Quantitative Polymerase Chain Reaction Assay for Largemouth Bass Virus
The use of quantitative polymerase chain reaction (QPCR) to test for largemouth bass virus (LMBV) was evaluated during a challenge experiment in which largemouth bass Micropterus salmoides were immersed in the type strain of LMBV. The real‐time PCR and cell culture methods were both used to measure...
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| Veröffentlicht in: | Journal of aquatic animal health 2007-12, Vol.19 (4), p.226-233 |
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| container_title | Journal of aquatic animal health |
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| creator | Getchell, R. G. Groocock, G. H. Schumacher, V. L. Grimmett, S. G. Wooster, G. A. Bowser, P. R. |
| description | The use of quantitative polymerase chain reaction (QPCR) to test for largemouth bass virus (LMBV) was evaluated during a challenge experiment in which largemouth bass Micropterus salmoides were immersed in the type strain of LMBV. The real‐time PCR and cell culture methods were both used to measure LMBV present in the inoculum. Additional samples tested by QPCR included gill, gonad, kidney, liver, mucus, spleen, and swim bladder. A plasmid clone containing a 248‐base pair (bp) fragment of the major capsid protein gene (MCP*) was serially diluted and used as a standard to quantify the number of LMBV DNA copies present in the samples tested. A 62‐bp fragment of DNA located in MCP* was amplified in the real‐time PCR assay. This work has demonstrated the value of the QPCR assay in LMBV surveys. |
| doi_str_mv | 10.1577/H07-009.1 |
| format | Article |
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| ispartof | Journal of aquatic animal health, 2007-12, Vol.19 (4), p.226-233 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Bass - virology Capsid Proteins - genetics Cell Line DNA Primers - chemistry DNA Virus Infections - diagnosis DNA Virus Infections - veterinary DNA Virus Infections - virology Fish Diseases - diagnosis Fish Diseases - virology Freshwater Largemouth bass virus Micropterus salmoides Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Ranavirus - genetics Ranavirus - isolation & purification Reference Values Reproducibility of Results Sensitivity and Specificity |
| title | Quantitative Polymerase Chain Reaction Assay for Largemouth Bass Virus |
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