Recombineering linear DNA that replicate stably in E. coli

The advent of recombineering technology in Escherichia coli has revolutionized the way recombinant DNA molecules are constructed. We present a novel application of recombineering to linearize DNA by capping their ends with individual telomeres derived from bacteriophage N15, which exists as a linear...

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Veröffentlicht in:	Plasmid 2008, Vol.59 (1), p.63-71
Hauptverfasser:	Ooi, Yaw-Shin, Warburton, Peter E., Ravin, Nikolai V., Narayanan, Kumaran
Format:	Artikel
Sprache:	eng
Schlagworte:	BACs Chromosomes, Artificial, Bacterial - genetics Coliphages - genetics DNA Replication - genetics DNA, Recombinant - biosynthesis Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Genetic Engineering Humans Linear Phage N15 Plasmids - genetics RecBCD Recombineering Telomere - genetics Telomeres β-Globin
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