A PCR–restriction fragment length polymorphism assay to genotype human metapneumovirus

Human metapneumovirus (hMPV) genotypes A and B show epidemiological and probably clinical differences. This report describes a fast and simple PCR–restriction fragment length polymorphism (PCR-RFLP) assay, involving digestion of the fusion protein gene with Tsp509I, that allows lineages A1, A2, B1 a...

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Veröffentlicht in:	Clinical microbiology and infection 2008-01, Vol.14 (1), p.91-93
Hauptverfasser:	Montes, M., Vicente, D., Esnal, O., Cilla, G., Pérez-Trallero, E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplified Fragment Length Polymorphism Analysis - methods Biological and medical sciences Child Fundamental and applied biological sciences. Psychology Fusion protein gene Genetics Genotype genotypes Human metapneumovirus human metapneumovuris Humans identification lineages Metapneumovirus - classification Metapneumovirus - genetics Metapneumovirus - isolation & purification Microbiology Molecular Epidemiology - methods PCR-RFLP Viral Fusion Proteins - genetics Virology
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