Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes
We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella‐containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salm...
Gespeichert in:
| Veröffentlicht in: | Methods in Enzymology 2005, Vol.403, p.295-309 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 309 |
|---|---|
| container_issue | |
| container_start_page | 295 |
| container_title | Methods in Enzymology |
| container_volume | 403 |
| creator | Parashuraman, Seetharaman Mukhopadhyay, Amitabha |
| description | We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella‐containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salmonella‐containing phagosomes recruit more Rab5 than dead Salmonella‐containing phagosomes. Recruitment of Rab5 on live Salmonella‐containing phagosomes depends on the presence of viable bacteria in the phagosomes. Subsequently, we identified an effector molecule of Salmonella, SopE, which specifically binds Rab5. Moreover, SopE is found to be a specific nucleotide exchange factor for Rab5 and thereby retains Rab5 in an active conformation. Activated Rab5 on Salmonella‐containing phagosomes promotes fusion with early endosomes and thus avoids transport to the lysosomes. |
| doi_str_mv | 10.1016/S0076-6879(05)03025-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_70129842</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0076687905030259</els_id><sourcerecordid>70129842</sourcerecordid><originalsourceid>FETCH-LOGICAL-c317t-12469ded8fb76e80fafdd0d81ececf791234d2355d03e3efee8c664daad77a773</originalsourceid><addsrcrecordid>eNo9kU1OHDEQha2QCEZkjgDyCiWLTvzT_lshNIIkElIQk6wtj13NGHXbQ9uNxC5HyBlzkvQASW1qUd8rPb2H0Aklnyih8vOaECUbqZX5QMRHwgkTjXmDFlQI1Sij9QFaGqUJZVQzTTR5ixb_JUdoWco9mac1TDB-iI6obBUXRi7Q9qIU94RdCvhqSr7GnFyPb8a8g7FGKDh3eJ13lzgmXLeAb8GPU6wDpLo_3bqNwDnhteuHnKDv3Z9fv1c5VRdTTHf4ZuvucskDlPfoXef6AsvXfYx-Xl3-WH1trr9_-ba6uG48p6o2lLXSBAi62ygJmnSuC4EETcGD75ShjLeBcSEC4cChA9BeyjY4F5RySvFjdPbydzfmhwlKtUMsfu8sQZ6KVXNIRrdsBk9fwWkzQLC7MQ5ufLL_spmB8xcAZruPEUZbfITkIcQRfLUhR0uJ3Rdknwuy-7QtEfa5IGv4X9X4gTk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70129842</pqid></control><display><type>article</type><title>Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes</title><source>ScienceDirect eBooks</source><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Parashuraman, Seetharaman ; Mukhopadhyay, Amitabha</creator><creatorcontrib>Parashuraman, Seetharaman ; Mukhopadhyay, Amitabha</creatorcontrib><description>We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella‐containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salmonella‐containing phagosomes recruit more Rab5 than dead Salmonella‐containing phagosomes. Recruitment of Rab5 on live Salmonella‐containing phagosomes depends on the presence of viable bacteria in the phagosomes. Subsequently, we identified an effector molecule of Salmonella, SopE, which specifically binds Rab5. Moreover, SopE is found to be a specific nucleotide exchange factor for Rab5 and thereby retains Rab5 in an active conformation. Activated Rab5 on Salmonella‐containing phagosomes promotes fusion with early endosomes and thus avoids transport to the lysosomes.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 9780121828080</identifier><identifier>ISBN: 0121828085</identifier><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/S0076-6879(05)03025-9</identifier><identifier>PMID: 16473596</identifier><language>eng</language><publisher>United States: Elsevier Science & Technology</publisher><subject>Animals ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Cell Line ; Mice ; Phagosomes - microbiology ; Protein Binding ; rab5 GTP-Binding Proteins - metabolism ; rab5 GTP-Binding Proteins - physiology ; Salmonella - isolation & purification</subject><ispartof>Methods in Enzymology, 2005, Vol.403, p.295-309</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-12469ded8fb76e80fafdd0d81ececf791234d2355d03e3efee8c664daad77a773</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0076687905030259$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,775,776,780,789,3446,3537,4010,11269,27902,27903,27904,45789,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16473596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parashuraman, Seetharaman</creatorcontrib><creatorcontrib>Mukhopadhyay, Amitabha</creatorcontrib><title>Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes</title><title>Methods in Enzymology</title><addtitle>Methods Enzymol</addtitle><description>We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella‐containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salmonella‐containing phagosomes recruit more Rab5 than dead Salmonella‐containing phagosomes. Recruitment of Rab5 on live Salmonella‐containing phagosomes depends on the presence of viable bacteria in the phagosomes. Subsequently, we identified an effector molecule of Salmonella, SopE, which specifically binds Rab5. Moreover, SopE is found to be a specific nucleotide exchange factor for Rab5 and thereby retains Rab5 in an active conformation. Activated Rab5 on Salmonella‐containing phagosomes promotes fusion with early endosomes and thus avoids transport to the lysosomes.</description><subject>Animals</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Cell Line</subject><subject>Mice</subject><subject>Phagosomes - microbiology</subject><subject>Protein Binding</subject><subject>rab5 GTP-Binding Proteins - metabolism</subject><subject>rab5 GTP-Binding Proteins - physiology</subject><subject>Salmonella - isolation & purification</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>9780121828080</isbn><isbn>0121828085</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1OHDEQha2QCEZkjgDyCiWLTvzT_lshNIIkElIQk6wtj13NGHXbQ9uNxC5HyBlzkvQASW1qUd8rPb2H0Aklnyih8vOaECUbqZX5QMRHwgkTjXmDFlQI1Sij9QFaGqUJZVQzTTR5ixb_JUdoWco9mac1TDB-iI6obBUXRi7Q9qIU94RdCvhqSr7GnFyPb8a8g7FGKDh3eJ13lzgmXLeAb8GPU6wDpLo_3bqNwDnhteuHnKDv3Z9fv1c5VRdTTHf4ZuvucskDlPfoXef6AsvXfYx-Xl3-WH1trr9_-ba6uG48p6o2lLXSBAi62ygJmnSuC4EETcGD75ShjLeBcSEC4cChA9BeyjY4F5RySvFjdPbydzfmhwlKtUMsfu8sQZ6KVXNIRrdsBk9fwWkzQLC7MQ5ufLL_spmB8xcAZruPEUZbfITkIcQRfLUhR0uJ3Rdknwuy-7QtEfa5IGv4X9X4gTk</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Parashuraman, Seetharaman</creator><creator>Mukhopadhyay, Amitabha</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes</title><author>Parashuraman, Seetharaman ; Mukhopadhyay, Amitabha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-12469ded8fb76e80fafdd0d81ececf791234d2355d03e3efee8c664daad77a773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Cell Line</topic><topic>Mice</topic><topic>Phagosomes - microbiology</topic><topic>Protein Binding</topic><topic>rab5 GTP-Binding Proteins - metabolism</topic><topic>rab5 GTP-Binding Proteins - physiology</topic><topic>Salmonella - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parashuraman, Seetharaman</creatorcontrib><creatorcontrib>Mukhopadhyay, Amitabha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parashuraman, Seetharaman</au><au>Mukhopadhyay, Amitabha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>2005</date><risdate>2005</risdate><volume>403</volume><spage>295</spage><epage>309</epage><pages>295-309</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>9780121828080</isbn><isbn>0121828085</isbn><abstract>We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella‐containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salmonella‐containing phagosomes recruit more Rab5 than dead Salmonella‐containing phagosomes. Recruitment of Rab5 on live Salmonella‐containing phagosomes depends on the presence of viable bacteria in the phagosomes. Subsequently, we identified an effector molecule of Salmonella, SopE, which specifically binds Rab5. Moreover, SopE is found to be a specific nucleotide exchange factor for Rab5 and thereby retains Rab5 in an active conformation. Activated Rab5 on Salmonella‐containing phagosomes promotes fusion with early endosomes and thus avoids transport to the lysosomes.</abstract><cop>United States</cop><pub>Elsevier Science & Technology</pub><pmid>16473596</pmid><doi>10.1016/S0076-6879(05)03025-9</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 2005, Vol.403, p.295-309 |
| issn | 0076-6879 1557-7988 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70129842 |
| source | ScienceDirect eBooks; MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacterial Proteins - metabolism Bacterial Proteins - physiology Cell Line Mice Phagosomes - microbiology Protein Binding rab5 GTP-Binding Proteins - metabolism rab5 GTP-Binding Proteins - physiology Salmonella - isolation & purification |
| title | Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella‐Containing Phagosomes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T18%3A43%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assay%20and%20Functional%20Properties%20of%20SopE%20in%20the%20Recruitment%20of%20Rab5%20on%20Salmonella%E2%80%90Containing%20Phagosomes&rft.jtitle=Methods%20in%20Enzymology&rft.au=Parashuraman,%20Seetharaman&rft.date=2005&rft.volume=403&rft.spage=295&rft.epage=309&rft.pages=295-309&rft.issn=0076-6879&rft.eissn=1557-7988&rft.isbn=9780121828080&rft.isbn_list=0121828085&rft_id=info:doi/10.1016/S0076-6879(05)03025-9&rft_dat=%3Cproquest_pubme%3E70129842%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70129842&rft_id=info:pmid/16473596&rft_els_id=S0076687905030259&rfr_iscdi=true |