Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis
Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 c...
Gespeichert in:
| Veröffentlicht in: | American journal of respiratory and critical care medicine 1998-12, Vol.158 (6), p.1945-1950 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1950 |
|---|---|
| container_issue | 6 |
| container_start_page | 1945 |
| container_title | American journal of respiratory and critical care medicine |
| container_volume | 158 |
| creator | VIGNOLA, A. M RICCOBONO, L MIRABELLA, A PROFITA, M CHANEZ, P BELLIA, V MAUTINO, G DACCARDI, P BOUSQUET, J BONSIGNORE, G |
| description | Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 control, 31 untreated asthmatic, and 16 chronic bronchitic subjects. We first performed zymographic analysis to identify the profile of MMPs. Zymography revealed a similar MMPs profile in all populations studied and that MMP-9 was the major enzyme released. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and of its inhibitor TIMP-1 and evaluated whether airflow limitation may be associated with an imbalance between these enzymes. MMP-9 and TIMP-1 concentrations were greater in sputum of patients with asthma and chronic bronchitis than in control subjects. The molar ratio between MMP-9 and TIMP-1 was lower in asthmatics and chronic bronchitics than in control subjects, and positively correlated with FEV1 values. In asthma, MMP-9 levels were significantly correlated with the number of macrophages and neutrophils. This study shows that airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1 which may have a role in the pathogenesis of ECM remodeling and airflow obstruction. |
| doi_str_mv | 10.1164/ajrccm.158.6.9803014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70120934</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70120934</sourcerecordid><originalsourceid>FETCH-LOGICAL-c331t-19eaa5f91dd85e6b13580094a9a642a6bf76a7ed66914e98903d07358a0302683</originalsourceid><addsrcrecordid>eNptkc1KxTAQhYMo_r-BQhbirtekSdNkKeIfCC5UcBemaUojbXNNUsSH8J2N3KIbVzMw35xhzkHohJIVpYJfwFswZlzRSq7ESknCCOVbaJ9WrCq4qsl27knNCs7V6x46iPGNEFpKSnbRrpK8LhXZR19P6znNIx5tgmHw6-CTdRNEW6iL5GKcLXZT7xqXfMC--4ejOEByHhsfgh0g2Yg_XOoxuNAN_gP7JqYwm4xMWQpDTP0IGKYWmz74yRnc5GJ6l88doZ0OhmiPl3qIXm6un6_uiofH2_ury4fCMEZTQZUFqDpF21ZWVjSUVZIQxUGB4CWIpqsF1LYVQlFulVSEtdmJSkI2qRSSHaLzjW7-4322MenRRWOHASbr56jrbBRRjGeQb0ATfIzBdnod3AjhU1Oif1LQmxR0TkELvaSQ104X_bkZbfu7tNie52fLHKKBoQswGRf_tEWZOcm-AVk9lOM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70120934</pqid></control><display><type>article</type><title>Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis</title><source>Journals@Ovid Ovid Autoload</source><source>MEDLINE</source><source>American Thoracic Society (ATS) Journals Online</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>VIGNOLA, A. M ; RICCOBONO, L ; MIRABELLA, A ; PROFITA, M ; CHANEZ, P ; BELLIA, V ; MAUTINO, G ; DACCARDI, P ; BOUSQUET, J ; BONSIGNORE, G</creator><creatorcontrib>VIGNOLA, A. M ; RICCOBONO, L ; MIRABELLA, A ; PROFITA, M ; CHANEZ, P ; BELLIA, V ; MAUTINO, G ; DACCARDI, P ; BOUSQUET, J ; BONSIGNORE, G</creatorcontrib><description>Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 control, 31 untreated asthmatic, and 16 chronic bronchitic subjects. We first performed zymographic analysis to identify the profile of MMPs. Zymography revealed a similar MMPs profile in all populations studied and that MMP-9 was the major enzyme released. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and of its inhibitor TIMP-1 and evaluated whether airflow limitation may be associated with an imbalance between these enzymes. MMP-9 and TIMP-1 concentrations were greater in sputum of patients with asthma and chronic bronchitis than in control subjects. The molar ratio between MMP-9 and TIMP-1 was lower in asthmatics and chronic bronchitics than in control subjects, and positively correlated with FEV1 values. In asthma, MMP-9 levels were significantly correlated with the number of macrophages and neutrophils. This study shows that airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1 which may have a role in the pathogenesis of ECM remodeling and airflow obstruction.</description><identifier>ISSN: 1073-449X</identifier><identifier>EISSN: 1535-4970</identifier><identifier>DOI: 10.1164/ajrccm.158.6.9803014</identifier><identifier>PMID: 9847290</identifier><language>eng</language><publisher>New York, NY: American Lung Association</publisher><subject>Adolescent ; Adult ; Aged ; Airway Obstruction - enzymology ; Airway Obstruction - metabolism ; Airway Obstruction - pathology ; Airway Obstruction - physiopathology ; Asthma - enzymology ; Asthma - metabolism ; Asthma - pathology ; Asthma - physiopathology ; Biological and medical sciences ; Bronchitis - enzymology ; Bronchitis - metabolism ; Bronchitis - pathology ; Bronchitis - physiopathology ; Cell Count ; Chronic Disease ; Chronic obstructive pulmonary disease, asthma ; Collagen - metabolism ; Collagenases - analysis ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Extracellular Matrix - metabolism ; Forced Expiratory Volume - physiology ; Homeostasis - physiology ; Humans ; Leukocyte Count ; Macrophages - pathology ; Matrix Metalloproteinase 9 ; Medical sciences ; Middle Aged ; Neutrophils - pathology ; Pneumology ; Protease Inhibitors - analysis ; Pulmonary Ventilation - physiology ; Sodium Dodecyl Sulfate ; Sputum - chemistry ; Sputum - cytology ; Sputum - enzymology ; Surface-Active Agents ; Tissue Inhibitor of Metalloproteinase-1 - analysis</subject><ispartof>American journal of respiratory and critical care medicine, 1998-12, Vol.158 (6), p.1945-1950</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-19eaa5f91dd85e6b13580094a9a642a6bf76a7ed66914e98903d07358a0302683</citedby><cites>FETCH-LOGICAL-c331t-19eaa5f91dd85e6b13580094a9a642a6bf76a7ed66914e98903d07358a0302683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4011,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1624728$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9847290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VIGNOLA, A. M</creatorcontrib><creatorcontrib>RICCOBONO, L</creatorcontrib><creatorcontrib>MIRABELLA, A</creatorcontrib><creatorcontrib>PROFITA, M</creatorcontrib><creatorcontrib>CHANEZ, P</creatorcontrib><creatorcontrib>BELLIA, V</creatorcontrib><creatorcontrib>MAUTINO, G</creatorcontrib><creatorcontrib>DACCARDI, P</creatorcontrib><creatorcontrib>BOUSQUET, J</creatorcontrib><creatorcontrib>BONSIGNORE, G</creatorcontrib><title>Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis</title><title>American journal of respiratory and critical care medicine</title><addtitle>Am J Respir Crit Care Med</addtitle><description>Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 control, 31 untreated asthmatic, and 16 chronic bronchitic subjects. We first performed zymographic analysis to identify the profile of MMPs. Zymography revealed a similar MMPs profile in all populations studied and that MMP-9 was the major enzyme released. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and of its inhibitor TIMP-1 and evaluated whether airflow limitation may be associated with an imbalance between these enzymes. MMP-9 and TIMP-1 concentrations were greater in sputum of patients with asthma and chronic bronchitis than in control subjects. The molar ratio between MMP-9 and TIMP-1 was lower in asthmatics and chronic bronchitics than in control subjects, and positively correlated with FEV1 values. In asthma, MMP-9 levels were significantly correlated with the number of macrophages and neutrophils. This study shows that airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1 which may have a role in the pathogenesis of ECM remodeling and airflow obstruction.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Airway Obstruction - enzymology</subject><subject>Airway Obstruction - metabolism</subject><subject>Airway Obstruction - pathology</subject><subject>Airway Obstruction - physiopathology</subject><subject>Asthma - enzymology</subject><subject>Asthma - metabolism</subject><subject>Asthma - pathology</subject><subject>Asthma - physiopathology</subject><subject>Biological and medical sciences</subject><subject>Bronchitis - enzymology</subject><subject>Bronchitis - metabolism</subject><subject>Bronchitis - pathology</subject><subject>Bronchitis - physiopathology</subject><subject>Cell Count</subject><subject>Chronic Disease</subject><subject>Chronic obstructive pulmonary disease, asthma</subject><subject>Collagen - metabolism</subject><subject>Collagenases - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Extracellular Matrix - metabolism</subject><subject>Forced Expiratory Volume - physiology</subject><subject>Homeostasis - physiology</subject><subject>Humans</subject><subject>Leukocyte Count</subject><subject>Macrophages - pathology</subject><subject>Matrix Metalloproteinase 9</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neutrophils - pathology</subject><subject>Pneumology</subject><subject>Protease Inhibitors - analysis</subject><subject>Pulmonary Ventilation - physiology</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Sputum - chemistry</subject><subject>Sputum - cytology</subject><subject>Sputum - enzymology</subject><subject>Surface-Active Agents</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - analysis</subject><issn>1073-449X</issn><issn>1535-4970</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1KxTAQhYMo_r-BQhbirtekSdNkKeIfCC5UcBemaUojbXNNUsSH8J2N3KIbVzMw35xhzkHohJIVpYJfwFswZlzRSq7ESknCCOVbaJ9WrCq4qsl27knNCs7V6x46iPGNEFpKSnbRrpK8LhXZR19P6znNIx5tgmHw6-CTdRNEW6iL5GKcLXZT7xqXfMC--4ejOEByHhsfgh0g2Yg_XOoxuNAN_gP7JqYwm4xMWQpDTP0IGKYWmz74yRnc5GJ6l88doZ0OhmiPl3qIXm6un6_uiofH2_ury4fCMEZTQZUFqDpF21ZWVjSUVZIQxUGB4CWIpqsF1LYVQlFulVSEtdmJSkI2qRSSHaLzjW7-4322MenRRWOHASbr56jrbBRRjGeQb0ATfIzBdnod3AjhU1Oif1LQmxR0TkELvaSQ104X_bkZbfu7tNie52fLHKKBoQswGRf_tEWZOcm-AVk9lOM</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>VIGNOLA, A. M</creator><creator>RICCOBONO, L</creator><creator>MIRABELLA, A</creator><creator>PROFITA, M</creator><creator>CHANEZ, P</creator><creator>BELLIA, V</creator><creator>MAUTINO, G</creator><creator>DACCARDI, P</creator><creator>BOUSQUET, J</creator><creator>BONSIGNORE, G</creator><general>American Lung Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis</title><author>VIGNOLA, A. M ; RICCOBONO, L ; MIRABELLA, A ; PROFITA, M ; CHANEZ, P ; BELLIA, V ; MAUTINO, G ; DACCARDI, P ; BOUSQUET, J ; BONSIGNORE, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-19eaa5f91dd85e6b13580094a9a642a6bf76a7ed66914e98903d07358a0302683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Airway Obstruction - enzymology</topic><topic>Airway Obstruction - metabolism</topic><topic>Airway Obstruction - pathology</topic><topic>Airway Obstruction - physiopathology</topic><topic>Asthma - enzymology</topic><topic>Asthma - metabolism</topic><topic>Asthma - pathology</topic><topic>Asthma - physiopathology</topic><topic>Biological and medical sciences</topic><topic>Bronchitis - enzymology</topic><topic>Bronchitis - metabolism</topic><topic>Bronchitis - pathology</topic><topic>Bronchitis - physiopathology</topic><topic>Cell Count</topic><topic>Chronic Disease</topic><topic>Chronic obstructive pulmonary disease, asthma</topic><topic>Collagen - metabolism</topic><topic>Collagenases - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Extracellular Matrix - metabolism</topic><topic>Forced Expiratory Volume - physiology</topic><topic>Homeostasis - physiology</topic><topic>Humans</topic><topic>Leukocyte Count</topic><topic>Macrophages - pathology</topic><topic>Matrix Metalloproteinase 9</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neutrophils - pathology</topic><topic>Pneumology</topic><topic>Protease Inhibitors - analysis</topic><topic>Pulmonary Ventilation - physiology</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Sputum - chemistry</topic><topic>Sputum - cytology</topic><topic>Sputum - enzymology</topic><topic>Surface-Active Agents</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VIGNOLA, A. M</creatorcontrib><creatorcontrib>RICCOBONO, L</creatorcontrib><creatorcontrib>MIRABELLA, A</creatorcontrib><creatorcontrib>PROFITA, M</creatorcontrib><creatorcontrib>CHANEZ, P</creatorcontrib><creatorcontrib>BELLIA, V</creatorcontrib><creatorcontrib>MAUTINO, G</creatorcontrib><creatorcontrib>DACCARDI, P</creatorcontrib><creatorcontrib>BOUSQUET, J</creatorcontrib><creatorcontrib>BONSIGNORE, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of respiratory and critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VIGNOLA, A. M</au><au>RICCOBONO, L</au><au>MIRABELLA, A</au><au>PROFITA, M</au><au>CHANEZ, P</au><au>BELLIA, V</au><au>MAUTINO, G</au><au>DACCARDI, P</au><au>BOUSQUET, J</au><au>BONSIGNORE, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis</atitle><jtitle>American journal of respiratory and critical care medicine</jtitle><addtitle>Am J Respir Crit Care Med</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>158</volume><issue>6</issue><spage>1945</spage><epage>1950</epage><pages>1945-1950</pages><issn>1073-449X</issn><eissn>1535-4970</eissn><abstract>Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 control, 31 untreated asthmatic, and 16 chronic bronchitic subjects. We first performed zymographic analysis to identify the profile of MMPs. Zymography revealed a similar MMPs profile in all populations studied and that MMP-9 was the major enzyme released. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and of its inhibitor TIMP-1 and evaluated whether airflow limitation may be associated with an imbalance between these enzymes. MMP-9 and TIMP-1 concentrations were greater in sputum of patients with asthma and chronic bronchitis than in control subjects. The molar ratio between MMP-9 and TIMP-1 was lower in asthmatics and chronic bronchitics than in control subjects, and positively correlated with FEV1 values. In asthma, MMP-9 levels were significantly correlated with the number of macrophages and neutrophils. This study shows that airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1 which may have a role in the pathogenesis of ECM remodeling and airflow obstruction.</abstract><cop>New York, NY</cop><pub>American Lung Association</pub><pmid>9847290</pmid><doi>10.1164/ajrccm.158.6.9803014</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1073-449X |
| ispartof | American journal of respiratory and critical care medicine, 1998-12, Vol.158 (6), p.1945-1950 |
| issn | 1073-449X 1535-4970 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70120934 |
| source | Journals@Ovid Ovid Autoload; MEDLINE; American Thoracic Society (ATS) Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adolescent Adult Aged Airway Obstruction - enzymology Airway Obstruction - metabolism Airway Obstruction - pathology Airway Obstruction - physiopathology Asthma - enzymology Asthma - metabolism Asthma - pathology Asthma - physiopathology Biological and medical sciences Bronchitis - enzymology Bronchitis - metabolism Bronchitis - pathology Bronchitis - physiopathology Cell Count Chronic Disease Chronic obstructive pulmonary disease, asthma Collagen - metabolism Collagenases - analysis Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Extracellular Matrix - metabolism Forced Expiratory Volume - physiology Homeostasis - physiology Humans Leukocyte Count Macrophages - pathology Matrix Metalloproteinase 9 Medical sciences Middle Aged Neutrophils - pathology Pneumology Protease Inhibitors - analysis Pulmonary Ventilation - physiology Sodium Dodecyl Sulfate Sputum - chemistry Sputum - cytology Sputum - enzymology Surface-Active Agents Tissue Inhibitor of Metalloproteinase-1 - analysis |
| title | Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T06%3A05%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sputum%20metalloproteinase-9/tissue%20inhibitor%20of%20metalloproteinase-1%20ratio%20correlates%20with%20airflow%20obstruction%20in%20asthma%20and%20chronic%20bronchitis&rft.jtitle=American%20journal%20of%20respiratory%20and%20critical%20care%20medicine&rft.au=VIGNOLA,%20A.%20M&rft.date=1998-12-01&rft.volume=158&rft.issue=6&rft.spage=1945&rft.epage=1950&rft.pages=1945-1950&rft.issn=1073-449X&rft.eissn=1535-4970&rft_id=info:doi/10.1164/ajrccm.158.6.9803014&rft_dat=%3Cproquest_cross%3E70120934%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70120934&rft_id=info:pmid/9847290&rfr_iscdi=true |