Monitoring of Brucella reactor does following milk examination using different techniques
Milk samples from 129 does were collected and monitored for Brucella antibodies using immunological tests such as Milk Ring Test (MRT), Whey Agglutination Test (WAT), Whey Antiglobulin Coombs Test (WCT) and milk ELISA (m ELISA) using Brucella Periplasmic protein antigen. Results obtained from these...
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| Veröffentlicht in: | Pakistan journal of biological sciences 2007-01, Vol.10 (2), p.240-244 |
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| creator | el-Razik, K A Abd Ghazi, Y A Salama, E M |
| description | Milk samples from 129 does were collected and monitored for Brucella antibodies using immunological tests such as Milk Ring Test (MRT), Whey Agglutination Test (WAT), Whey Antiglobulin Coombs Test (WCT) and milk ELISA (m ELISA) using Brucella Periplasmic protein antigen. Results obtained from these tests were compared to PCR and bacterial isolation. The highest incidence of positive reactors was given by Whey Antiglobulin and Whey Agglutination Test (9.3%) while the lowest incidence was given by bacterial isolation (Br. melitensis biovars 3, 3.8%). PCR showed the highest agreement with the bacterial isolation, while WAT and WCT showed the lowest one. PCR showed a high sensitivity of 1 x 10 B. melitensis CFU mL(-1) of milk. The results of mELISA here suggests its efficiency to be used as a screening test and/or confirmatory test, while the modified MRT still need more investigations to diagnosis caprine brucellosis. |
| doi_str_mv | 10.3923/pjbs.2007.240.244 |
| format | Article |
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Results obtained from these tests were compared to PCR and bacterial isolation. The highest incidence of positive reactors was given by Whey Antiglobulin and Whey Agglutination Test (9.3%) while the lowest incidence was given by bacterial isolation (Br. melitensis biovars 3, 3.8%). PCR showed the highest agreement with the bacterial isolation, while WAT and WCT showed the lowest one. PCR showed a high sensitivity of 1 x 10 B. melitensis CFU mL(-1) of milk. The results of mELISA here suggests its efficiency to be used as a screening test and/or confirmatory test, while the modified MRT still need more investigations to diagnosis caprine brucellosis.</description><identifier>ISSN: 1028-8880</identifier><identifier>DOI: 10.3923/pjbs.2007.240.244</identifier><identifier>PMID: 19070022</identifier><language>eng</language><publisher>Pakistan</publisher><subject>Animals ; Antibodies, Bacterial - blood ; Brucella ; Brucella - genetics ; Brucella - immunology ; Brucellosis - diagnosis ; Brucellosis - veterinary ; Egypt ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Goat Diseases - diagnosis ; Goat Diseases - microbiology ; Goats ; Milk - microbiology ; Polymerase Chain Reaction - veterinary</subject><ispartof>Pakistan journal of biological sciences, 2007-01, Vol.10 (2), p.240-244</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2884-eecc86427e8af06744178a1e5f78aac7c20a72ec98c89274eb7b4adfd3a6b5e03</citedby><cites>FETCH-LOGICAL-c2884-eecc86427e8af06744178a1e5f78aac7c20a72ec98c89274eb7b4adfd3a6b5e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4124,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19070022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>el-Razik, K A Abd</creatorcontrib><creatorcontrib>Ghazi, Y A</creatorcontrib><creatorcontrib>Salama, E M</creatorcontrib><title>Monitoring of Brucella reactor does following milk examination using different techniques</title><title>Pakistan journal of biological sciences</title><addtitle>Pak J Biol Sci</addtitle><description>Milk samples from 129 does were collected and monitored for Brucella antibodies using immunological tests such as Milk Ring Test (MRT), Whey Agglutination Test (WAT), Whey Antiglobulin Coombs Test (WCT) and milk ELISA (m ELISA) using Brucella Periplasmic protein antigen. Results obtained from these tests were compared to PCR and bacterial isolation. The highest incidence of positive reactors was given by Whey Antiglobulin and Whey Agglutination Test (9.3%) while the lowest incidence was given by bacterial isolation (Br. melitensis biovars 3, 3.8%). PCR showed the highest agreement with the bacterial isolation, while WAT and WCT showed the lowest one. PCR showed a high sensitivity of 1 x 10 B. melitensis CFU mL(-1) of milk. The results of mELISA here suggests its efficiency to be used as a screening test and/or confirmatory test, while the modified MRT still need more investigations to diagnosis caprine brucellosis.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Brucella</subject><subject>Brucella - genetics</subject><subject>Brucella - immunology</subject><subject>Brucellosis - diagnosis</subject><subject>Brucellosis - veterinary</subject><subject>Egypt</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>Goat Diseases - diagnosis</subject><subject>Goat Diseases - microbiology</subject><subject>Goats</subject><subject>Milk - microbiology</subject><subject>Polymerase Chain Reaction - veterinary</subject><issn>1028-8880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhj2AaCn8ABaUiS3l7DixMwLiSypigYEpcpwzuCRxsRMB_56YVmJkOL3S6bk73UPICYVlVrLsfLOuw5IBiCXjMBXfI3MKTKZSSpiRwxDWAFwwIQ_IjJYgABibk5cH19vBedu_Js4kl37U2LYq8aj01E4ahyExrm3dZ0Q6274n-KU626vBuj4ZQ2w31hj02A_JgPqttx8jhiOyb1Qb8HiXC_J8c_10dZeuHm_vry5WqWZS8hRRa1lwJlAqA4XgnAqpKOZmCqWFZqAEQ11KLUsmONai5qoxTaaKOkfIFuRsu3fjXbw7VJ0Nv0_06MZQCaBZWRb5vyCLRkQeQboFtXcheDTVxttO-e-KQhVlV1F25EU1yZ6KTzOnu-Vj3WHzN7Eznf0AFbt_Sw</recordid><startdate>20070115</startdate><enddate>20070115</enddate><creator>el-Razik, K A Abd</creator><creator>Ghazi, Y A</creator><creator>Salama, E M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070115</creationdate><title>Monitoring of Brucella reactor does following milk examination using different techniques</title><author>el-Razik, K A Abd ; Ghazi, Y A ; Salama, E M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2884-eecc86427e8af06744178a1e5f78aac7c20a72ec98c89274eb7b4adfd3a6b5e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Brucella</topic><topic>Brucella - genetics</topic><topic>Brucella - immunology</topic><topic>Brucellosis - diagnosis</topic><topic>Brucellosis - veterinary</topic><topic>Egypt</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>Goat Diseases - diagnosis</topic><topic>Goat Diseases - microbiology</topic><topic>Goats</topic><topic>Milk - microbiology</topic><topic>Polymerase Chain Reaction - veterinary</topic><toplevel>online_resources</toplevel><creatorcontrib>el-Razik, K A Abd</creatorcontrib><creatorcontrib>Ghazi, Y A</creatorcontrib><creatorcontrib>Salama, E M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>el-Razik, K A Abd</au><au>Ghazi, Y A</au><au>Salama, E M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring of Brucella reactor does following milk examination using different techniques</atitle><jtitle>Pakistan journal of biological sciences</jtitle><addtitle>Pak J Biol Sci</addtitle><date>2007-01-15</date><risdate>2007</risdate><volume>10</volume><issue>2</issue><spage>240</spage><epage>244</epage><pages>240-244</pages><issn>1028-8880</issn><abstract>Milk samples from 129 does were collected and monitored for Brucella antibodies using immunological tests such as Milk Ring Test (MRT), Whey Agglutination Test (WAT), Whey Antiglobulin Coombs Test (WCT) and milk ELISA (m ELISA) using Brucella Periplasmic protein antigen. Results obtained from these tests were compared to PCR and bacterial isolation. The highest incidence of positive reactors was given by Whey Antiglobulin and Whey Agglutination Test (9.3%) while the lowest incidence was given by bacterial isolation (Br. melitensis biovars 3, 3.8%). PCR showed the highest agreement with the bacterial isolation, while WAT and WCT showed the lowest one. PCR showed a high sensitivity of 1 x 10 B. melitensis CFU mL(-1) of milk. The results of mELISA here suggests its efficiency to be used as a screening test and/or confirmatory test, while the modified MRT still need more investigations to diagnosis caprine brucellosis.</abstract><cop>Pakistan</cop><pmid>19070022</pmid><doi>10.3923/pjbs.2007.240.244</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pakistan journal of biological sciences, 2007-01, Vol.10 (2), p.240-244 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Science Alert |
| subjects | Animals Antibodies, Bacterial - blood Brucella Brucella - genetics Brucella - immunology Brucellosis - diagnosis Brucellosis - veterinary Egypt Enzyme-Linked Immunosorbent Assay - veterinary Female Goat Diseases - diagnosis Goat Diseases - microbiology Goats Milk - microbiology Polymerase Chain Reaction - veterinary |
| title | Monitoring of Brucella reactor does following milk examination using different techniques |
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