Comparative proteomic and transcriptomic profiling of the human hepatocellular carcinoma
Proteome analysis of human hepatocellular carcinoma (HCC) was done using two-dimensional difference gel electrophoresis. To gain an understanding of the molecular events accompanying HCC development, we compared the protein expression profiles of HCC and non-HCC tissue from 14 patients to the mRNA e...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-02, Vol.366 (1), p.186-192 |
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| creator | Minagawa, Hirotaka Honda, Masao Miyazaki, Kenji Tabuse, Yo Teramoto, Reiji Yamashita, Taro Nishino, Ryuhei Takatori, Hajime Ueda, Teruyuki Kamijo, Ken’ichi Kaneko, Shuichi |
| description | Proteome analysis of human hepatocellular carcinoma (HCC) was done using two-dimensional difference gel electrophoresis. To gain an understanding of the molecular events accompanying HCC development, we compared the protein expression profiles of HCC and non-HCC tissue from 14 patients to the mRNA expression profiles of the same samples made from a cDNA microarray. A total of 125 proteins were identified, and the expression profiles of 93 proteins (149 spots) were compared to the mRNA expression profiles. The overall protein expression ratios correlated well with the mRNA ratios between HCC and non-HCC (Pearson’s correlation coefficient: r=0.73). Particularly, the HCC/non-HCC expression ratios of proteins involved in metabolic processes showed significant correlation to those of mRNA (r=0.9). A considerable number of proteins were expressed as multiple spots. Among them, several proteins showed spot-to-spot differences in expression level and their expression ratios between HCC and non-HCC poorly correlated to mRNA ratios. Such multi-spotted proteins might arise as a consequence of post-translational modifications. |
| doi_str_mv | 10.1016/j.bbrc.2007.11.101 |
| format | Article |
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To gain an understanding of the molecular events accompanying HCC development, we compared the protein expression profiles of HCC and non-HCC tissue from 14 patients to the mRNA expression profiles of the same samples made from a cDNA microarray. A total of 125 proteins were identified, and the expression profiles of 93 proteins (149 spots) were compared to the mRNA expression profiles. The overall protein expression ratios correlated well with the mRNA ratios between HCC and non-HCC (Pearson’s correlation coefficient: r=0.73). Particularly, the HCC/non-HCC expression ratios of proteins involved in metabolic processes showed significant correlation to those of mRNA (r=0.9). A considerable number of proteins were expressed as multiple spots. Among them, several proteins showed spot-to-spot differences in expression level and their expression ratios between HCC and non-HCC poorly correlated to mRNA ratios. Such multi-spotted proteins might arise as a consequence of post-translational modifications.</description><subject>Aged</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>cDNA microarray</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>Liver Neoplasms - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Proteome</subject><subject>Proteome - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptome</subject><subject>Tumor Cells, Cultured</subject><subject>Two-dimensional difference gel electrophoresis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFq3DAQhkVpabZJX6CH4FNv3s7IlmxBLmFJm0KglwZyE1p51NViW44kB_r2tbsLubWngX---Rk-xj4hbBFQfjlu9_totxyg2SKu2Ru2QVBQcoT6LdsAgCy5wqcL9iGlIwBiLdV7doEtSGiF2rCnXRgmE032L1RMMWQKg7eFGbsiRzMmG_2U_0bL0vnej7-K4Ip8oOIwD2YsDjSZHCz1_dybWFgTrR_DYK7YO2f6RB_P85I9fr37ubsvH358-767fSht1YpcSl53ilfCIe-azjrF26p2VSOE2UtHogNUjVASFVHryFaNpJYMcatULdFWl-zzqXf573mmlPXg0_qOGSnMSTeLKSlq8V-QQyt50_AF5CfQxpBSJKen6AcTf2sEvYrXR72K16t4jbhmy9H1uX3eD9S9npxNL8DNCaBFxounqJP1NFrqfCSbdRf8v_r_ABDVlX4</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Minagawa, Hirotaka</creator><creator>Honda, Masao</creator><creator>Miyazaki, Kenji</creator><creator>Tabuse, Yo</creator><creator>Teramoto, Reiji</creator><creator>Yamashita, Taro</creator><creator>Nishino, Ryuhei</creator><creator>Takatori, Hajime</creator><creator>Ueda, Teruyuki</creator><creator>Kamijo, Ken’ichi</creator><creator>Kaneko, Shuichi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20080201</creationdate><title>Comparative proteomic and transcriptomic profiling of the human hepatocellular carcinoma</title><author>Minagawa, Hirotaka ; Honda, Masao ; Miyazaki, Kenji ; Tabuse, Yo ; Teramoto, Reiji ; Yamashita, Taro ; Nishino, Ryuhei ; Takatori, Hajime ; Ueda, Teruyuki ; Kamijo, Ken’ichi ; Kaneko, Shuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-624d9235f12d7dcf92834f3755ab6fe5d019759619ee8fec376e8eae2c99461c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aged</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>cDNA microarray</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>Liver Neoplasms - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Proteome</topic><topic>Proteome - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Transcriptome</topic><topic>Tumor Cells, Cultured</topic><topic>Two-dimensional difference gel electrophoresis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minagawa, Hirotaka</creatorcontrib><creatorcontrib>Honda, Masao</creatorcontrib><creatorcontrib>Miyazaki, Kenji</creatorcontrib><creatorcontrib>Tabuse, Yo</creatorcontrib><creatorcontrib>Teramoto, Reiji</creatorcontrib><creatorcontrib>Yamashita, Taro</creatorcontrib><creatorcontrib>Nishino, Ryuhei</creatorcontrib><creatorcontrib>Takatori, Hajime</creatorcontrib><creatorcontrib>Ueda, Teruyuki</creatorcontrib><creatorcontrib>Kamijo, Ken’ichi</creatorcontrib><creatorcontrib>Kaneko, Shuichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minagawa, Hirotaka</au><au>Honda, Masao</au><au>Miyazaki, Kenji</au><au>Tabuse, Yo</au><au>Teramoto, Reiji</au><au>Yamashita, Taro</au><au>Nishino, Ryuhei</au><au>Takatori, Hajime</au><au>Ueda, Teruyuki</au><au>Kamijo, Ken’ichi</au><au>Kaneko, Shuichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomic and transcriptomic profiling of the human hepatocellular carcinoma</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>366</volume><issue>1</issue><spage>186</spage><epage>192</epage><pages>186-192</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Proteome analysis of human hepatocellular carcinoma (HCC) was done using two-dimensional difference gel electrophoresis. 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| subjects | Aged Biomarkers, Tumor - metabolism Carcinoma, Hepatocellular - metabolism cDNA microarray Female Gene Expression Profiling Hepatocellular carcinoma Humans Liver - metabolism Liver Neoplasms - metabolism Male Middle Aged Neoplasm Proteins - metabolism Proteome Proteome - metabolism Transcription Factors - metabolism Transcriptome Tumor Cells, Cultured Two-dimensional difference gel electrophoresis |
| title | Comparative proteomic and transcriptomic profiling of the human hepatocellular carcinoma |
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