Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures
To investigate the effects of corneal fibroblasts on the differentiation of corneal epithelial cells in a coculture system based on a collagen vitrigel membrane. Simian virus 40-transformed human corneal epithelial (HCE) cells and human corneal fibroblasts were cultured on opposite sides of a collag...
Gespeichert in:
| Veröffentlicht in: | Investigative ophthalmology & visual science 2008, Vol.49 (1), p.113-119 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 119 |
|---|---|
| container_issue | 1 |
| container_start_page | 113 |
| container_title | Investigative ophthalmology & visual science |
| container_volume | 49 |
| creator | KO, Ji-Ae YANG LIU YANAI, Ryoji CBIKALNA, Tai-Ichiro TAKEZAWA, Toshiaki NISBIDA, Teruo |
| description | To investigate the effects of corneal fibroblasts on the differentiation of corneal epithelial cells in a coculture system based on a collagen vitrigel membrane.
Simian virus 40-transformed human corneal epithelial (HCE) cells and human corneal fibroblasts were cultured on opposite sides of a collagen vitrigel membrane. The distribution of HCE cells and corneal fibroblasts on the collagen membrane was determined by immunofluorescence staining and immunoblot analysis of marker proteins. Expression of the tight-junctional proteins ZO-1, occludin, and claudin and of the adherens-junctional proteins E- and N-cadherin in HCE cells was determined at the mRNA and protein levels by reverse transcription-polymerase chain reaction analysis and immunoblot analysis, respectively.
The abundance of ZO-1, occludin, and claudin mRNA and proteins in HCE cells was markedly increased by coculture with corneal fibroblasts. The expression of E- or N-cadherin did not differ between HCE cells cultured with corneal fibroblasts and those cultured without them. PD98059, a specific inhibitor of signaling by extracellular signal regulated kinase (ERK), prevented the upregulation of tight-junctional proteins in HCE cells by corneal fibroblasts.
Human corneal fibroblasts regulated the expression of tight-junctional proteins in HCE cells, suggesting that corneal fibroblasts may play an important role in the differentiation of corneal epithelial cells. |
| doi_str_mv | 10.1167/iovs.07-0353 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70170421</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70170421</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-e93911384ff8af39749e814b7e5327df9605894919bc3c7170dbd95e5d30be5d3</originalsourceid><addsrcrecordid>eNpFkEFP3DAQha2qiN0Ct56rXNoTgZk4XtvHasXSIiQ4ANfISSa7rrzx1naQ4Nc30UblMjOa-d7T6DH2FeEKcSWvrX-NVyBz4IJ_YksUosiFVPwzWwKWqxxKKBfsS4x_AArEAk7ZAhXKAlSxZO_Ph0DbwZlkfZ_5Lnuy213K74a-mTbGZY_BJ7J9zGyfrX3oadzdHGzakbPjuCbnYla__b9tbB187UxMs8Q5s6U-e7Ep2C2NisGlIVA8ZyedcZEu5n7Gnjc3T-tf-f3D7e_1z_u84UqknDTXiFyVXadMx7UsNSksa0mCF7Lt9AqE0qVGXTe8kSihrVstSLQc6qmesR9H30PwfweKqdrb2Ixvm578ECsJo6YscAQvj2ATfIyBuuoQ7N6EtwqhmrKupqwrkNWU9Yh_m32Hek_tBzyHOwLfZ8DExrgumL6x8YPTWiFgwf8BIjGI0A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70170421</pqid></control><display><type>article</type><title>Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>KO, Ji-Ae ; YANG LIU ; YANAI, Ryoji ; CBIKALNA, Tai-Ichiro ; TAKEZAWA, Toshiaki ; NISBIDA, Teruo</creator><creatorcontrib>KO, Ji-Ae ; YANG LIU ; YANAI, Ryoji ; CBIKALNA, Tai-Ichiro ; TAKEZAWA, Toshiaki ; NISBIDA, Teruo</creatorcontrib><description>To investigate the effects of corneal fibroblasts on the differentiation of corneal epithelial cells in a coculture system based on a collagen vitrigel membrane.
Simian virus 40-transformed human corneal epithelial (HCE) cells and human corneal fibroblasts were cultured on opposite sides of a collagen vitrigel membrane. The distribution of HCE cells and corneal fibroblasts on the collagen membrane was determined by immunofluorescence staining and immunoblot analysis of marker proteins. Expression of the tight-junctional proteins ZO-1, occludin, and claudin and of the adherens-junctional proteins E- and N-cadherin in HCE cells was determined at the mRNA and protein levels by reverse transcription-polymerase chain reaction analysis and immunoblot analysis, respectively.
The abundance of ZO-1, occludin, and claudin mRNA and proteins in HCE cells was markedly increased by coculture with corneal fibroblasts. The expression of E- or N-cadherin did not differ between HCE cells cultured with corneal fibroblasts and those cultured without them. PD98059, a specific inhibitor of signaling by extracellular signal regulated kinase (ERK), prevented the upregulation of tight-junctional proteins in HCE cells by corneal fibroblasts.
Human corneal fibroblasts regulated the expression of tight-junctional proteins in HCE cells, suggesting that corneal fibroblasts may play an important role in the differentiation of corneal epithelial cells.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.07-0353</identifier><identifier>PMID: 18172082</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: Association for Research in Vision and Ophtalmology</publisher><subject>Antigens, CD - genetics ; Antigens, CD - metabolism ; Biological and medical sciences ; Cadherins - genetics ; Cadherins - metabolism ; Calcium-Calmodulin-Dependent Protein Kinases - pharmacology ; Cell Differentiation ; Cell Line, Transformed ; Claudin-1 ; Coculture Techniques ; Collagen ; Cornea - cytology ; Epithelium, Corneal - drug effects ; Epithelium, Corneal - metabolism ; Eye and associated structures. Visual pathways and centers. Vision ; Fibroblasts - physiology ; Flavonoids - pharmacology ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoblotting ; Medical sciences ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Occludin ; Ophthalmology ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Simian virus 40 ; Up-Regulation - physiology ; Vertebrates: nervous system and sense organs ; Zonula Occludens-1 Protein</subject><ispartof>Investigative ophthalmology & visual science, 2008, Vol.49 (1), p.113-119</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-e93911384ff8af39749e814b7e5327df9605894919bc3c7170dbd95e5d30be5d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19981012$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18172082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KO, Ji-Ae</creatorcontrib><creatorcontrib>YANG LIU</creatorcontrib><creatorcontrib>YANAI, Ryoji</creatorcontrib><creatorcontrib>CBIKALNA, Tai-Ichiro</creatorcontrib><creatorcontrib>TAKEZAWA, Toshiaki</creatorcontrib><creatorcontrib>NISBIDA, Teruo</creatorcontrib><title>Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To investigate the effects of corneal fibroblasts on the differentiation of corneal epithelial cells in a coculture system based on a collagen vitrigel membrane.
Simian virus 40-transformed human corneal epithelial (HCE) cells and human corneal fibroblasts were cultured on opposite sides of a collagen vitrigel membrane. The distribution of HCE cells and corneal fibroblasts on the collagen membrane was determined by immunofluorescence staining and immunoblot analysis of marker proteins. Expression of the tight-junctional proteins ZO-1, occludin, and claudin and of the adherens-junctional proteins E- and N-cadherin in HCE cells was determined at the mRNA and protein levels by reverse transcription-polymerase chain reaction analysis and immunoblot analysis, respectively.
The abundance of ZO-1, occludin, and claudin mRNA and proteins in HCE cells was markedly increased by coculture with corneal fibroblasts. The expression of E- or N-cadherin did not differ between HCE cells cultured with corneal fibroblasts and those cultured without them. PD98059, a specific inhibitor of signaling by extracellular signal regulated kinase (ERK), prevented the upregulation of tight-junctional proteins in HCE cells by corneal fibroblasts.
Human corneal fibroblasts regulated the expression of tight-junctional proteins in HCE cells, suggesting that corneal fibroblasts may play an important role in the differentiation of corneal epithelial cells.</description><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - pharmacology</subject><subject>Cell Differentiation</subject><subject>Cell Line, Transformed</subject><subject>Claudin-1</subject><subject>Coculture Techniques</subject><subject>Collagen</subject><subject>Cornea - cytology</subject><subject>Epithelium, Corneal - drug effects</subject><subject>Epithelium, Corneal - metabolism</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fibroblasts - physiology</subject><subject>Flavonoids - pharmacology</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Medical sciences</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Occludin</subject><subject>Ophthalmology</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Simian virus 40</subject><subject>Up-Regulation - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Zonula Occludens-1 Protein</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFP3DAQha2qiN0Ct56rXNoTgZk4XtvHasXSIiQ4ANfISSa7rrzx1naQ4Nc30UblMjOa-d7T6DH2FeEKcSWvrX-NVyBz4IJ_YksUosiFVPwzWwKWqxxKKBfsS4x_AArEAk7ZAhXKAlSxZO_Ph0DbwZlkfZ_5Lnuy213K74a-mTbGZY_BJ7J9zGyfrX3oadzdHGzakbPjuCbnYla__b9tbB187UxMs8Q5s6U-e7Ep2C2NisGlIVA8ZyedcZEu5n7Gnjc3T-tf-f3D7e_1z_u84UqknDTXiFyVXadMx7UsNSksa0mCF7Lt9AqE0qVGXTe8kSihrVstSLQc6qmesR9H30PwfweKqdrb2Ixvm578ECsJo6YscAQvj2ATfIyBuuoQ7N6EtwqhmrKupqwrkNWU9Yh_m32Hek_tBzyHOwLfZ8DExrgumL6x8YPTWiFgwf8BIjGI0A</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>KO, Ji-Ae</creator><creator>YANG LIU</creator><creator>YANAI, Ryoji</creator><creator>CBIKALNA, Tai-Ichiro</creator><creator>TAKEZAWA, Toshiaki</creator><creator>NISBIDA, Teruo</creator><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures</title><author>KO, Ji-Ae ; YANG LIU ; YANAI, Ryoji ; CBIKALNA, Tai-Ichiro ; TAKEZAWA, Toshiaki ; NISBIDA, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-e93911384ff8af39749e814b7e5327df9605894919bc3c7170dbd95e5d30be5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - pharmacology</topic><topic>Cell Differentiation</topic><topic>Cell Line, Transformed</topic><topic>Claudin-1</topic><topic>Coculture Techniques</topic><topic>Collagen</topic><topic>Cornea - cytology</topic><topic>Epithelium, Corneal - drug effects</topic><topic>Epithelium, Corneal - metabolism</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fibroblasts - physiology</topic><topic>Flavonoids - pharmacology</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Medical sciences</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Occludin</topic><topic>Ophthalmology</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Simian virus 40</topic><topic>Up-Regulation - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Zonula Occludens-1 Protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KO, Ji-Ae</creatorcontrib><creatorcontrib>YANG LIU</creatorcontrib><creatorcontrib>YANAI, Ryoji</creatorcontrib><creatorcontrib>CBIKALNA, Tai-Ichiro</creatorcontrib><creatorcontrib>TAKEZAWA, Toshiaki</creatorcontrib><creatorcontrib>NISBIDA, Teruo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KO, Ji-Ae</au><au>YANG LIU</au><au>YANAI, Ryoji</au><au>CBIKALNA, Tai-Ichiro</au><au>TAKEZAWA, Toshiaki</au><au>NISBIDA, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2008</date><risdate>2008</risdate><volume>49</volume><issue>1</issue><spage>113</spage><epage>119</epage><pages>113-119</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To investigate the effects of corneal fibroblasts on the differentiation of corneal epithelial cells in a coculture system based on a collagen vitrigel membrane.
Simian virus 40-transformed human corneal epithelial (HCE) cells and human corneal fibroblasts were cultured on opposite sides of a collagen vitrigel membrane. The distribution of HCE cells and corneal fibroblasts on the collagen membrane was determined by immunofluorescence staining and immunoblot analysis of marker proteins. Expression of the tight-junctional proteins ZO-1, occludin, and claudin and of the adherens-junctional proteins E- and N-cadherin in HCE cells was determined at the mRNA and protein levels by reverse transcription-polymerase chain reaction analysis and immunoblot analysis, respectively.
The abundance of ZO-1, occludin, and claudin mRNA and proteins in HCE cells was markedly increased by coculture with corneal fibroblasts. The expression of E- or N-cadherin did not differ between HCE cells cultured with corneal fibroblasts and those cultured without them. PD98059, a specific inhibitor of signaling by extracellular signal regulated kinase (ERK), prevented the upregulation of tight-junctional proteins in HCE cells by corneal fibroblasts.
Human corneal fibroblasts regulated the expression of tight-junctional proteins in HCE cells, suggesting that corneal fibroblasts may play an important role in the differentiation of corneal epithelial cells.</abstract><cop>Rockville, MD</cop><pub>Association for Research in Vision and Ophtalmology</pub><pmid>18172082</pmid><doi>10.1167/iovs.07-0353</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-0404 |
| ispartof | Investigative ophthalmology & visual science, 2008, Vol.49 (1), p.113-119 |
| issn | 0146-0404 1552-5783 1552-5783 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70170421 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antigens, CD - genetics Antigens, CD - metabolism Biological and medical sciences Cadherins - genetics Cadherins - metabolism Calcium-Calmodulin-Dependent Protein Kinases - pharmacology Cell Differentiation Cell Line, Transformed Claudin-1 Coculture Techniques Collagen Cornea - cytology Epithelium, Corneal - drug effects Epithelium, Corneal - metabolism Eye and associated structures. Visual pathways and centers. Vision Fibroblasts - physiology Flavonoids - pharmacology Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Humans Immunoblotting Medical sciences Membrane Proteins - genetics Membrane Proteins - metabolism Occludin Ophthalmology Phosphoproteins - genetics Phosphoproteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Simian virus 40 Up-Regulation - physiology Vertebrates: nervous system and sense organs Zonula Occludens-1 Protein |
| title | Upregulation of Tight-Junctional Proteins in Corneal Epithelial Cells by Corneal Fibroblasts in Collagen Vitrigel Cultures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T15%3A41%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Upregulation%20of%20Tight-Junctional%20Proteins%20in%20Corneal%20Epithelial%20Cells%20by%20Corneal%20Fibroblasts%20in%20Collagen%20Vitrigel%20Cultures&rft.jtitle=Investigative%20ophthalmology%20&%20visual%20science&rft.au=KO,%20Ji-Ae&rft.date=2008&rft.volume=49&rft.issue=1&rft.spage=113&rft.epage=119&rft.pages=113-119&rft.issn=0146-0404&rft.eissn=1552-5783&rft.coden=IOVSDA&rft_id=info:doi/10.1167/iovs.07-0353&rft_dat=%3Cproquest_cross%3E70170421%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70170421&rft_id=info:pmid/18172082&rfr_iscdi=true |