Recombinant nucleocapsid-based ELISA for detection of IgG antibody to Rift Valley fever virus in African buffalo

Wild ruminants are thought to serve as natural hosts for Rift Valley fever virus (RVFV) but the role of these animals as reservoirs for RVFV during inter-epidemic periods and as amplifiers during epidemics is not well understood. An indirect enzyme-linked immunoassay (I-ELISA) based on the recombina...

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Veröffentlicht in:	Veterinary microbiology 2008-02, Vol.127 (1), p.21-28
Hauptverfasser:	Paweska, Janusz T., van Vuren, Petrus Jansen, Kemp, Alan, Buss, Peter, Bengis, Roy G., Gakuya, Francis, Breiman, Robert F., Njenga, M. Kariuki, Swanepoel, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	accuracy Africa African buffalo Animals Antibodies, Viral - blood antibody detection Biological and medical sciences Buffaloes - immunology Buffaloes - virology disease detection enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology IgG antibody immunoglobulin G Immunoglobulin G - blood Microbiology Miscellaneous Nucleocapsid - genetics Nucleocapsid - immunology nucleocapsid proteins Recombinant nucleocapsid ELISA recombinant proteins Recombinant Proteins - immunology reference standards Reproducibility of Results Rift Valley fever Rift Valley Fever - diagnosis Rift Valley Fever - veterinary Rift Valley Fever - virology Rift Valley fever virus Rift Valley fever virus - immunology Rift Valley fever virus - isolation & purification Ruminantia Sensitivity and Specificity serodiagnosis Syncerus caffer Validation validity Virology wild animals wildlife diseases
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