semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process
The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better per...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and bioengineering 2008-02, Vol.99 (3), p.728-733 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 733 |
|---|---|
| container_issue | 3 |
| container_start_page | 728 |
| container_title | Biotechnology and bioengineering |
| container_volume | 99 |
| creator | Aumann, Lars Morbidelli, Massimo |
| description | The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. Biotechnol. Bioeng. 2008;99: 728-733. © 2007 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.21585 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70169878</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20867207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5415-7c0bfda0674661654803cd65cc5f869619e17b3646c9eae46971b1689524d9433</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhSNERZfCgT8AERKoPaQd2_HYPsIKtpVaQOpWPVqO41Qu2XixE6D_noQsVEJCPc2M9M28eXpZ9oLAMQGgJ5Xvjynhkj_KFgSUKIAqeJwtAAALxhXdz56mdDuOQiI-yfaJQAkoySJbJ7fxNnS974YwpJwVNrTDpsttGLreRTvE6Lo-T6H9PtWbaGo_Ndsh-sZb0_vQ5YcXy8vVl6N8G4N1KT3L9hrTJvd8Vw-yq48f1svT4vzz6mz57rywvCS8EBaqpjaAokQkyEsJzNbIreWNRIVEOSIqhiVa5YwrUQlSEZSK07JWJWMH2dv57qj7bXCp1xufrGtb07nRjBZAUEkhHwQZ4UIKwAdBChIFBTGCr_8Bb8MQu9GtpoQJrqSc_juaIRtDStE1ehv9xsQ7TUBPyekxOf07uZF9uTs4VBtX35O7qEbgzQ4wyZq2iaazPt1zSqkS6OT1ZOZ--Nbd_V9Rvz9b_5Eu5g2fevfz74aJXzWK0Yu-_rTSp-XFNVO40pOtVzPfmKDNTRy_uLqkQBiALAlIyn4BC7TFZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213759883</pqid></control><display><type>article</type><title>semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Aumann, Lars ; Morbidelli, Massimo</creator><creatorcontrib>Aumann, Lars ; Morbidelli, Massimo</creatorcontrib><description>The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. Biotechnol. Bioeng. 2008;99: 728-733. © 2007 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.21585</identifier><identifier>PMID: 17680681</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>biochromatography ; Biological and medical sciences ; Biotechnology ; Calcitonin ; Chromatography ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Computer Simulation ; Computer-Aided Design ; countercurrent solvent gradient ; Equipment Design ; Equipment Failure Analysis ; Experiments ; Fundamental and applied biological sciences. Psychology ; MCSGP ; Methods. Procedures. Technologies ; Models, Chemical ; Others ; peptide purification ; Peptides ; semicontinuous ; SMB ; Solvents ; Solvents - chemistry ; three-fraction separation ; Various methods and equipments</subject><ispartof>Biotechnology and bioengineering, 2008-02, Vol.99 (3), p.728-733</ispartof><rights>Copyright © 2007 Wiley Periodicals, Inc.</rights><rights>2008 INIST-CNRS</rights><rights>(c) 2007 Wiley Periodicals, Inc.</rights><rights>Copyright John Wiley and Sons, Limited Feb 15, 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5415-7c0bfda0674661654803cd65cc5f869619e17b3646c9eae46971b1689524d9433</citedby><cites>FETCH-LOGICAL-c5415-7c0bfda0674661654803cd65cc5f869619e17b3646c9eae46971b1689524d9433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.21585$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.21585$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19994028$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17680681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aumann, Lars</creatorcontrib><creatorcontrib>Morbidelli, Massimo</creatorcontrib><title>semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. Biotechnol. Bioeng. 2008;99: 728-733. © 2007 Wiley Periodicals, Inc.</description><subject>biochromatography</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Calcitonin</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Computer Simulation</subject><subject>Computer-Aided Design</subject><subject>countercurrent solvent gradient</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Experiments</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>MCSGP</subject><subject>Methods. Procedures. Technologies</subject><subject>Models, Chemical</subject><subject>Others</subject><subject>peptide purification</subject><subject>Peptides</subject><subject>semicontinuous</subject><subject>SMB</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>three-fraction separation</subject><subject>Various methods and equipments</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhSNERZfCgT8AERKoPaQd2_HYPsIKtpVaQOpWPVqO41Qu2XixE6D_noQsVEJCPc2M9M28eXpZ9oLAMQGgJ5Xvjynhkj_KFgSUKIAqeJwtAAALxhXdz56mdDuOQiI-yfaJQAkoySJbJ7fxNnS974YwpJwVNrTDpsttGLreRTvE6Lo-T6H9PtWbaGo_Ndsh-sZb0_vQ5YcXy8vVl6N8G4N1KT3L9hrTJvd8Vw-yq48f1svT4vzz6mz57rywvCS8EBaqpjaAokQkyEsJzNbIreWNRIVEOSIqhiVa5YwrUQlSEZSK07JWJWMH2dv57qj7bXCp1xufrGtb07nRjBZAUEkhHwQZ4UIKwAdBChIFBTGCr_8Bb8MQu9GtpoQJrqSc_juaIRtDStE1ehv9xsQ7TUBPyekxOf07uZF9uTs4VBtX35O7qEbgzQ4wyZq2iaazPt1zSqkS6OT1ZOZ--Nbd_V9Rvz9b_5Eu5g2fevfz74aJXzWK0Yu-_rTSp-XFNVO40pOtVzPfmKDNTRy_uLqkQBiALAlIyn4BC7TFZA</recordid><startdate>20080215</startdate><enddate>20080215</enddate><creator>Aumann, Lars</creator><creator>Morbidelli, Massimo</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080215</creationdate><title>semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process</title><author>Aumann, Lars ; Morbidelli, Massimo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5415-7c0bfda0674661654803cd65cc5f869619e17b3646c9eae46971b1689524d9433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>biochromatography</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Calcitonin</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Computer Simulation</topic><topic>Computer-Aided Design</topic><topic>countercurrent solvent gradient</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Experiments</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>MCSGP</topic><topic>Methods. Procedures. Technologies</topic><topic>Models, Chemical</topic><topic>Others</topic><topic>peptide purification</topic><topic>Peptides</topic><topic>semicontinuous</topic><topic>SMB</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><topic>three-fraction separation</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aumann, Lars</creatorcontrib><creatorcontrib>Morbidelli, Massimo</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aumann, Lars</au><au>Morbidelli, Massimo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2008-02-15</date><risdate>2008</risdate><volume>99</volume><issue>3</issue><spage>728</spage><epage>733</epage><pages>728-733</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. Biotechnol. Bioeng. 2008;99: 728-733. © 2007 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17680681</pmid><doi>10.1002/bit.21585</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3592 |
| ispartof | Biotechnology and bioengineering, 2008-02, Vol.99 (3), p.728-733 |
| issn | 0006-3592 1097-0290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70169878 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | biochromatography Biological and medical sciences Biotechnology Calcitonin Chromatography Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Computer Simulation Computer-Aided Design countercurrent solvent gradient Equipment Design Equipment Failure Analysis Experiments Fundamental and applied biological sciences. Psychology MCSGP Methods. Procedures. Technologies Models, Chemical Others peptide purification Peptides semicontinuous SMB Solvents Solvents - chemistry three-fraction separation Various methods and equipments |
| title | semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T00%3A35%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=semicontinuous%203-column%20countercurrent%20solvent%20gradient%20purification%20(MCSGP)%20process&rft.jtitle=Biotechnology%20and%20bioengineering&rft.au=Aumann,%20Lars&rft.date=2008-02-15&rft.volume=99&rft.issue=3&rft.spage=728&rft.epage=733&rft.pages=728-733&rft.issn=0006-3592&rft.eissn=1097-0290&rft.coden=BIBIAU&rft_id=info:doi/10.1002/bit.21585&rft_dat=%3Cproquest_cross%3E20867207%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213759883&rft_id=info:pmid/17680681&rfr_iscdi=true |