Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer
Purpose: The diagnostic efficacy of Nuclear Matrix Protein-22 (NMP-22), bladder tumor antigen (BTA TRAK), and telomerase activity was evaluated in urine in a trial to assess their value in the detection of bladder cancer and to compare it to that of routine urine cytology. Subjects and Methods: The...
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| Veröffentlicht in: | Journal of Egyptian National Cancer Institute 2005-09, Vol.17 (3), p.193-202 |
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| creator | Abd al-Jawad, Manal A. Musa, Hibah S. Nasr, Maha I. al-Gemae, Imad H. Masud, Amr M. Ibrahim, Ibrahim Khalil al-Hifnawi, Nifin M. |
| description | Purpose: The diagnostic efficacy of Nuclear Matrix
Protein-22 (NMP-22), bladder tumor antigen (BTA TRAK),
and telomerase activity was evaluated in urine in a trial
to assess their value in the detection of bladder cancer
and to compare it to that of routine urine cytology.
Subjects and Methods: The study included 46 newly
diagnosed bladder cancer patients, diagnosed by cystoscopy
and histopathological typing, in addition to 20 patients
with benign bladder lesions and 20 healthy age and sex
matched volunteers as a control group. Fifty percent of
the cancer patients (23/46) had proven bilharzial history.
Most patients (27/46) had transitional cell carcinoma
(TCC), 17/46 had squamous cell carcinoma (SCC), while
only 2 patients had adenocarcinoma. A single freshly
voided urine sample (? 100ml) was collected from each
patient and control subject and aliquoted for each test. All
assays were conducted according to the manufacturer’s
guidelines and the results were compared to those of urine
cytology.
Results: The optimal cutoffs for NMP-22, BTA and
telomerase activity as calculated by ROC curves were
12.1 U/ml, 78 U/ml, 0.48 (Ratio) respectively. The levels
of the three parameters were significantly higher in the
malignant group compared to either the benign group or
normal controls, (p |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_70168929</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70168929</sourcerecordid><originalsourceid>FETCH-LOGICAL-e145t-7797d068dfce643d7d4b1adfbb96d7432aadc849bb835b59c26e3eb6a178afbd3</originalsourceid><addsrcrecordid>eNo90EtLxDAUBeAgijOO_gORrFxNIWnapFmOgy8YH-C4LjfJLVb6MkmF-fdWZnR1LoePuzhHZM5loRKttTyebs5ZwoRMZ-QshE_GpGQqPyUzLtUkcjUnb-u-HcBDrL-Rhji6He0r-vz0mqTpkkZs-hY9BFxS6By92a5o3dH4gdRhRBvrvvv1pgHn0FMLnUV_Tk4qaAJeHHJB3u9ut-uHZPNy_7hebRLkWR4TpbRyTBausigz4ZTLDAdXGaOlU5lIAZwtMm1MIXKTa5tKFGgkcFVAZZxYkOv938H3XyOGWLZ1sNg00GE_hlKxaQyd6gleHeBoWnTl4OsW_K78m2ECl3uAU48V_ItUF4KLH3YjY0g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70168929</pqid></control><display><type>article</type><title>Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Abd al-Jawad, Manal A. ; Musa, Hibah S. ; Nasr, Maha I. ; al-Gemae, Imad H. ; Masud, Amr M. ; Ibrahim, Ibrahim Khalil ; al-Hifnawi, Nifin M.</creator><creatorcontrib>Abd al-Jawad, Manal A. ; Musa, Hibah S. ; Nasr, Maha I. ; al-Gemae, Imad H. ; Masud, Amr M. ; Ibrahim, Ibrahim Khalil ; al-Hifnawi, Nifin M.</creatorcontrib><description>Purpose: The diagnostic efficacy of Nuclear Matrix
Protein-22 (NMP-22), bladder tumor antigen (BTA TRAK),
and telomerase activity was evaluated in urine in a trial
to assess their value in the detection of bladder cancer
and to compare it to that of routine urine cytology.
Subjects and Methods: The study included 46 newly
diagnosed bladder cancer patients, diagnosed by cystoscopy
and histopathological typing, in addition to 20 patients
with benign bladder lesions and 20 healthy age and sex
matched volunteers as a control group. Fifty percent of
the cancer patients (23/46) had proven bilharzial history.
Most patients (27/46) had transitional cell carcinoma
(TCC), 17/46 had squamous cell carcinoma (SCC), while
only 2 patients had adenocarcinoma. A single freshly
voided urine sample (? 100ml) was collected from each
patient and control subject and aliquoted for each test. All
assays were conducted according to the manufacturer’s
guidelines and the results were compared to those of urine
cytology.
Results: The optimal cutoffs for NMP-22, BTA and
telomerase activity as calculated by ROC curves were
12.1 U/ml, 78 U/ml, 0.48 (Ratio) respectively. The levels
of the three parameters were significantly higher in the
malignant group compared to either the benign group or
normal controls, (p<0.001) and the positive rates were
also higher in the malignant group for all 3 parameters.
The overall sensitivity of urine cytology, NMP-22, BTA
and telomerase was 54.3%, 91.3%, 100% and 80.4%
respectively. For bilharzial cancer bladder respective
sensitivities were 69.6%, 95.6%, 100% and 73.9%, while
for nonbilharzial cancer bladder the respective sensitivities
were 39.1%, 87%, 100% and 87%. The overall specificities
with urine cytology, NMP-22, BTA and telomerase was
100%, 87.5%, 92.5% and 95.0%, respectively. Combined
sensitivity of voided urine cytology with one or more of
the 3 biomarkers, or the use of these biomarkers in double
or triple combinations gave higher positivity than each
biomarker alone. Conclusion: BTA showed the highest sensitivity in
all the studied parameters in the bladder cancer group,
bilharzial bladder cancer subgroup, and non bilharzial
bladder subgroup, (100%), while the highest specificity
was recorded with urine cytology (100%), followed by
telomerase (95%), then BTA (92.5%), and lastly NMP-
22 (87.5%). Use of markers in combination with cytology,
or in a panel, improved the sensitivity, and specificity.</description><identifier>ISSN: 1110-0362</identifier><identifier>EISSN: 1687-9996</identifier><identifier>PMID: 16799657</identifier><language>eng</language><publisher>Cairo, Egypt: Cairo University, National Cancer Institute</publisher><subject>Adenocarcinoma - diagnosis ; Adenocarcinoma - urine ; Adult ; Aged ; Antigens, Neoplasm - urine ; Biomarkers, Tumor - urine ; Bladder ; Cancer ; Carcinoma, Squamous Cell - diagnosis ; Carcinoma, Squamous Cell - urine ; Carcinoma, Transitional Cell - diagnosis ; Carcinoma, Transitional Cell - urine ; Diagnosis ; Female ; Humans ; Male ; Middle Aged ; Nuclear Proteins - urine ; Schistosomiasis haematobia - complications ; Telomerase ; Telomerase - urine ; Tumor markers ; Urinary Bladder Neoplasms - diagnosis ; Urinary Bladder Neoplasms - parasitology ; Urinary Bladder Neoplasms - urine ; الأورام ; التشخيص ; السرطان ; المثانة</subject><ispartof>Journal of Egyptian National Cancer Institute, 2005-09, Vol.17 (3), p.193-202</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16799657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abd al-Jawad, Manal A.</creatorcontrib><creatorcontrib>Musa, Hibah S.</creatorcontrib><creatorcontrib>Nasr, Maha I.</creatorcontrib><creatorcontrib>al-Gemae, Imad H.</creatorcontrib><creatorcontrib>Masud, Amr M.</creatorcontrib><creatorcontrib>Ibrahim, Ibrahim Khalil</creatorcontrib><creatorcontrib>al-Hifnawi, Nifin M.</creatorcontrib><title>Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer</title><title>Journal of Egyptian National Cancer Institute</title><addtitle>J Egypt Natl Canc Inst</addtitle><description>Purpose: The diagnostic efficacy of Nuclear Matrix
Protein-22 (NMP-22), bladder tumor antigen (BTA TRAK),
and telomerase activity was evaluated in urine in a trial
to assess their value in the detection of bladder cancer
and to compare it to that of routine urine cytology.
Subjects and Methods: The study included 46 newly
diagnosed bladder cancer patients, diagnosed by cystoscopy
and histopathological typing, in addition to 20 patients
with benign bladder lesions and 20 healthy age and sex
matched volunteers as a control group. Fifty percent of
the cancer patients (23/46) had proven bilharzial history.
Most patients (27/46) had transitional cell carcinoma
(TCC), 17/46 had squamous cell carcinoma (SCC), while
only 2 patients had adenocarcinoma. A single freshly
voided urine sample (? 100ml) was collected from each
patient and control subject and aliquoted for each test. All
assays were conducted according to the manufacturer’s
guidelines and the results were compared to those of urine
cytology.
Results: The optimal cutoffs for NMP-22, BTA and
telomerase activity as calculated by ROC curves were
12.1 U/ml, 78 U/ml, 0.48 (Ratio) respectively. The levels
of the three parameters were significantly higher in the
malignant group compared to either the benign group or
normal controls, (p<0.001) and the positive rates were
also higher in the malignant group for all 3 parameters.
The overall sensitivity of urine cytology, NMP-22, BTA
and telomerase was 54.3%, 91.3%, 100% and 80.4%
respectively. For bilharzial cancer bladder respective
sensitivities were 69.6%, 95.6%, 100% and 73.9%, while
for nonbilharzial cancer bladder the respective sensitivities
were 39.1%, 87%, 100% and 87%. The overall specificities
with urine cytology, NMP-22, BTA and telomerase was
100%, 87.5%, 92.5% and 95.0%, respectively. Combined
sensitivity of voided urine cytology with one or more of
the 3 biomarkers, or the use of these biomarkers in double
or triple combinations gave higher positivity than each
biomarker alone. Conclusion: BTA showed the highest sensitivity in
all the studied parameters in the bladder cancer group,
bilharzial bladder cancer subgroup, and non bilharzial
bladder subgroup, (100%), while the highest specificity
was recorded with urine cytology (100%), followed by
telomerase (95%), then BTA (92.5%), and lastly NMP-
22 (87.5%). Use of markers in combination with cytology,
or in a panel, improved the sensitivity, and specificity.</description><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - urine</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Neoplasm - urine</subject><subject>Biomarkers, Tumor - urine</subject><subject>Bladder</subject><subject>Cancer</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Carcinoma, Squamous Cell - urine</subject><subject>Carcinoma, Transitional Cell - diagnosis</subject><subject>Carcinoma, Transitional Cell - urine</subject><subject>Diagnosis</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nuclear Proteins - urine</subject><subject>Schistosomiasis haematobia - complications</subject><subject>Telomerase</subject><subject>Telomerase - urine</subject><subject>Tumor markers</subject><subject>Urinary Bladder Neoplasms - diagnosis</subject><subject>Urinary Bladder Neoplasms - parasitology</subject><subject>Urinary Bladder Neoplasms - urine</subject><subject>الأورام</subject><subject>التشخيص</subject><subject>السرطان</subject><subject>المثانة</subject><issn>1110-0362</issn><issn>1687-9996</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90EtLxDAUBeAgijOO_gORrFxNIWnapFmOgy8YH-C4LjfJLVb6MkmF-fdWZnR1LoePuzhHZM5loRKttTyebs5ZwoRMZ-QshE_GpGQqPyUzLtUkcjUnb-u-HcBDrL-Rhji6He0r-vz0mqTpkkZs-hY9BFxS6By92a5o3dH4gdRhRBvrvvv1pgHn0FMLnUV_Tk4qaAJeHHJB3u9ut-uHZPNy_7hebRLkWR4TpbRyTBausigz4ZTLDAdXGaOlU5lIAZwtMm1MIXKTa5tKFGgkcFVAZZxYkOv938H3XyOGWLZ1sNg00GE_hlKxaQyd6gleHeBoWnTl4OsW_K78m2ECl3uAU48V_ItUF4KLH3YjY0g</recordid><startdate>200509</startdate><enddate>200509</enddate><creator>Abd al-Jawad, Manal A.</creator><creator>Musa, Hibah S.</creator><creator>Nasr, Maha I.</creator><creator>al-Gemae, Imad H.</creator><creator>Masud, Amr M.</creator><creator>Ibrahim, Ibrahim Khalil</creator><creator>al-Hifnawi, Nifin M.</creator><general>Cairo University, National Cancer Institute</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200509</creationdate><title>Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer</title><author>Abd al-Jawad, Manal A. ; Musa, Hibah S. ; Nasr, Maha I. ; al-Gemae, Imad H. ; Masud, Amr M. ; Ibrahim, Ibrahim Khalil ; al-Hifnawi, Nifin M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e145t-7797d068dfce643d7d4b1adfbb96d7432aadc849bb835b59c26e3eb6a178afbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - urine</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens, Neoplasm - urine</topic><topic>Biomarkers, Tumor - urine</topic><topic>Bladder</topic><topic>Cancer</topic><topic>Carcinoma, Squamous Cell - diagnosis</topic><topic>Carcinoma, Squamous Cell - urine</topic><topic>Carcinoma, Transitional Cell - diagnosis</topic><topic>Carcinoma, Transitional Cell - urine</topic><topic>Diagnosis</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nuclear Proteins - urine</topic><topic>Schistosomiasis haematobia - complications</topic><topic>Telomerase</topic><topic>Telomerase - urine</topic><topic>Tumor markers</topic><topic>Urinary Bladder Neoplasms - diagnosis</topic><topic>Urinary Bladder Neoplasms - parasitology</topic><topic>Urinary Bladder Neoplasms - urine</topic><topic>الأورام</topic><topic>التشخيص</topic><topic>السرطان</topic><topic>المثانة</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abd al-Jawad, Manal A.</creatorcontrib><creatorcontrib>Musa, Hibah S.</creatorcontrib><creatorcontrib>Nasr, Maha I.</creatorcontrib><creatorcontrib>al-Gemae, Imad H.</creatorcontrib><creatorcontrib>Masud, Amr M.</creatorcontrib><creatorcontrib>Ibrahim, Ibrahim Khalil</creatorcontrib><creatorcontrib>al-Hifnawi, Nifin M.</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Egyptian National Cancer Institute</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abd al-Jawad, Manal A.</au><au>Musa, Hibah S.</au><au>Nasr, Maha I.</au><au>al-Gemae, Imad H.</au><au>Masud, Amr M.</au><au>Ibrahim, Ibrahim Khalil</au><au>al-Hifnawi, Nifin M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer</atitle><jtitle>Journal of Egyptian National Cancer Institute</jtitle><addtitle>J Egypt Natl Canc Inst</addtitle><date>2005-09</date><risdate>2005</risdate><volume>17</volume><issue>3</issue><spage>193</spage><epage>202</epage><pages>193-202</pages><issn>1110-0362</issn><eissn>1687-9996</eissn><abstract>Purpose: The diagnostic efficacy of Nuclear Matrix
Protein-22 (NMP-22), bladder tumor antigen (BTA TRAK),
and telomerase activity was evaluated in urine in a trial
to assess their value in the detection of bladder cancer
and to compare it to that of routine urine cytology.
Subjects and Methods: The study included 46 newly
diagnosed bladder cancer patients, diagnosed by cystoscopy
and histopathological typing, in addition to 20 patients
with benign bladder lesions and 20 healthy age and sex
matched volunteers as a control group. Fifty percent of
the cancer patients (23/46) had proven bilharzial history.
Most patients (27/46) had transitional cell carcinoma
(TCC), 17/46 had squamous cell carcinoma (SCC), while
only 2 patients had adenocarcinoma. A single freshly
voided urine sample (? 100ml) was collected from each
patient and control subject and aliquoted for each test. All
assays were conducted according to the manufacturer’s
guidelines and the results were compared to those of urine
cytology.
Results: The optimal cutoffs for NMP-22, BTA and
telomerase activity as calculated by ROC curves were
12.1 U/ml, 78 U/ml, 0.48 (Ratio) respectively. The levels
of the three parameters were significantly higher in the
malignant group compared to either the benign group or
normal controls, (p<0.001) and the positive rates were
also higher in the malignant group for all 3 parameters.
The overall sensitivity of urine cytology, NMP-22, BTA
and telomerase was 54.3%, 91.3%, 100% and 80.4%
respectively. For bilharzial cancer bladder respective
sensitivities were 69.6%, 95.6%, 100% and 73.9%, while
for nonbilharzial cancer bladder the respective sensitivities
were 39.1%, 87%, 100% and 87%. The overall specificities
with urine cytology, NMP-22, BTA and telomerase was
100%, 87.5%, 92.5% and 95.0%, respectively. Combined
sensitivity of voided urine cytology with one or more of
the 3 biomarkers, or the use of these biomarkers in double
or triple combinations gave higher positivity than each
biomarker alone. Conclusion: BTA showed the highest sensitivity in
all the studied parameters in the bladder cancer group,
bilharzial bladder cancer subgroup, and non bilharzial
bladder subgroup, (100%), while the highest specificity
was recorded with urine cytology (100%), followed by
telomerase (95%), then BTA (92.5%), and lastly NMP-
22 (87.5%). Use of markers in combination with cytology,
or in a panel, improved the sensitivity, and specificity.</abstract><cop>Cairo, Egypt</cop><pub>Cairo University, National Cancer Institute</pub><pmid>16799657</pmid><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1110-0362 |
| ispartof | Journal of Egyptian National Cancer Institute, 2005-09, Vol.17 (3), p.193-202 |
| issn | 1110-0362 1687-9996 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70168929 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenocarcinoma - diagnosis Adenocarcinoma - urine Adult Aged Antigens, Neoplasm - urine Biomarkers, Tumor - urine Bladder Cancer Carcinoma, Squamous Cell - diagnosis Carcinoma, Squamous Cell - urine Carcinoma, Transitional Cell - diagnosis Carcinoma, Transitional Cell - urine Diagnosis Female Humans Male Middle Aged Nuclear Proteins - urine Schistosomiasis haematobia - complications Telomerase Telomerase - urine Tumor markers Urinary Bladder Neoplasms - diagnosis Urinary Bladder Neoplasms - parasitology Urinary Bladder Neoplasms - urine الأورام التشخيص السرطان المثانة |
| title | Comparative study of NMP-22, telomerase, and BTA in the detection of bladder cancer |
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