Stoichiometry-Dependent Formation of Quantum Dot−Antibody Bioconjugates: A Complementary Atomic Force Microscopy and Agarose Gel Electrophoresis Study
The unique advantages of quantum dot (QD) bioconjugates have motivated their application in biological assays. However, physical characterization of bioconjugated QDs after surface modification has often been overlooked. Here, biotinylated antibodies (biotin−IgG) were attached to commercial streptav...
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| Veröffentlicht in: | The journal of physical chemistry. B 2005-11, Vol.109 (44), p.20724-20730 |
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| container_title | The journal of physical chemistry. B |
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| creator | Nehilla, Barrett J Vu, Tania Q Desai, Tejal A |
| description | The unique advantages of quantum dot (QD) bioconjugates have motivated their application in biological assays. However, physical characterization of bioconjugated QDs after surface modification has often been overlooked. Here, biotinylated antibodies (biotin−IgG) were attached to commercial streptavidin-conjugated quantum dots (strep−QDs) at different stoichiometric ratios, and these QD bioconjugates were characterized with atomic force microscopy and discontinuous sodium dodecyl sulfate agarose gel electrophoresis (SDS-AGE). The results from these complementary analytical techniques showed that the molar ratio determined the relative sizes, molecular weights and morphologies of the QD bioconjugates. Additionally, the novel discontinuous SDS-AGE analysis confirmed specific binding between biotin−IgG and strep−QDs. Researchers who design QD bioconjugates for cell-based assays should consider stoichiometry-dependent differences in the physical properties of their QD bioconjugates. |
| doi_str_mv | 10.1021/jp052613+ |
| format | Article |
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Researchers who design QD bioconjugates for cell-based assays should consider stoichiometry-dependent differences in the physical properties of their QD bioconjugates.</description><subject>Antibodies - chemistry</subject><subject>Biotin</subject><subject>Electrophoresis, Agar Gel</subject><subject>Microscopy, Atomic Force</subject><subject>Protein Binding</subject><subject>Quantum Dots</subject><subject>Streptavidin</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkU1v1DAQhiMEoqVw4A8gHxBCQgF_xPngFnb7gVREq10kbpbjTFovsR1sRyI3jvTaK_-uv6RZ7QIHDqOZ0Tx6R-9Mkjwn-C3BlLzbDJjTnLA3D5JDwilO5yge7uuc4PwgeRLCBmPKaZk_Tg5IXnKWl_lh8nsVnVbX2hmIfkqXMIBtwUZ04ryRUTuLXIcuR2njaNDSxbtft7WNunHthD5op5zdjFcyQnh_9_MG1WjhzNCDmSWkn1AdndFqK6YAfdLKu6DcMCFpW1RfybkFdAo9Ou5BRe-Ga-ch6IBWcWynp8mjTvYBnu3zUfLl5Hi9OEvPP59-XNTnqWRZGVMmM1K0rCGMZVVTVUzSUlGiOFeqaStcctoUuFPQKIrzhlek6njRZG1ZctkqzI6SVzvdwbvvI4QojA4K-l5acGMQBSYF5byawdc7cOsjeOjE4LWZfQqCxfYT4s8nZvTFXnNsDLT_wP3lZyDdATpE-PF3Lv03kRes4GJ9sRIX66_LrLokYsu_3PFSBbFxo7fzSf7few_UkqKk</recordid><startdate>20051110</startdate><enddate>20051110</enddate><creator>Nehilla, Barrett J</creator><creator>Vu, Tania Q</creator><creator>Desai, Tejal A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051110</creationdate><title>Stoichiometry-Dependent Formation of Quantum Dot−Antibody Bioconjugates: A Complementary Atomic Force Microscopy and Agarose Gel Electrophoresis Study</title><author>Nehilla, Barrett J ; Vu, Tania Q ; Desai, Tejal A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a348t-3a417d3b13349b993a28c21c55ccbd90852b70fcebc206b5919f57b4d885adc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antibodies - chemistry</topic><topic>Biotin</topic><topic>Electrophoresis, Agar Gel</topic><topic>Microscopy, Atomic Force</topic><topic>Protein Binding</topic><topic>Quantum Dots</topic><topic>Streptavidin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nehilla, Barrett J</creatorcontrib><creatorcontrib>Vu, Tania Q</creatorcontrib><creatorcontrib>Desai, Tejal A</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of physical chemistry. 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| ispartof | The journal of physical chemistry. B, 2005-11, Vol.109 (44), p.20724-20730 |
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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Antibodies - chemistry Biotin Electrophoresis, Agar Gel Microscopy, Atomic Force Protein Binding Quantum Dots Streptavidin |
| title | Stoichiometry-Dependent Formation of Quantum Dot−Antibody Bioconjugates: A Complementary Atomic Force Microscopy and Agarose Gel Electrophoresis Study |
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