Profiling glycoprotein N-linked oligosaccharide by capillary electrophoresis

A method for analysis of N‐linked oligosaccharides derived from glycoproteins including sialic acid‐containing species is presented. It is based on the combination of specific chemical and enzymatic conversions coupled with capillary electrophoretic (CE) separation and laser‐induced fluorescence (LI...

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Veröffentlicht in:	Electrophoresis 1998-11, Vol.19 (15), p.2639-2644
Hauptverfasser:	Chen, Fu-Tai A., Evangelista, Ramon A.
Format:	Artikel
Sprache:	eng
Schlagworte:	6-trisulfonic acid 8-Amino-1 8‐Amino‐1,3,6‐trisulfonic acid alpha-Fetoproteins - analysis Amidohydrolases Capillary electrophoresis Carbohydrate Sequence Carbohydrates Catalysis Electrophoresis, Capillary - methods Erythropoietin Erythropoietin - analysis Fetuin Fluorescent Dyes Glucans Glycoproteins - analysis Humans Kallikrein Kallikreins - analysis Laser-induced fluoresence detection Maltose Molecular Sequence Data Oligosaccharides - analysis Oxidation-Reduction Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Pyrenes Ribonuclease B Ribonucleases - analysis Sialidase
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