CD86 molecule is a specific marker for canine monocyte-derived dendritic cells
In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (F...
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| Veröffentlicht in: | Veterinary Immunology and Immunopathology 2006-01, Vol.109 (1), p.167-176 |
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| container_title | Veterinary Immunology and Immunopathology |
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| creator | Bonnefont-Rebeix, Catherine de Carvalho, Camila Miranda Bernaud, Janine Chabanne, Luc Marchal, Thierry Rigal, Dominique |
| description | In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC. |
| doi_str_mv | 10.1016/j.vetimm.2005.08.027 |
| format | Article |
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We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.vetimm.2005.08.027</identifier><identifier>PMID: 16202456</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; B7-2 Antigen - immunology ; Biomarkers ; Canine monocyte-derived dendritic cells ; CD86 ; Cell Proliferation ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Dog ; Dogs - immunology ; Female ; Flow Cytometry - veterinary ; Granulocyte-Macrophage Colony-Stimulating Factor - immunology ; Histocompatibility Antigens Class II - immunology ; Interleukin-4 - immunology ; Kinetics ; Lymphocyte Activation - immunology ; Lymphocyte Culture Test, Mixed - veterinary ; Male ; Monocytes - cytology ; Monocytes - immunology</subject><ispartof>Veterinary Immunology and Immunopathology, 2006-01, Vol.109 (1), p.167-176</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-6242b2200866610dfddd49a93b54290486425a766b85389b5226b0712eebaed53</citedby><cites>FETCH-LOGICAL-c457t-6242b2200866610dfddd49a93b54290486425a766b85389b5226b0712eebaed53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2005.08.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16202456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bonnefont-Rebeix, Catherine</creatorcontrib><creatorcontrib>de Carvalho, Camila Miranda</creatorcontrib><creatorcontrib>Bernaud, Janine</creatorcontrib><creatorcontrib>Chabanne, Luc</creatorcontrib><creatorcontrib>Marchal, Thierry</creatorcontrib><creatorcontrib>Rigal, Dominique</creatorcontrib><title>CD86 molecule is a specific marker for canine monocyte-derived dendritic cells</title><title>Veterinary Immunology and Immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC.</description><subject>Animals</subject><subject>B7-2 Antigen - immunology</subject><subject>Biomarkers</subject><subject>Canine monocyte-derived dendritic cells</subject><subject>CD86</subject><subject>Cell Proliferation</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Dog</subject><subject>Dogs - immunology</subject><subject>Female</subject><subject>Flow Cytometry - veterinary</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</subject><subject>Histocompatibility Antigens Class II - immunology</subject><subject>Interleukin-4 - immunology</subject><subject>Kinetics</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Culture Test, Mixed - veterinary</subject><subject>Male</subject><subject>Monocytes - cytology</subject><subject>Monocytes - immunology</subject><issn>0165-2427</issn><issn>1873-2534</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAUhYMoOo7-A5Gu3LUmaZImG0HGJwy60XVok1vI2MeYtAPz703pgDtd3c13zj18CF0RnBFMxO0m28Hg2jajGPMMywzT4ggtiCzylPKcHaNFxHhKGS3O0HkIGxxBJeUpOiOCYsq4WKC31YMUSds3YMYGEheSMglbMK52JmlL_wU-qXufmLJzHUSw681-gNSCdzuwiYXOejdE2EDThAt0UpdNgMvDXaLPp8eP1Uu6fn9-Xd2vU8N4MaQijqpoHC6FEATb2lrLVKnyijOqMJOCUV4WQlSS51JVnFJR4YJQgKoEy_Mlupl7t77_HiEMunVhWlB20I9BF5gogYX6FyQF40rxCWQzaHwfgodab72LAvaaYD0J1xs9C9eTcI2ljsJj7PrQP1Yt2N_QwXAE7mYAoo6dA6-DcdAZsM6DGbTt3d8ffgCy3ZHc</recordid><startdate>20060115</startdate><enddate>20060115</enddate><creator>Bonnefont-Rebeix, Catherine</creator><creator>de Carvalho, Camila Miranda</creator><creator>Bernaud, Janine</creator><creator>Chabanne, Luc</creator><creator>Marchal, Thierry</creator><creator>Rigal, Dominique</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20060115</creationdate><title>CD86 molecule is a specific marker for canine monocyte-derived dendritic cells</title><author>Bonnefont-Rebeix, Catherine ; de Carvalho, Camila Miranda ; Bernaud, Janine ; Chabanne, Luc ; Marchal, Thierry ; Rigal, Dominique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-6242b2200866610dfddd49a93b54290486425a766b85389b5226b0712eebaed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>B7-2 Antigen - immunology</topic><topic>Biomarkers</topic><topic>Canine monocyte-derived dendritic cells</topic><topic>CD86</topic><topic>Cell Proliferation</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Dog</topic><topic>Dogs - immunology</topic><topic>Female</topic><topic>Flow Cytometry - veterinary</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</topic><topic>Histocompatibility Antigens Class II - immunology</topic><topic>Interleukin-4 - immunology</topic><topic>Kinetics</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Culture Test, Mixed - veterinary</topic><topic>Male</topic><topic>Monocytes - cytology</topic><topic>Monocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonnefont-Rebeix, Catherine</creatorcontrib><creatorcontrib>de Carvalho, Camila Miranda</creatorcontrib><creatorcontrib>Bernaud, Janine</creatorcontrib><creatorcontrib>Chabanne, Luc</creatorcontrib><creatorcontrib>Marchal, Thierry</creatorcontrib><creatorcontrib>Rigal, Dominique</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary Immunology and Immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonnefont-Rebeix, Catherine</au><au>de Carvalho, Camila Miranda</au><au>Bernaud, Janine</au><au>Chabanne, Luc</au><au>Marchal, Thierry</au><au>Rigal, Dominique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD86 molecule is a specific marker for canine monocyte-derived dendritic cells</atitle><jtitle>Veterinary Immunology and Immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2006-01-15</date><risdate>2006</risdate><volume>109</volume><issue>1</issue><spage>167</spage><epage>176</epage><pages>167-176</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><eissn>1365-2567</eissn><abstract>In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16202456</pmid><doi>10.1016/j.vetimm.2005.08.027</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals B7-2 Antigen - immunology Biomarkers Canine monocyte-derived dendritic cells CD86 Cell Proliferation Dendritic Cells - cytology Dendritic Cells - immunology Dog Dogs - immunology Female Flow Cytometry - veterinary Granulocyte-Macrophage Colony-Stimulating Factor - immunology Histocompatibility Antigens Class II - immunology Interleukin-4 - immunology Kinetics Lymphocyte Activation - immunology Lymphocyte Culture Test, Mixed - veterinary Male Monocytes - cytology Monocytes - immunology |
| title | CD86 molecule is a specific marker for canine monocyte-derived dendritic cells |
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