Selective involvement of caspase-3 in ceramide induced apoptosis in AK-5 tumor cells
Ceramide, a product of sphingomyelin metabolism, is a novel lipid second messenger that mediates diverse cellular functions. The present study demonstrates the activation of caspase-3/CPP-32β, during apoptosis induced by cell permeable exogenous ceramides, in AK-5 tumor, a spontaneously regressing r...
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| Veröffentlicht in: | FEBS letters 1998-11, Vol.439 (1), p.81-84 |
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| container_title | FEBS letters |
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| creator | Anjum, Rana Ali, A.Mubarak Begum, Zareena Vanaja, J. Khar, Ashok |
| description | Ceramide, a product of sphingomyelin metabolism, is a novel lipid second messenger that mediates diverse cellular functions. The present study demonstrates the activation of caspase-3/CPP-32β, during apoptosis induced by cell permeable exogenous ceramides, in AK-5 tumor, a spontaneously regressing rat histiocytoma. The apoptotic events were suppressed by the caspase-3 specific tetrapeptide inhibitor DEVD-CHO but not by the caspase-1 inhibitor YVAD-CMK. In cells overexpressing Bcl-2, a significant decrease in cell death was observed after exogenous addition of ceramides. Furthermore the processing of caspase-3 to its active form upon apoptotic stimulus, and the subsequent cleavage of the substrate PARP, suggested a central role for caspase-3 in the ceramide mediated apoptosis in AK-5 tumor cells. |
| doi_str_mv | 10.1016/S0014-5793(98)01343-X |
| format | Article |
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The present study demonstrates the activation of caspase-3/CPP-32β, during apoptosis induced by cell permeable exogenous ceramides, in AK-5 tumor, a spontaneously regressing rat histiocytoma. The apoptotic events were suppressed by the caspase-3 specific tetrapeptide inhibitor DEVD-CHO but not by the caspase-1 inhibitor YVAD-CMK. In cells overexpressing Bcl-2, a significant decrease in cell death was observed after exogenous addition of ceramides. Furthermore the processing of caspase-3 to its active form upon apoptotic stimulus, and the subsequent cleavage of the substrate PARP, suggested a central role for caspase-3 in the ceramide mediated apoptosis in AK-5 tumor cells.</description><subject>AK-5</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Caspase</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>Ceramide</subject><subject>Ceramides - pharmacology</subject><subject>Enzyme Activation</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Poly(ADP-ribose) Polymerases - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tumor Cells, Cultured</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTtPwzAUhS0EgvL4CUiZEAwBP5LYnlCpgCKQGOjQzXLiG8koqYOdFPXf47QVK0yWfc499-gzQpcE3xJMirsPjEmW5lyyayluMGEZS5cHaEIEZynLCnGIJr-WE3QawieOd0HkMTqWIpNC0AlafEADVW_XkNjV2jVraGHVJ65OKh06HSBlUUgq8Lq1ZjSZoQKT6M51vQs2jOr0Nc2Tfmidj8amCefoqNZNgIv9eYYWT4-L2Tx9e39-mU3f0irjdJlyoWOhsubcFEXNoOAkB1NSKAmVGhtOQOOszqDQOWWUmpznUoAQmJe1LNgZutrFdt59DRB61dowFtArcENQHBPCBBXRmO-MlXcheKhV522r_UYRrEaYagtTjaSUFGoLUy3j3OV-wVC2YH6n9vSiPt_p37aBzf9C1dPjA90qoyDF9nlcdb-LgshrbcGrUFlYRdTWx_9Rxtk_yv4A9JuX1g</recordid><startdate>19981113</startdate><enddate>19981113</enddate><creator>Anjum, Rana</creator><creator>Ali, A.Mubarak</creator><creator>Begum, Zareena</creator><creator>Vanaja, J.</creator><creator>Khar, Ashok</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981113</creationdate><title>Selective involvement of caspase-3 in ceramide induced apoptosis in AK-5 tumor cells</title><author>Anjum, Rana ; Ali, A.Mubarak ; Begum, Zareena ; Vanaja, J. ; Khar, Ashok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472X-78a001bf77d66f3e6715edb2eb129a0d71ea04f4e6a52322d57598e8807bf963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AK-5</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Caspase</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>Ceramide</topic><topic>Ceramides - pharmacology</topic><topic>Enzyme Activation</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Poly(ADP-ribose) Polymerases - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anjum, Rana</creatorcontrib><creatorcontrib>Ali, A.Mubarak</creatorcontrib><creatorcontrib>Begum, Zareena</creatorcontrib><creatorcontrib>Vanaja, J.</creatorcontrib><creatorcontrib>Khar, Ashok</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anjum, Rana</au><au>Ali, A.Mubarak</au><au>Begum, Zareena</au><au>Vanaja, J.</au><au>Khar, Ashok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective involvement of caspase-3 in ceramide induced apoptosis in AK-5 tumor cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1998-11-13</date><risdate>1998</risdate><volume>439</volume><issue>1</issue><spage>81</spage><epage>84</epage><pages>81-84</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Ceramide, a product of sphingomyelin metabolism, is a novel lipid second messenger that mediates diverse cellular functions. 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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1998-11, Vol.439 (1), p.81-84 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | AK-5 Animals Apoptosis Caspase Caspase 3 Caspases - metabolism Ceramide Ceramides - pharmacology Enzyme Activation Peptide Hydrolases - metabolism Poly(ADP-ribose) Polymerases - metabolism Rats Rats, Wistar Tumor Cells, Cultured |
| title | Selective involvement of caspase-3 in ceramide induced apoptosis in AK-5 tumor cells |
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