Inhibition effect of antisense Bmi-1 on Jurkat cells
To investigate whether antisense Bmi-1 plasmid could inhibit the proliferation of Jurkat cells. The antisense plasmid was constructed by PCR amplification of a 171 bp segment spanning Bmi-1 start codon and zinc finger structure and the PCR product was subsequently inserted reversely to plasmid pLNCX...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2005-09, Vol.26 (9), p.554-556 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Liu, Wei-hong Meng, Xiu-xiang Liu, Dan-dan Shan, Lu-juan Zhao, Xin-yu |
| description | To investigate whether antisense Bmi-1 plasmid could inhibit the proliferation of Jurkat cells.
The antisense plasmid was constructed by PCR amplification of a 171 bp segment spanning Bmi-1 start codon and zinc finger structure and the PCR product was subsequently inserted reversely to plasmid pLNCX2. The final construct was confirmed through restriction enzyme digestion. G418 was added into the medium after the plasmid was successfully introduced into Jurkat cells by using lipofectin-mediated DNA transfection. The proliferation of Jurkat cells were determined by MTT and colony formation assays. Cell cycle was determined by flow cytometry. The p16 expression of Jurkat cells was studied by immunofluorescent histochemistry.
The growth rate of antisense Bmi-1 transfected Jurkat cells was significantly lower than that of the controls, and the colony forming capacity of the transfected cells decreased significantly (P < 0.01), the colony numbers being (90.7 +/- 9.07)/10(3) cells, (83.3 +/- 6.11)/10(3) cells and (5 |
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The antisense plasmid was constructed by PCR amplification of a 171 bp segment spanning Bmi-1 start codon and zinc finger structure and the PCR product was subsequently inserted reversely to plasmid pLNCX2. The final construct was confirmed through restriction enzyme digestion. G418 was added into the medium after the plasmid was successfully introduced into Jurkat cells by using lipofectin-mediated DNA transfection. The proliferation of Jurkat cells were determined by MTT and colony formation assays. Cell cycle was determined by flow cytometry. The p16 expression of Jurkat cells was studied by immunofluorescent histochemistry.
The growth rate of antisense Bmi-1 transfected Jurkat cells was significantly lower than that of the controls, and the colony forming capacity of the transfected cells decreased significantly (P < 0.01), the colony numbers being (90.7 +/- 9.07)/10(3) cells, (83.3 +/- 6.11)/10(3) cells and (5</description><identifier>ISSN: 0253-2727</identifier><identifier>PMID: 16468335</identifier><language>chi</language><publisher>China</publisher><subject>Cell Cycle ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Genetic Vectors ; Humans ; Jurkat Cells ; Nuclear Proteins - genetics ; Oligonucleotides, Antisense - genetics ; Plasmids - genetics ; Polycomb Repressive Complex 1 ; Proto-Oncogene Proteins - genetics ; Repressor Proteins - genetics ; Transfection</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2005-09, Vol.26 (9), p.554-556</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16468335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Wei-hong</creatorcontrib><creatorcontrib>Meng, Xiu-xiang</creatorcontrib><creatorcontrib>Liu, Dan-dan</creatorcontrib><creatorcontrib>Shan, Lu-juan</creatorcontrib><creatorcontrib>Zhao, Xin-yu</creatorcontrib><title>Inhibition effect of antisense Bmi-1 on Jurkat cells</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To investigate whether antisense Bmi-1 plasmid could inhibit the proliferation of Jurkat cells.
The antisense plasmid was constructed by PCR amplification of a 171 bp segment spanning Bmi-1 start codon and zinc finger structure and the PCR product was subsequently inserted reversely to plasmid pLNCX2. The final construct was confirmed through restriction enzyme digestion. G418 was added into the medium after the plasmid was successfully introduced into Jurkat cells by using lipofectin-mediated DNA transfection. The proliferation of Jurkat cells were determined by MTT and colony formation assays. Cell cycle was determined by flow cytometry. The p16 expression of Jurkat cells was studied by immunofluorescent histochemistry.
The growth rate of antisense Bmi-1 transfected Jurkat cells was significantly lower than that of the controls, and the colony forming capacity of the transfected cells decreased significantly (P < 0.01), the colony numbers being (90.7 +/- 9.07)/10(3) cells, (83.3 +/- 6.11)/10(3) cells and (5</description><subject>Cell Cycle</subject><subject>Cell Proliferation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Jurkat Cells</subject><subject>Nuclear Proteins - genetics</subject><subject>Oligonucleotides, Antisense - genetics</subject><subject>Plasmids - genetics</subject><subject>Polycomb Repressive Complex 1</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Transfection</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j01LAzEYhHNQbKn9C5KTt4Ukb5LNHrX40VLwouflzRcG98tN9uC_t8V6moF5GGauyJoJBZWoRb0i25yTZYqDNgDshqy4lmer1kTuh89kU0njQEOMwRU6RopDSTkMOdDHPlWcnsLDMn9hoS50Xb4l1xG7HLYX3ZCP56f33Wt1fHvZ7x6O1cSFLBWAlY41QjXeiOgQNEK0AhrjVW0xYmQepdOKaSVroyRnzmvudYMeHCJsyP1f7zSP30vIpe1TPi_AIYxLbmvGhTKNOYF3F3CxffDtNKce55_2_yf8Am9MTWM</recordid><startdate>200509</startdate><enddate>200509</enddate><creator>Liu, Wei-hong</creator><creator>Meng, Xiu-xiang</creator><creator>Liu, Dan-dan</creator><creator>Shan, Lu-juan</creator><creator>Zhao, Xin-yu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200509</creationdate><title>Inhibition effect of antisense Bmi-1 on Jurkat cells</title><author>Liu, Wei-hong ; Meng, Xiu-xiang ; Liu, Dan-dan ; Shan, Lu-juan ; Zhao, Xin-yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-33b4c09259d82fca36a3fb2398d57bafaf0da4c650654785410cd61d69ad3caa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2005</creationdate><topic>Cell Cycle</topic><topic>Cell Proliferation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Jurkat Cells</topic><topic>Nuclear Proteins - genetics</topic><topic>Oligonucleotides, Antisense - genetics</topic><topic>Plasmids - genetics</topic><topic>Polycomb Repressive Complex 1</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wei-hong</creatorcontrib><creatorcontrib>Meng, Xiu-xiang</creatorcontrib><creatorcontrib>Liu, Dan-dan</creatorcontrib><creatorcontrib>Shan, Lu-juan</creatorcontrib><creatorcontrib>Zhao, Xin-yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wei-hong</au><au>Meng, Xiu-xiang</au><au>Liu, Dan-dan</au><au>Shan, Lu-juan</au><au>Zhao, Xin-yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition effect of antisense Bmi-1 on Jurkat cells</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2005-09</date><risdate>2005</risdate><volume>26</volume><issue>9</issue><spage>554</spage><epage>556</epage><pages>554-556</pages><issn>0253-2727</issn><abstract>To investigate whether antisense Bmi-1 plasmid could inhibit the proliferation of Jurkat cells.
The antisense plasmid was constructed by PCR amplification of a 171 bp segment spanning Bmi-1 start codon and zinc finger structure and the PCR product was subsequently inserted reversely to plasmid pLNCX2. The final construct was confirmed through restriction enzyme digestion. G418 was added into the medium after the plasmid was successfully introduced into Jurkat cells by using lipofectin-mediated DNA transfection. The proliferation of Jurkat cells were determined by MTT and colony formation assays. Cell cycle was determined by flow cytometry. The p16 expression of Jurkat cells was studied by immunofluorescent histochemistry.
The growth rate of antisense Bmi-1 transfected Jurkat cells was significantly lower than that of the controls, and the colony forming capacity of the transfected cells decreased significantly (P < 0.01), the colony numbers being (90.7 +/- 9.07)/10(3) cells, (83.3 +/- 6.11)/10(3) cells and (5</abstract><cop>China</cop><pmid>16468335</pmid><tpages>3</tpages></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2005-09, Vol.26 (9), p.554-556 |
| issn | 0253-2727 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_70125898 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Cell Cycle Cell Proliferation Gene Expression Regulation, Neoplastic Genetic Vectors Humans Jurkat Cells Nuclear Proteins - genetics Oligonucleotides, Antisense - genetics Plasmids - genetics Polycomb Repressive Complex 1 Proto-Oncogene Proteins - genetics Repressor Proteins - genetics Transfection |
| title | Inhibition effect of antisense Bmi-1 on Jurkat cells |
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