Label-Free DNA Detection of Hepatitis C Virus Based on Modified Conducting Polypyrrole Films at Microelectrodes and Atomic Force Microscopy Tip-Integrated Electrodes

We present a new strategy for the label-free electrochemical detection of DNA hybridization for detecting hepatitis C virus based on electrostatic modulation of the ion-exchange kinetics of a polypyrrole film deposited at microelectrodes. Synthetic single-stranded 18-mer HCV genotype-1-specific prob...

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Veröffentlicht in:	Analytical chemistry (Washington) 2008-01, Vol.80 (1), p.237-245
Hauptverfasser:	Riccardi, Carla dos Santos, Kranz, Christine, Kowalik, Janusz, Yamanaka, Hideko, Mizaikoff, Boris, Josowicz, Mira
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Base Sequence Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Chemical reactions Chemistry Deoxyribonucleic acid DNA DNA Probes - analysis DNA Probes - chemistry Electrochemical methods Electrodes Exact sciences and technology Fundamental and applied biological sciences. Psychology Hepacivirus - genetics Hepacivirus - isolation & purification Hepatitis C Hepatitis C virus Humans Ion Exchange Membranes, Artificial Methods. Procedures. Technologies Microelectrodes Microscopy, Atomic Force Nucleic Acid Hybridization Polycarboxylate Cement - chemistry Polymer films Polymers - chemistry Porosity Pyrroles - chemistry Reproducibility of Results Sensitivity and Specificity Studies Various methods and equipments
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