject><subject>Chromatography, Affinity</subject><subject>Dicarboxylic Acids - chemistry</subject><subject>Glutamates - chemistry</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Stereoisomerism</subject><subject>Surface Plasmon Resonance</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2L1DAUxYso7rj66qMEBN86m68m6WOZ_VBYFXR9Lml6O5OhTcakXXf-ezO0rB8gvtwL9_zOSeBk2WuC15QQcaGd7qOxa8rXRMgn2YowrnJKuXiarXBJcC4Yx2fZixj3GBOqKH2enRFFlFRCrrL7Kmytsw7ywbe2s9Ciy08Vqg6jHiBEdLfTI_q68z_QldNutD5CD2a094C-gPFbZ9PNId-hcQfo0hodGv9w7K1BlbEt-ugtjMeTftNPKXO5v8yedenj8GrZ59m366u7zfv89vPNh011m5uCqDHXlEndqLJUbdE1WpiOaMBGUa0KxTCTLUhCpGg4J5LrhnQYl9BQUggGUnB2nr2bcw_Bf58gjvVgo4G-1w78FGuJKcZMFf8FSSkJLjlL4Nu_wL2fwqmFmnCuCiHLskzUeqZM8DEG6OpDsIMOx5rg-lRcvRRXU16n4pLhzRI7NQO0v_ClqQRczEBMkttC-O3df0VuZsc-jnoLj5E6jNb08Ac-j-R6VM1Ohxoc-wmaP71z</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>OHSAWA, Kazuomi</creator><creator>KASAMATSU, Toshiyuki</creator><creator>NAGASHIMA, Jun-ichi</creator><creator>HANAWA, Kazuo</creator><creator>KUWAHARA, Masayasu</creator><creator>OZAKI, Hiroaki</creator><creator>SAWAI, Hiroaki</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Arginine-modified DNA Aptamers That Show Enantioselective Recognition of the Dicarboxylic Acid Moiety of Glutamic Acid</title><author>OHSAWA, Kazuomi ; 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| source | J-STAGE Free; MEDLINE; Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Aptamers, Nucleotide - chemistry Arginine - chemistry Chemistry Chromatography, Affinity Dicarboxylic Acids - chemistry Glutamates - chemistry Magnetic Resonance Spectroscopy Reverse Transcriptase Polymerase Chain Reaction Spectrophotometry, Ultraviolet Stereoisomerism Surface Plasmon Resonance |
| title | Arginine-modified DNA Aptamers That Show Enantioselective Recognition of the Dicarboxylic Acid Moiety of Glutamic Acid |
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