Improved 2-nitrobenzenesulfenyl method: optimization of the protocol and improved enrichment for labeled peptides
We have developed the NBS (2‐nitrobenzenesulfenyl) method, a quantitative proteome analysis method utilizing stable isotope labeling followed by mass spectrometry. The potential of this method was reported previously, and the procedure has now been further optimized. Here, we describe a procedure ut...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2006-01, Vol.20 (1), p.31-38 |
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| container_title | Rapid communications in mass spectrometry |
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| creator | Matsuo, Ei-ichi Toda, Chikako Watanabe, Makoto Iida, Tetsuo Masuda, Taro Minohata, Toshikazu Ando, Eiji Tsunasawa, Susumu Nishimura, Osamu |
| description | We have developed the NBS (2‐nitrobenzenesulfenyl) method, a quantitative proteome analysis method utilizing stable isotope labeling followed by mass spectrometry. The potential of this method was reported previously, and the procedure has now been further optimized. Here, we describe a procedure utilizing urea or guanidine hydrochloride as a protein denaturant, in conjunction with an improved chromatographic enrichment method for the NBS‐labeled peptides using a phenyl resin column. By using this new protocol, both sample loss throughout the protocol and the elution of unwanted unlabeled peptides can be minimized, improving the efficiency of the analysis significantly. Copyright © 2005 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/rcm.2262 |
| format | Article |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Chickens Guanidine Mice Nitrobenzenes - chemistry Peptides - analysis Peptides - chemistry Protein Denaturation Proteomics - methods Sequence Analysis, Protein Urea |
| title | Improved 2-nitrobenzenesulfenyl method: optimization of the protocol and improved enrichment for labeled peptides |
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