Rickettsia felis, from Culture to Genome Sequencing
: Rickettsia felis has been recently cultured in XTC2 cells. This allows production of enough bacteria to create a genomic bank and to sequence it. The chromosome of R. felis is longer than that of previously sequenced rickettsiae and it possess 2 plasmids. Microscopically, this bacterium exhibits t...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2005-12, Vol.1063 (1), p.26-34 |
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| container_title | Annals of the New York Academy of Sciences |
| container_volume | 1063 |
| creator | OGATA, H ROBERT, C AUDIC, S ROBINEAU, S BLANC, G FOURNIER, P E RENESTO, P CLAVERIE, J M RAOULT, D |
| description | : Rickettsia felis has been recently cultured in XTC2 cells. This allows production of enough bacteria to create a genomic bank and to sequence it. The chromosome of R. felis is longer than that of previously sequenced rickettsiae and it possess 2 plasmids. Microscopically, this bacterium exhibits two forms of pili: one resembles a conjugative pilus and another forms hair‐like projections that may play a role in pathogenicity. R. felis also exhibits several copies of ankyrin‐repeat genes and tetratricopeptide encoding gene that are specifically linked to pathogenic host‐associated bacteria. It also contains toxin‐antitoxin system encoding genes that are extremely rare in intracellular bacteria and may be linked to plasmid maintenance. |
| doi_str_mv | 10.1196/annals.1355.004 |
| format | Article |
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This allows production of enough bacteria to create a genomic bank and to sequence it. The chromosome of R. felis is longer than that of previously sequenced rickettsiae and it possess 2 plasmids. Microscopically, this bacterium exhibits two forms of pili: one resembles a conjugative pilus and another forms hair‐like projections that may play a role in pathogenicity. R. felis also exhibits several copies of ankyrin‐repeat genes and tetratricopeptide encoding gene that are specifically linked to pathogenic host‐associated bacteria. It also contains toxin‐antitoxin system encoding genes that are extremely rare in intracellular bacteria and may be linked to plasmid maintenance.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1196/annals.1355.004</identifier><identifier>PMID: 16481487</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Bacteriological Techniques ; DNA, Bacterial - analysis ; Felis ; gene ; genome sequencing ; Genome, Bacterial ; Humans ; pili ; plasmid ; Rickettsia felis ; Rickettsia felis - genetics ; Rickettsia felis - growth & development ; Rickettsia felis - pathogenicity ; Rickettsia felis - ultrastructure ; Sequence Analysis, DNA</subject><ispartof>Annals of the New York Academy of Sciences, 2005-12, Vol.1063 (1), p.26-34</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4086-ee136c7ecdd97cfb18c97bb8fb8ad79002de8c8149b7665522e4da12db91e28c3</citedby><cites>FETCH-LOGICAL-c4086-ee136c7ecdd97cfb18c97bb8fb8ad79002de8c8149b7665522e4da12db91e28c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1355.004$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1355.004$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16481487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OGATA, H</creatorcontrib><creatorcontrib>ROBERT, C</creatorcontrib><creatorcontrib>AUDIC, S</creatorcontrib><creatorcontrib>ROBINEAU, S</creatorcontrib><creatorcontrib>BLANC, G</creatorcontrib><creatorcontrib>FOURNIER, P E</creatorcontrib><creatorcontrib>RENESTO, P</creatorcontrib><creatorcontrib>CLAVERIE, J M</creatorcontrib><creatorcontrib>RAOULT, D</creatorcontrib><title>Rickettsia felis, from Culture to Genome Sequencing</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: Rickettsia felis has been recently cultured in XTC2 cells. This allows production of enough bacteria to create a genomic bank and to sequence it. The chromosome of R. felis is longer than that of previously sequenced rickettsiae and it possess 2 plasmids. Microscopically, this bacterium exhibits two forms of pili: one resembles a conjugative pilus and another forms hair‐like projections that may play a role in pathogenicity. R. felis also exhibits several copies of ankyrin‐repeat genes and tetratricopeptide encoding gene that are specifically linked to pathogenic host‐associated bacteria. It also contains toxin‐antitoxin system encoding genes that are extremely rare in intracellular bacteria and may be linked to plasmid maintenance.</description><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>DNA, Bacterial - analysis</subject><subject>Felis</subject><subject>gene</subject><subject>genome sequencing</subject><subject>Genome, Bacterial</subject><subject>Humans</subject><subject>pili</subject><subject>plasmid</subject><subject>Rickettsia felis</subject><subject>Rickettsia felis - genetics</subject><subject>Rickettsia felis - growth & development</subject><subject>Rickettsia felis - pathogenicity</subject><subject>Rickettsia felis - ultrastructure</subject><subject>Sequence Analysis, DNA</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwkAURidGI4iu3ZmuXFmYRzuPJaKigWAiGONq0k5vTaUP7LRR_r1DSnTJ6m7O9917D0KXBA8JUXwUlWWU2yFhYTjEODhCfSIC5XPO6DHqYyyELxVlPXRm7SfGhMpAnKIe4YEkgRR9xF4ys4amsVnkpZBn9sZL66rwJm3etDV4TeVNoawK8Jbw1UJpsvLjHJ2kbitc7OcAvT7cryaP_vx5-jQZz30TYMl9AMK4EWCSRAmTxkQaJeJYprGMEqEwpglI4-5QseA8DCmFIIkITWJFgErDBui6693UldttG11k1kCeRyVUrdXC_aNCrA6CFFOKlQodOOpAU1fW1pDqTZ0VUb3VBOudUN0J1Tuh2gl1iat9dRsXkPzze4MOYB3wneWwPdSnF-_jJeUu5XepzDbw85eK6rXmgolQvy2menXLZzyYUX3HfgHiQZEw</recordid><startdate>200512</startdate><enddate>200512</enddate><creator>OGATA, H</creator><creator>ROBERT, C</creator><creator>AUDIC, S</creator><creator>ROBINEAU, S</creator><creator>BLANC, G</creator><creator>FOURNIER, P E</creator><creator>RENESTO, P</creator><creator>CLAVERIE, J M</creator><creator>RAOULT, D</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200512</creationdate><title>Rickettsia felis, from Culture to Genome Sequencing</title><author>OGATA, H ; ROBERT, C ; AUDIC, S ; ROBINEAU, S ; BLANC, G ; FOURNIER, P E ; RENESTO, P ; CLAVERIE, J M ; RAOULT, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4086-ee136c7ecdd97cfb18c97bb8fb8ad79002de8c8149b7665522e4da12db91e28c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bacteriological Techniques</topic><topic>DNA, Bacterial - analysis</topic><topic>Felis</topic><topic>gene</topic><topic>genome sequencing</topic><topic>Genome, Bacterial</topic><topic>Humans</topic><topic>pili</topic><topic>plasmid</topic><topic>Rickettsia felis</topic><topic>Rickettsia felis - genetics</topic><topic>Rickettsia felis - growth & development</topic><topic>Rickettsia felis - pathogenicity</topic><topic>Rickettsia felis - ultrastructure</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OGATA, H</creatorcontrib><creatorcontrib>ROBERT, C</creatorcontrib><creatorcontrib>AUDIC, S</creatorcontrib><creatorcontrib>ROBINEAU, S</creatorcontrib><creatorcontrib>BLANC, G</creatorcontrib><creatorcontrib>FOURNIER, P E</creatorcontrib><creatorcontrib>RENESTO, P</creatorcontrib><creatorcontrib>CLAVERIE, J M</creatorcontrib><creatorcontrib>RAOULT, D</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OGATA, H</au><au>ROBERT, C</au><au>AUDIC, S</au><au>ROBINEAU, S</au><au>BLANC, G</au><au>FOURNIER, P E</au><au>RENESTO, P</au><au>CLAVERIE, J M</au><au>RAOULT, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rickettsia felis, from Culture to Genome Sequencing</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2005-12</date><risdate>2005</risdate><volume>1063</volume><issue>1</issue><spage>26</spage><epage>34</epage><pages>26-34</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: Rickettsia felis has been recently cultured in XTC2 cells. 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| ispartof | Annals of the New York Academy of Sciences, 2005-12, Vol.1063 (1), p.26-34 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Bacteriological Techniques DNA, Bacterial - analysis Felis gene genome sequencing Genome, Bacterial Humans pili plasmid Rickettsia felis Rickettsia felis - genetics Rickettsia felis - growth & development Rickettsia felis - pathogenicity Rickettsia felis - ultrastructure Sequence Analysis, DNA |
| title | Rickettsia felis, from Culture to Genome Sequencing |
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