Dynamic in vivo metabolome response of Saccharomyces cerevisiae to a stepwise perturbation of the ATP requirement for benzoate export

Although much information is available on in vitro role of ATP in regulation, the in vivo kinetics of reactions in which ATP plays a role are only partly known. In order to study such reactions, it is therefore necessary to study the role of ATP in vivo. This study presents an in vivo, targeted pert...

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Veröffentlicht in:Biotechnology and bioengineering 2008-02, Vol.99 (2), p.421-441
Hauptverfasser: Kresnowati, M.T.A.P, van Winden, W.A, van Gulik, W.M, Heijnen, J.J
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container_start_page 421
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creator Kresnowati, M.T.A.P
van Winden, W.A
van Gulik, W.M
Heijnen, J.J
description Although much information is available on in vitro role of ATP in regulation, the in vivo kinetics of reactions in which ATP plays a role are only partly known. In order to study such reactions, it is therefore necessary to study the role of ATP in vivo. This study presents an in vivo, targeted perturbation of the ATP flux in aerobic glucose-limited chemostat cultures of Saccharomyces cerevisiae, which was accomplished by transiently (20 min) changing the extracellular undissociated benzoic acid concentration via the pH of the culture. The performed pH shifts resulted in, within about 20 s, a 40% decrease (pH upshift) or a 23% increase (pH downshift) of the calculated ATP consumption rate while the specific glucose uptake rate did not change because of the glucose-limited condition. The pH upshift resulted in a strong decrease in the glycolytic and TCA cycle fluxes; carbon and energy balances indicated an increased flux toward storage carbohydrates. As expected, the pH downshift leads to the opposite effects. Overall, consistent responses were observed in the metabolic fluxes, the off gas concentrations of O₂ and CO₂ and intracellular metabolite concentrations, except for the concentrations of adenosine nucleotides which unexpectedly only showed minor dynamics. This demonstrates that our knowledge of the regulation of the ATP level, the storage metabolism, and central carbon metabolism of yeast is still incomplete. The new dynamic metabolite datasets obtained in this study will prove of great value in developing kinetic models. Biotechnol. Bioeng. 2008;99: 421-441. © 2007 Wiley Periodicals, Inc.
doi_str_mv 10.1002/bit.21557
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subjects Adenosine triphosphatase
Adenosine Triphosphate - metabolism
Aerobiosis
ATP
Benzoates - metabolism
benzoic acid
Biological and medical sciences
Biotechnology
Carbon - metabolism
Cell culture
Fermentation
Fundamental and applied biological sciences. Psychology
Glucose
Kinetics
Metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Studies
Systems Biology
targetted perturbation
Yeast
title Dynamic in vivo metabolome response of Saccharomyces cerevisiae to a stepwise perturbation of the ATP requirement for benzoate export
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