Intranasal immunization with Naegleria fowleri lysates and Cry1Ac induces metaplasia in the olfactory epithelium and increases IgA secretion
According to previous reports, intranasal administration of the Cry1Ac protein alone or with amoebic lysates increases protection against Naegleria fowleri meningoencephalitis in mice, apparently by eliciting IgA responses in the nasal mucosa. In the current study, we performed an immunohistochemica...
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| Veröffentlicht in: | Parasite immunology 2008, Vol.30 (1), p.31-38 |
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| creator | JARILLO-LUNA, A MORENO-FIERROS, L CAMPOS-RODRÍGUEZ, R RODRÍGUEZ-MONROY, M.A LARA-PADILLA, E ROJAS-HERNÁNDEZ, S |
| description | According to previous reports, intranasal administration of the Cry1Ac protein alone or with amoebic lysates increases protection against Naegleria fowleri meningoencephalitis in mice, apparently by eliciting IgA responses in the nasal mucosa. In the current study, we performed an immunohistochemical analysis of IgA in the nasal mucosa of mice immunized intranasally with Cry1Ac, and amoebic lysates or a combination of both. The animals were sacrificed 24 h after the last immunization or after an intranasal lethal challenge with N. fowleri. Our results indicate that all of the intranasal immunizations provoked an increase in areas with metaplasia in the olfactory epithelium, allowing for secretion of IgA. As a result, IgA antibodies were found interacting with trophozoites in the nasal lumen, and there was a marked increase of IgA in the metaplasic epithelium. On the other hand in nonimmunized mice trophozoites were observed invading the nasal mucosa, which was not the case for immunized mice. Our results suggest that intranasal immunization provokes cellular changes in the olfactory epithelium, leading to greater protection against N. fowleri that is probably caused by an increased secretion of IgA. The increased IgA response induced in the nasal mucosa by immunization probably impedes both amoebic adhesion and subsequent invasion of the parasite to the nasal epithelium. |
| doi_str_mv | 10.1111/j.1365-3024.2007.00999.x |
| format | Article |
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In the current study, we performed an immunohistochemical analysis of IgA in the nasal mucosa of mice immunized intranasally with Cry1Ac, and amoebic lysates or a combination of both. The animals were sacrificed 24 h after the last immunization or after an intranasal lethal challenge with N. fowleri. Our results indicate that all of the intranasal immunizations provoked an increase in areas with metaplasia in the olfactory epithelium, allowing for secretion of IgA. As a result, IgA antibodies were found interacting with trophozoites in the nasal lumen, and there was a marked increase of IgA in the metaplasic epithelium. On the other hand in nonimmunized mice trophozoites were observed invading the nasal mucosa, which was not the case for immunized mice. Our results suggest that intranasal immunization provokes cellular changes in the olfactory epithelium, leading to greater protection against N. fowleri that is probably caused by an increased secretion of IgA. The increased IgA response induced in the nasal mucosa by immunization probably impedes both amoebic adhesion and subsequent invasion of the parasite to the nasal epithelium.</description><identifier>ISSN: 0141-9838</identifier><identifier>EISSN: 1365-3024</identifier><identifier>DOI: 10.1111/j.1365-3024.2007.00999.x</identifier><identifier>PMID: 18086014</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adjuvants, Immunologic ; Administration, Intranasal ; Amebiasis - immunology ; Amebiasis - parasitology ; Animals ; Antibodies, Protozoan - analysis ; Antigens, Protozoan - immunology ; Bacterial Proteins - immunology ; Bacterial Toxins - immunology ; Endotoxins - immunology ; Hemolysin Proteins - immunology ; IgA ; Immunization ; Immunoglobulin A, Secretory - analysis ; Male ; Meningoencephalitis - immunology ; Meningoencephalitis - parasitology ; Metaplasia ; Mice ; Mice, Inbred BALB C ; Naegleria fowleri ; Naegleria fowleri - immunology ; Nasal mucosa ; Nasal Mucosa - immunology ; Nasal Mucosa - parasitology ; Olfactory Mucosa - immunology ; Recombinant Proteins - immunology</subject><ispartof>Parasite immunology, 2008, Vol.30 (1), p.31-38</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4219-1ead0174bb5514be1a06beadf7b56f204dbbf1e27ecf5b68b1cf6d3de7499f153</citedby><cites>FETCH-LOGICAL-c4219-1ead0174bb5514be1a06beadf7b56f204dbbf1e27ecf5b68b1cf6d3de7499f153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3024.2007.00999.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3024.2007.00999.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,4024,27923,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18086014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JARILLO-LUNA, A</creatorcontrib><creatorcontrib>MORENO-FIERROS, L</creatorcontrib><creatorcontrib>CAMPOS-RODRÍGUEZ, R</creatorcontrib><creatorcontrib>RODRÍGUEZ-MONROY, M.A</creatorcontrib><creatorcontrib>LARA-PADILLA, E</creatorcontrib><creatorcontrib>ROJAS-HERNÁNDEZ, S</creatorcontrib><title>Intranasal immunization with Naegleria fowleri lysates and Cry1Ac induces metaplasia in the olfactory epithelium and increases IgA secretion</title><title>Parasite immunology</title><addtitle>Parasite Immunol</addtitle><description>According to previous reports, intranasal administration of the Cry1Ac protein alone or with amoebic lysates increases protection against Naegleria fowleri meningoencephalitis in mice, apparently by eliciting IgA responses in the nasal mucosa. In the current study, we performed an immunohistochemical analysis of IgA in the nasal mucosa of mice immunized intranasally with Cry1Ac, and amoebic lysates or a combination of both. The animals were sacrificed 24 h after the last immunization or after an intranasal lethal challenge with N. fowleri. Our results indicate that all of the intranasal immunizations provoked an increase in areas with metaplasia in the olfactory epithelium, allowing for secretion of IgA. As a result, IgA antibodies were found interacting with trophozoites in the nasal lumen, and there was a marked increase of IgA in the metaplasic epithelium. On the other hand in nonimmunized mice trophozoites were observed invading the nasal mucosa, which was not the case for immunized mice. Our results suggest that intranasal immunization provokes cellular changes in the olfactory epithelium, leading to greater protection against N. fowleri that is probably caused by an increased secretion of IgA. The increased IgA response induced in the nasal mucosa by immunization probably impedes both amoebic adhesion and subsequent invasion of the parasite to the nasal epithelium.</description><subject>Adjuvants, Immunologic</subject><subject>Administration, Intranasal</subject><subject>Amebiasis - immunology</subject><subject>Amebiasis - parasitology</subject><subject>Animals</subject><subject>Antibodies, Protozoan - analysis</subject><subject>Antigens, Protozoan - immunology</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Toxins - immunology</subject><subject>Endotoxins - immunology</subject><subject>Hemolysin Proteins - immunology</subject><subject>IgA</subject><subject>Immunization</subject><subject>Immunoglobulin A, Secretory - analysis</subject><subject>Male</subject><subject>Meningoencephalitis - immunology</subject><subject>Meningoencephalitis - parasitology</subject><subject>Metaplasia</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Naegleria fowleri</subject><subject>Naegleria fowleri - immunology</subject><subject>Nasal mucosa</subject><subject>Nasal Mucosa - immunology</subject><subject>Nasal Mucosa - parasitology</subject><subject>Olfactory Mucosa - immunology</subject><subject>Recombinant Proteins - immunology</subject><issn>0141-9838</issn><issn>1365-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUktv1DAQthAVXQp_AXzilmDn4SQSl9WKx0qlrQQ9W-NkvPXKcRY70Tb8Bn40TncFR_DF45nvIc1nQihnKY_n_T7luSiTnGVFmjFWpYw1TZM-PiOrP4PnZMV4wZOmzutL8jKEPWM8z0T-glzymtUiTlfk19aNHhwEsNT0_eTMTxjN4OjRjA_0BnBn0RugejguBbVzgBEDBdfRjZ_5uqXGdVMbWz2OcLAQIto4Oj4gHayGdhz8TPEQ5dCaqX9iGtd6hBBJ292aBoyvxfQVudBgA74-31fk_tPH75svyfXt5-1mfZ20RcabhCN0jFeFUmXJC4UcmFCxpytVCp2xolNKc8wqbHWpRK14q0WXd1gVTaN5mV-Rdyfdgx9-TBhG2ZvQorXgcJiCrOJqRCX4P4HRSzBR1xFYn4CtH0LwqOXBmx78LDmTS2RyL5dk5JKMXCKTT5HJx0h9c_aYVI_dX-I5owj4cAIcjcX5v4Xl3fZrLCL97YmuYZCw8ybI-29Z_AqM1bkQcR2_ASPBsYo</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>JARILLO-LUNA, A</creator><creator>MORENO-FIERROS, L</creator><creator>CAMPOS-RODRÍGUEZ, R</creator><creator>RODRÍGUEZ-MONROY, M.A</creator><creator>LARA-PADILLA, E</creator><creator>ROJAS-HERNÁNDEZ, S</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Intranasal immunization with Naegleria fowleri lysates and Cry1Ac induces metaplasia in the olfactory epithelium and increases IgA secretion</title><author>JARILLO-LUNA, A ; MORENO-FIERROS, L ; CAMPOS-RODRÍGUEZ, R ; RODRÍGUEZ-MONROY, M.A ; LARA-PADILLA, E ; ROJAS-HERNÁNDEZ, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4219-1ead0174bb5514be1a06beadf7b56f204dbbf1e27ecf5b68b1cf6d3de7499f153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adjuvants, Immunologic</topic><topic>Administration, Intranasal</topic><topic>Amebiasis - immunology</topic><topic>Amebiasis - parasitology</topic><topic>Animals</topic><topic>Antibodies, Protozoan - analysis</topic><topic>Antigens, Protozoan - immunology</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Toxins - immunology</topic><topic>Endotoxins - immunology</topic><topic>Hemolysin Proteins - immunology</topic><topic>IgA</topic><topic>Immunization</topic><topic>Immunoglobulin A, Secretory - analysis</topic><topic>Male</topic><topic>Meningoencephalitis - immunology</topic><topic>Meningoencephalitis - parasitology</topic><topic>Metaplasia</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Naegleria fowleri</topic><topic>Naegleria fowleri - immunology</topic><topic>Nasal mucosa</topic><topic>Nasal Mucosa - immunology</topic><topic>Nasal Mucosa - parasitology</topic><topic>Olfactory Mucosa - immunology</topic><topic>Recombinant Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JARILLO-LUNA, A</creatorcontrib><creatorcontrib>MORENO-FIERROS, L</creatorcontrib><creatorcontrib>CAMPOS-RODRÍGUEZ, R</creatorcontrib><creatorcontrib>RODRÍGUEZ-MONROY, M.A</creatorcontrib><creatorcontrib>LARA-PADILLA, E</creatorcontrib><creatorcontrib>ROJAS-HERNÁNDEZ, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasite immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JARILLO-LUNA, A</au><au>MORENO-FIERROS, L</au><au>CAMPOS-RODRÍGUEZ, R</au><au>RODRÍGUEZ-MONROY, M.A</au><au>LARA-PADILLA, E</au><au>ROJAS-HERNÁNDEZ, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intranasal immunization with Naegleria fowleri lysates and Cry1Ac induces metaplasia in the olfactory epithelium and increases IgA secretion</atitle><jtitle>Parasite immunology</jtitle><addtitle>Parasite Immunol</addtitle><date>2008</date><risdate>2008</risdate><volume>30</volume><issue>1</issue><spage>31</spage><epage>38</epage><pages>31-38</pages><issn>0141-9838</issn><eissn>1365-3024</eissn><abstract>According to previous reports, intranasal administration of the Cry1Ac protein alone or with amoebic lysates increases protection against Naegleria fowleri meningoencephalitis in mice, apparently by eliciting IgA responses in the nasal mucosa. In the current study, we performed an immunohistochemical analysis of IgA in the nasal mucosa of mice immunized intranasally with Cry1Ac, and amoebic lysates or a combination of both. The animals were sacrificed 24 h after the last immunization or after an intranasal lethal challenge with N. fowleri. Our results indicate that all of the intranasal immunizations provoked an increase in areas with metaplasia in the olfactory epithelium, allowing for secretion of IgA. As a result, IgA antibodies were found interacting with trophozoites in the nasal lumen, and there was a marked increase of IgA in the metaplasic epithelium. On the other hand in nonimmunized mice trophozoites were observed invading the nasal mucosa, which was not the case for immunized mice. Our results suggest that intranasal immunization provokes cellular changes in the olfactory epithelium, leading to greater protection against N. fowleri that is probably caused by an increased secretion of IgA. The increased IgA response induced in the nasal mucosa by immunization probably impedes both amoebic adhesion and subsequent invasion of the parasite to the nasal epithelium.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18086014</pmid><doi>10.1111/j.1365-3024.2007.00999.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Parasite immunology, 2008, Vol.30 (1), p.31-38 |
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| subjects | Adjuvants, Immunologic Administration, Intranasal Amebiasis - immunology Amebiasis - parasitology Animals Antibodies, Protozoan - analysis Antigens, Protozoan - immunology Bacterial Proteins - immunology Bacterial Toxins - immunology Endotoxins - immunology Hemolysin Proteins - immunology IgA Immunization Immunoglobulin A, Secretory - analysis Male Meningoencephalitis - immunology Meningoencephalitis - parasitology Metaplasia Mice Mice, Inbred BALB C Naegleria fowleri Naegleria fowleri - immunology Nasal mucosa Nasal Mucosa - immunology Nasal Mucosa - parasitology Olfactory Mucosa - immunology Recombinant Proteins - immunology |
| title | Intranasal immunization with Naegleria fowleri lysates and Cry1Ac induces metaplasia in the olfactory epithelium and increases IgA secretion |
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