A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica
Multifunctional nanocomposites (M‐CLEAs) of enzymes and magnetic nanoparticles (M‐NPs) were fabricated in hierarchically ordered, mesocellular, mesoporous silica (HMMS; see Figure) by a simple process involving the co‐adsorption of enzyme molecules and magnetic nanoparticles into HMMS followed by gl...
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| Veröffentlicht in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2005-12, Vol.1 (12), p.1203-1207 |
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| creator | Kim, Jungbae Lee, Jinwoo Na, Hyon Bin Kim, Byoung Chan Youn, Jong Kyu Kwak, Ja Hun Moon, Karam Lee, Eunwoong Kim, Jaeyun Park, Jongnam Dohnalkova, Alice Park, Hyun Gyu Gu, Man Bock Chang, Ho Nam Grate, Jay W. Hyeon, Taeghwan |
| description | Multifunctional nanocomposites (M‐CLEAs) of enzymes and magnetic nanoparticles (M‐NPs) were fabricated in hierarchically ordered, mesocellular, mesoporous silica (HMMS; see Figure) by a simple process involving the co‐adsorption of enzyme molecules and magnetic nanoparticles into HMMS followed by glutaraldehyde (GA) treatment. These nanocomposites are magnetically separable and highly stable and active. In particular, M‐CLEA–lipase shows no decrease of lipase activity at all in the presence of proteases. |
| doi_str_mv | 10.1002/smll.200500245 |
| format | Article |
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These nanocomposites are magnetically separable and highly stable and active. In particular, M‐CLEA–lipase shows no decrease of lipase activity at all in the presence of proteases.</description><identifier>ISSN: 1613-6810</identifier><identifier>EISSN: 1613-6829</identifier><identifier>DOI: 10.1002/smll.200500245</identifier><identifier>PMID: 17193420</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>BASIC BIOLOGICAL SCIENCES ; Catalysis ; Crystallization ; Environmental Molecular Sciences Laboratory ; enzyme catalysis ; enzyme stabilization ; Enzymes - chemistry ; IMMOBILIZED ENZYMES ; IRON OXIDES ; LIFETIME ; magnetic nanoparticles ; magnetic separation ; MAGNETIC SEPARATORS ; Magnetics ; mesoporous materials ; mesoporous silica ; Microscopy, Electron, Transmission ; nanobiotechnology ; Nanocomposites - chemistry ; Nanoparticles - chemistry ; nanoscale enzyme reactors ; NANOSTRUCTURES ; Nanotubes ; Peptide Hydrolases - chemistry ; POROSITY ; SEPARATION PROCESSES ; SILICA ; Silicon Dioxide - chemistry ; STABILITY ; SURFACE AREA</subject><ispartof>Small (Weinheim an der Bergstrasse, Germany), 2005-12, Vol.1 (12), p.1203-1207</ispartof><rights>Copyright © 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4735-f3bf84ca845700c8aeabb042347c2511dd1a4cdfd9273218b0dc037e068435293</citedby><cites>FETCH-LOGICAL-c4735-f3bf84ca845700c8aeabb042347c2511dd1a4cdfd9273218b0dc037e068435293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsmll.200500245$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsmll.200500245$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17193420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/876931$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jungbae</creatorcontrib><creatorcontrib>Lee, Jinwoo</creatorcontrib><creatorcontrib>Na, Hyon Bin</creatorcontrib><creatorcontrib>Kim, Byoung Chan</creatorcontrib><creatorcontrib>Youn, Jong Kyu</creatorcontrib><creatorcontrib>Kwak, Ja Hun</creatorcontrib><creatorcontrib>Moon, Karam</creatorcontrib><creatorcontrib>Lee, Eunwoong</creatorcontrib><creatorcontrib>Kim, Jaeyun</creatorcontrib><creatorcontrib>Park, Jongnam</creatorcontrib><creatorcontrib>Dohnalkova, Alice</creatorcontrib><creatorcontrib>Park, Hyun Gyu</creatorcontrib><creatorcontrib>Gu, Man Bock</creatorcontrib><creatorcontrib>Chang, Ho Nam</creatorcontrib><creatorcontrib>Grate, Jay W.</creatorcontrib><creatorcontrib>Hyeon, Taeghwan</creatorcontrib><creatorcontrib>Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)</creatorcontrib><title>A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica</title><title>Small (Weinheim an der Bergstrasse, Germany)</title><addtitle>Small</addtitle><description>Multifunctional nanocomposites (M‐CLEAs) of enzymes and magnetic nanoparticles (M‐NPs) were fabricated in hierarchically ordered, mesocellular, mesoporous silica (HMMS; see Figure) by a simple process involving the co‐adsorption of enzyme molecules and magnetic nanoparticles into HMMS followed by glutaraldehyde (GA) treatment. These nanocomposites are magnetically separable and highly stable and active. In particular, M‐CLEA–lipase shows no decrease of lipase activity at all in the presence of proteases.</description><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Catalysis</subject><subject>Crystallization</subject><subject>Environmental Molecular Sciences Laboratory</subject><subject>enzyme catalysis</subject><subject>enzyme stabilization</subject><subject>Enzymes - chemistry</subject><subject>IMMOBILIZED ENZYMES</subject><subject>IRON OXIDES</subject><subject>LIFETIME</subject><subject>magnetic nanoparticles</subject><subject>magnetic separation</subject><subject>MAGNETIC SEPARATORS</subject><subject>Magnetics</subject><subject>mesoporous materials</subject><subject>mesoporous silica</subject><subject>Microscopy, Electron, Transmission</subject><subject>nanobiotechnology</subject><subject>Nanocomposites - chemistry</subject><subject>Nanoparticles - chemistry</subject><subject>nanoscale enzyme reactors</subject><subject>NANOSTRUCTURES</subject><subject>Nanotubes</subject><subject>Peptide Hydrolases - chemistry</subject><subject>POROSITY</subject><subject>SEPARATION PROCESSES</subject><subject>SILICA</subject><subject>Silicon Dioxide - chemistry</subject><subject>STABILITY</subject><subject>SURFACE AREA</subject><issn>1613-6810</issn><issn>1613-6829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhSNERUthyxKZDavO4FfiZFmq0lbKtELhIXVjOc5Nx-DEwc4Iwg_j9-Eh0cCOle-1v3PutU6SvCB4TTCmb0Jn7ZpinMaGp4-SE5IRtspyWjw-1AQfJ09D-IIxI5SLJ8kxEaRgnOKT5Nc52qiHHkajlbUTqmBQXtUWztC1edjub8Z9iy77n1MHqJrCCB16qwI0yPXoVvVOu25wwYwQkGsXMCDVNwfrP1g0jqWNT9XWDEPUmz4OAa-83i7j73wDHpoztIHgNFi7s8rP3eC820WtsZF9lhy1ygZ4vpynycd3lx8urlfl3dXNxXm50lywdNWyus25VjlPBcY6V6DqGnPKuNA0JaRpiOK6aZuCCkZJXuNGYyYAZzlnKS3YafJq9nVhNDLo-Em91a7vQY8yF1nBSGRez8zg3bcdhFF2Jux3Vz3EjaXAJKcs5RFcz6D2LgQPrRy86ZSfJMFyn6bcpykPaUbBy8V5V3fQ_MWX-CJQzMB3Y2H6j52sNmX5r_lq1pqY6I-DVvmvMhNMpPLz7ZUs6X11v3lfyk_sN-Jlvkw</recordid><startdate>200512</startdate><enddate>200512</enddate><creator>Kim, Jungbae</creator><creator>Lee, Jinwoo</creator><creator>Na, Hyon Bin</creator><creator>Kim, Byoung Chan</creator><creator>Youn, Jong Kyu</creator><creator>Kwak, Ja Hun</creator><creator>Moon, Karam</creator><creator>Lee, Eunwoong</creator><creator>Kim, Jaeyun</creator><creator>Park, Jongnam</creator><creator>Dohnalkova, Alice</creator><creator>Park, Hyun Gyu</creator><creator>Gu, Man Bock</creator><creator>Chang, Ho Nam</creator><creator>Grate, Jay W.</creator><creator>Hyeon, Taeghwan</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>200512</creationdate><title>A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica</title><author>Kim, Jungbae ; Lee, Jinwoo ; Na, Hyon Bin ; Kim, Byoung Chan ; Youn, Jong Kyu ; Kwak, Ja Hun ; Moon, Karam ; Lee, Eunwoong ; Kim, Jaeyun ; Park, Jongnam ; Dohnalkova, Alice ; Park, Hyun Gyu ; Gu, Man Bock ; Chang, Ho Nam ; Grate, Jay W. ; Hyeon, Taeghwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4735-f3bf84ca845700c8aeabb042347c2511dd1a4cdfd9273218b0dc037e068435293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Catalysis</topic><topic>Crystallization</topic><topic>Environmental Molecular Sciences Laboratory</topic><topic>enzyme catalysis</topic><topic>enzyme stabilization</topic><topic>Enzymes - chemistry</topic><topic>IMMOBILIZED ENZYMES</topic><topic>IRON OXIDES</topic><topic>LIFETIME</topic><topic>magnetic nanoparticles</topic><topic>magnetic separation</topic><topic>MAGNETIC SEPARATORS</topic><topic>Magnetics</topic><topic>mesoporous materials</topic><topic>mesoporous silica</topic><topic>Microscopy, Electron, Transmission</topic><topic>nanobiotechnology</topic><topic>Nanocomposites - chemistry</topic><topic>Nanoparticles - chemistry</topic><topic>nanoscale enzyme reactors</topic><topic>NANOSTRUCTURES</topic><topic>Nanotubes</topic><topic>Peptide Hydrolases - chemistry</topic><topic>POROSITY</topic><topic>SEPARATION PROCESSES</topic><topic>SILICA</topic><topic>Silicon Dioxide - chemistry</topic><topic>STABILITY</topic><topic>SURFACE AREA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jungbae</creatorcontrib><creatorcontrib>Lee, Jinwoo</creatorcontrib><creatorcontrib>Na, Hyon Bin</creatorcontrib><creatorcontrib>Kim, Byoung Chan</creatorcontrib><creatorcontrib>Youn, Jong Kyu</creatorcontrib><creatorcontrib>Kwak, Ja Hun</creatorcontrib><creatorcontrib>Moon, Karam</creatorcontrib><creatorcontrib>Lee, Eunwoong</creatorcontrib><creatorcontrib>Kim, Jaeyun</creatorcontrib><creatorcontrib>Park, Jongnam</creatorcontrib><creatorcontrib>Dohnalkova, Alice</creatorcontrib><creatorcontrib>Park, Hyun Gyu</creatorcontrib><creatorcontrib>Gu, Man Bock</creatorcontrib><creatorcontrib>Chang, Ho Nam</creatorcontrib><creatorcontrib>Grate, Jay W.</creatorcontrib><creatorcontrib>Hyeon, Taeghwan</creatorcontrib><creatorcontrib>Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jungbae</au><au>Lee, Jinwoo</au><au>Na, Hyon Bin</au><au>Kim, Byoung Chan</au><au>Youn, Jong Kyu</au><au>Kwak, Ja Hun</au><au>Moon, Karam</au><au>Lee, Eunwoong</au><au>Kim, Jaeyun</au><au>Park, Jongnam</au><au>Dohnalkova, Alice</au><au>Park, Hyun Gyu</au><au>Gu, Man Bock</au><au>Chang, Ho Nam</au><au>Grate, Jay W.</au><au>Hyeon, Taeghwan</au><aucorp>Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica</atitle><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle><addtitle>Small</addtitle><date>2005-12</date><risdate>2005</risdate><volume>1</volume><issue>12</issue><spage>1203</spage><epage>1207</epage><pages>1203-1207</pages><issn>1613-6810</issn><eissn>1613-6829</eissn><abstract>Multifunctional nanocomposites (M‐CLEAs) of enzymes and magnetic nanoparticles (M‐NPs) were fabricated in hierarchically ordered, mesocellular, mesoporous silica (HMMS; see Figure) by a simple process involving the co‐adsorption of enzyme molecules and magnetic nanoparticles into HMMS followed by glutaraldehyde (GA) treatment. These nanocomposites are magnetically separable and highly stable and active. In particular, M‐CLEA–lipase shows no decrease of lipase activity at all in the presence of proteases.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>17193420</pmid><doi>10.1002/smll.200500245</doi><tpages>5</tpages></addata></record> |
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| subjects | BASIC BIOLOGICAL SCIENCES Catalysis Crystallization Environmental Molecular Sciences Laboratory enzyme catalysis enzyme stabilization Enzymes - chemistry IMMOBILIZED ENZYMES IRON OXIDES LIFETIME magnetic nanoparticles magnetic separation MAGNETIC SEPARATORS Magnetics mesoporous materials mesoporous silica Microscopy, Electron, Transmission nanobiotechnology Nanocomposites - chemistry Nanoparticles - chemistry nanoscale enzyme reactors NANOSTRUCTURES Nanotubes Peptide Hydrolases - chemistry POROSITY SEPARATION PROCESSES SILICA Silicon Dioxide - chemistry STABILITY SURFACE AREA |
| title | A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica |
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