Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods
Mycobacterium avium subsp. avium (MAA) of serotype 2 and genotype IS901+ and IS1245+ was cultured from 21 naturally infected hens (Gallus domesticus) from one smallholder aviary. From a total of 330 samples taken from hens, 124 mycobacteria were detected. Out of which MAA was detected in 103 (35.7%)...
Gespeichert in:
Veröffentlicht in: | Veterinary microbiology 2008-02, Vol.127 (1-2), p.155-164 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 164 |
---|---|
container_issue | 1-2 |
container_start_page | 155 |
container_title | Veterinary microbiology |
container_volume | 127 |
creator | Shitaye, J.E Matlova, L Horvathova, A Moravkova, M Dvorska-Bartosova, L Treml, F Lamka, J Pavlik, I |
description | Mycobacterium avium subsp. avium (MAA) of serotype 2 and genotype IS901+ and IS1245+ was cultured from 21 naturally infected hens (Gallus domesticus) from one smallholder aviary. From a total of 330 samples taken from hens, 124 mycobacteria were detected. Out of which MAA was detected in 103 (35.7%) of 288 tissues, in 4 (19.0%) of 21 swabs of cloacae and in 9 (42.9%) of 21 faeces samples, 8 other conditionally pathogenic mycobacterial species were also isolated. Tuberculous (TB) lesions were found in the liver, spleen and intestinal organs of seven hens. The isolates of MAA (n = 58) from 16 infected hens (7 with TB lesions and 9 without TB lesions) were found to be of 3 IS901 RFLP types AE (n = 48), AD (n = 4) and E (n = 6), where these MAA isolates are highly virulent to hens. Mixed infections with IS901 RFLP types (AE and AD) and (AE and E) were also evident in seven hens. From a total of 35 examined environmental samples, 23 mycobacterial isolates were detected. Out of which four (17.4%) MAA isolates of IS901 RFLP type AE and 19 (82.6%) other isolates of conditionally pathogenic mycobacteria were detected. The finding of identical IS901 RFLP types from both tissues and faecal isolates confirms that infected domestic hens are the principal source of infection for other susceptible hosts and lead to the contamination of the surrounding environment. The presence of different IS901 RFLP types in tissue isolates may indicate the repeated incidence of MAA infection and the occurrence of polyclonal infection. |
doi_str_mv | 10.1016/j.vetmic.2007.07.026 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70167001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70167001</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-67c0e312811d8997a17fe93ae94adbe863080ef05eb103992c0e0d9119680fa63</originalsourceid><addsrcrecordid>eNpF0d2K1DAUB_AgijuuvoFobvTKqSfpR5JLWVxdGFFc97qk6elOxjadTdKBvosPa7pTWDgkHPidQ8ifkLcMMgas-nzIThgHazIOILKlePWMbJgU-ZaXBX9ONpALuWUsLy_IqxAOAFCoCl6SCyakAF5WG_Lvx2zGRpuI3k4D1aflDFMTjtnatDZEb5sp2tHREKfWYkuto5o6HSev-35ObYdpRUv36GjXj-Yv1e5RxT1SdCfrRzegi7SZqZn6NIefaEA_xvlo3f2jvrlVwOjv690vOmDcj214TV50ug_4Zr0vyd311z9X37e7n99urr7stqaAMm4rYQBzxiVjrVRKaCY6VLlGVei2QVnlIAE7KLFhkCvFE4dWMaYqCZ2u8kvy8bz36MeHCUOsBxsM9r12OE6hFum_BQBLsDhD48cQPHb10dtB-7lmUC-p1If6nEq9pFIvxZf979b9UzNg-zS0xpDAhxXoYHTfee2MDU9OKVlwJZN7f3adHmt975O5u-XpYQCykJLx_D-laqKX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70167001</pqid></control><display><type>article</type><title>Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Shitaye, J.E ; Matlova, L ; Horvathova, A ; Moravkova, M ; Dvorska-Bartosova, L ; Treml, F ; Lamka, J ; Pavlik, I</creator><creatorcontrib>Shitaye, J.E ; Matlova, L ; Horvathova, A ; Moravkova, M ; Dvorska-Bartosova, L ; Treml, F ; Lamka, J ; Pavlik, I</creatorcontrib><description>Mycobacterium avium subsp. avium (MAA) of serotype 2 and genotype IS901+ and IS1245+ was cultured from 21 naturally infected hens (Gallus domesticus) from one smallholder aviary. From a total of 330 samples taken from hens, 124 mycobacteria were detected. Out of which MAA was detected in 103 (35.7%) of 288 tissues, in 4 (19.0%) of 21 swabs of cloacae and in 9 (42.9%) of 21 faeces samples, 8 other conditionally pathogenic mycobacterial species were also isolated. Tuberculous (TB) lesions were found in the liver, spleen and intestinal organs of seven hens. The isolates of MAA (n = 58) from 16 infected hens (7 with TB lesions and 9 without TB lesions) were found to be of 3 IS901 RFLP types AE (n = 48), AD (n = 4) and E (n = 6), where these MAA isolates are highly virulent to hens. Mixed infections with IS901 RFLP types (AE and AD) and (AE and E) were also evident in seven hens. From a total of 35 examined environmental samples, 23 mycobacterial isolates were detected. Out of which four (17.4%) MAA isolates of IS901 RFLP type AE and 19 (82.6%) other isolates of conditionally pathogenic mycobacteria were detected. The finding of identical IS901 RFLP types from both tissues and faecal isolates confirms that infected domestic hens are the principal source of infection for other susceptible hosts and lead to the contamination of the surrounding environment. The presence of different IS901 RFLP types in tissue isolates may indicate the repeated incidence of MAA infection and the occurrence of polyclonal infection.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2007.07.026</identifier><identifier>PMID: 17870256</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Science</publisher><subject>Animals ; avian tuberculosis ; Bacterial diseases ; Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; Chickens ; Cloaca - microbiology ; Diagnosis ; disease prevalence ; disease reservoirs ; disease surveillance ; disease transmission ; environmental factors ; Environmental Microbiology ; Feces - microbiology ; Female ; Fundamental and applied biological sciences. Psychology ; genotype ; hens ; Human bacterial diseases ; Infectious diseases ; lesions (animal) ; Medical sciences ; Microbiology ; Miscellaneous ; molecular epidemiology ; Mycobacterium avium - classification ; Mycobacterium avium - isolation & purification ; Mycobacterium avium subsp. avium ; pathogen identification ; Polymorphism, Restriction Fragment Length ; poultry diseases ; Poultry Diseases - microbiology ; restriction fragment length polymorphism ; risk assessment ; serotypes ; Serotyping ; small-scale farming ; Tuberculosis and atypical mycobacterial infections ; Tuberculosis, Avian - microbiology ; Tuberculosis, Avian - pathology ; virulence ; zoonoses</subject><ispartof>Veterinary microbiology, 2008-02, Vol.127 (1-2), p.155-164</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-67c0e312811d8997a17fe93ae94adbe863080ef05eb103992c0e0d9119680fa63</citedby><cites>FETCH-LOGICAL-c405t-67c0e312811d8997a17fe93ae94adbe863080ef05eb103992c0e0d9119680fa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19984298$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17870256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shitaye, J.E</creatorcontrib><creatorcontrib>Matlova, L</creatorcontrib><creatorcontrib>Horvathova, A</creatorcontrib><creatorcontrib>Moravkova, M</creatorcontrib><creatorcontrib>Dvorska-Bartosova, L</creatorcontrib><creatorcontrib>Treml, F</creatorcontrib><creatorcontrib>Lamka, J</creatorcontrib><creatorcontrib>Pavlik, I</creatorcontrib><title>Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Mycobacterium avium subsp. avium (MAA) of serotype 2 and genotype IS901+ and IS1245+ was cultured from 21 naturally infected hens (Gallus domesticus) from one smallholder aviary. From a total of 330 samples taken from hens, 124 mycobacteria were detected. Out of which MAA was detected in 103 (35.7%) of 288 tissues, in 4 (19.0%) of 21 swabs of cloacae and in 9 (42.9%) of 21 faeces samples, 8 other conditionally pathogenic mycobacterial species were also isolated. Tuberculous (TB) lesions were found in the liver, spleen and intestinal organs of seven hens. The isolates of MAA (n = 58) from 16 infected hens (7 with TB lesions and 9 without TB lesions) were found to be of 3 IS901 RFLP types AE (n = 48), AD (n = 4) and E (n = 6), where these MAA isolates are highly virulent to hens. Mixed infections with IS901 RFLP types (AE and AD) and (AE and E) were also evident in seven hens. From a total of 35 examined environmental samples, 23 mycobacterial isolates were detected. Out of which four (17.4%) MAA isolates of IS901 RFLP type AE and 19 (82.6%) other isolates of conditionally pathogenic mycobacteria were detected. The finding of identical IS901 RFLP types from both tissues and faecal isolates confirms that infected domestic hens are the principal source of infection for other susceptible hosts and lead to the contamination of the surrounding environment. The presence of different IS901 RFLP types in tissue isolates may indicate the repeated incidence of MAA infection and the occurrence of polyclonal infection.</description><subject>Animals</subject><subject>avian tuberculosis</subject><subject>Bacterial diseases</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>Cloaca - microbiology</subject><subject>Diagnosis</subject><subject>disease prevalence</subject><subject>disease reservoirs</subject><subject>disease surveillance</subject><subject>disease transmission</subject><subject>environmental factors</subject><subject>Environmental Microbiology</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genotype</subject><subject>hens</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>lesions (animal)</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>molecular epidemiology</subject><subject>Mycobacterium avium - classification</subject><subject>Mycobacterium avium - isolation & purification</subject><subject>Mycobacterium avium subsp. avium</subject><subject>pathogen identification</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>poultry diseases</subject><subject>Poultry Diseases - microbiology</subject><subject>restriction fragment length polymorphism</subject><subject>risk assessment</subject><subject>serotypes</subject><subject>Serotyping</subject><subject>small-scale farming</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis, Avian - microbiology</subject><subject>Tuberculosis, Avian - pathology</subject><subject>virulence</subject><subject>zoonoses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0d2K1DAUB_AgijuuvoFobvTKqSfpR5JLWVxdGFFc97qk6elOxjadTdKBvosPa7pTWDgkHPidQ8ifkLcMMgas-nzIThgHazIOILKlePWMbJgU-ZaXBX9ONpALuWUsLy_IqxAOAFCoCl6SCyakAF5WG_Lvx2zGRpuI3k4D1aflDFMTjtnatDZEb5sp2tHREKfWYkuto5o6HSev-35ObYdpRUv36GjXj-Yv1e5RxT1SdCfrRzegi7SZqZn6NIefaEA_xvlo3f2jvrlVwOjv690vOmDcj214TV50ug_4Zr0vyd311z9X37e7n99urr7stqaAMm4rYQBzxiVjrVRKaCY6VLlGVei2QVnlIAE7KLFhkCvFE4dWMaYqCZ2u8kvy8bz36MeHCUOsBxsM9r12OE6hFum_BQBLsDhD48cQPHb10dtB-7lmUC-p1If6nEq9pFIvxZf979b9UzNg-zS0xpDAhxXoYHTfee2MDU9OKVlwJZN7f3adHmt975O5u-XpYQCykJLx_D-laqKX</recordid><startdate>20080205</startdate><enddate>20080205</enddate><creator>Shitaye, J.E</creator><creator>Matlova, L</creator><creator>Horvathova, A</creator><creator>Moravkova, M</creator><creator>Dvorska-Bartosova, L</creator><creator>Treml, F</creator><creator>Lamka, J</creator><creator>Pavlik, I</creator><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080205</creationdate><title>Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods</title><author>Shitaye, J.E ; Matlova, L ; Horvathova, A ; Moravkova, M ; Dvorska-Bartosova, L ; Treml, F ; Lamka, J ; Pavlik, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-67c0e312811d8997a17fe93ae94adbe863080ef05eb103992c0e0d9119680fa63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>avian tuberculosis</topic><topic>Bacterial diseases</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chickens</topic><topic>Cloaca - microbiology</topic><topic>Diagnosis</topic><topic>disease prevalence</topic><topic>disease reservoirs</topic><topic>disease surveillance</topic><topic>disease transmission</topic><topic>environmental factors</topic><topic>Environmental Microbiology</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genotype</topic><topic>hens</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>lesions (animal)</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>molecular epidemiology</topic><topic>Mycobacterium avium - classification</topic><topic>Mycobacterium avium - isolation & purification</topic><topic>Mycobacterium avium subsp. avium</topic><topic>pathogen identification</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>poultry diseases</topic><topic>Poultry Diseases - microbiology</topic><topic>restriction fragment length polymorphism</topic><topic>risk assessment</topic><topic>serotypes</topic><topic>Serotyping</topic><topic>small-scale farming</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tuberculosis, Avian - microbiology</topic><topic>Tuberculosis, Avian - pathology</topic><topic>virulence</topic><topic>zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shitaye, J.E</creatorcontrib><creatorcontrib>Matlova, L</creatorcontrib><creatorcontrib>Horvathova, A</creatorcontrib><creatorcontrib>Moravkova, M</creatorcontrib><creatorcontrib>Dvorska-Bartosova, L</creatorcontrib><creatorcontrib>Treml, F</creatorcontrib><creatorcontrib>Lamka, J</creatorcontrib><creatorcontrib>Pavlik, I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shitaye, J.E</au><au>Matlova, L</au><au>Horvathova, A</au><au>Moravkova, M</au><au>Dvorska-Bartosova, L</au><au>Treml, F</au><au>Lamka, J</au><au>Pavlik, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2008-02-05</date><risdate>2008</risdate><volume>127</volume><issue>1-2</issue><spage>155</spage><epage>164</epage><pages>155-164</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Mycobacterium avium subsp. avium (MAA) of serotype 2 and genotype IS901+ and IS1245+ was cultured from 21 naturally infected hens (Gallus domesticus) from one smallholder aviary. From a total of 330 samples taken from hens, 124 mycobacteria were detected. Out of which MAA was detected in 103 (35.7%) of 288 tissues, in 4 (19.0%) of 21 swabs of cloacae and in 9 (42.9%) of 21 faeces samples, 8 other conditionally pathogenic mycobacterial species were also isolated. Tuberculous (TB) lesions were found in the liver, spleen and intestinal organs of seven hens. The isolates of MAA (n = 58) from 16 infected hens (7 with TB lesions and 9 without TB lesions) were found to be of 3 IS901 RFLP types AE (n = 48), AD (n = 4) and E (n = 6), where these MAA isolates are highly virulent to hens. Mixed infections with IS901 RFLP types (AE and AD) and (AE and E) were also evident in seven hens. From a total of 35 examined environmental samples, 23 mycobacterial isolates were detected. Out of which four (17.4%) MAA isolates of IS901 RFLP type AE and 19 (82.6%) other isolates of conditionally pathogenic mycobacteria were detected. The finding of identical IS901 RFLP types from both tissues and faecal isolates confirms that infected domestic hens are the principal source of infection for other susceptible hosts and lead to the contamination of the surrounding environment. The presence of different IS901 RFLP types in tissue isolates may indicate the repeated incidence of MAA infection and the occurrence of polyclonal infection.</abstract><cop>Amsterdam</cop><pub>Elsevier Science</pub><pmid>17870256</pmid><doi>10.1016/j.vetmic.2007.07.026</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0378-1135 |
ispartof | Veterinary microbiology, 2008-02, Vol.127 (1-2), p.155-164 |
issn | 0378-1135 1873-2542 |
language | eng |
recordid | cdi_proquest_miscellaneous_70167001 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Animals avian tuberculosis Bacterial diseases Bacterial Typing Techniques Bacteriology Biological and medical sciences Chickens Cloaca - microbiology Diagnosis disease prevalence disease reservoirs disease surveillance disease transmission environmental factors Environmental Microbiology Feces - microbiology Female Fundamental and applied biological sciences. Psychology genotype hens Human bacterial diseases Infectious diseases lesions (animal) Medical sciences Microbiology Miscellaneous molecular epidemiology Mycobacterium avium - classification Mycobacterium avium - isolation & purification Mycobacterium avium subsp. avium pathogen identification Polymorphism, Restriction Fragment Length poultry diseases Poultry Diseases - microbiology restriction fragment length polymorphism risk assessment serotypes Serotyping small-scale farming Tuberculosis and atypical mycobacterial infections Tuberculosis, Avian - microbiology Tuberculosis, Avian - pathology virulence zoonoses |
title | Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T17%3A41%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mycobacterium%20avium%20subsp.%20avium%20distribution%20studied%20in%20a%20naturally%20infected%20hen%20flock%20and%20in%20the%20environment%20by%20culture,%20serotyping%20and%20IS901%20RFLP%20methods&rft.jtitle=Veterinary%20microbiology&rft.au=Shitaye,%20J.E&rft.date=2008-02-05&rft.volume=127&rft.issue=1-2&rft.spage=155&rft.epage=164&rft.pages=155-164&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/j.vetmic.2007.07.026&rft_dat=%3Cproquest_cross%3E70167001%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70167001&rft_id=info:pmid/17870256&rfr_iscdi=true |