Isolation of Human Antibodies Against Hepatitis E Virus From Phage Display Library by Immobilized Metal Affinity Chromatography

Objective To isolate human antibodies against hepatitis E virus from phage display library by a new method of panning phage antibody library based on immobilized metal affinity chromatography （1MAC）. Methods Phage antibody library was allowed to mix with hex-His tagged expressed HEV specific antigen...

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Veröffentlicht in:	Biomedical and environmental sciences 2007-12, Vol.20 (6), p.488-494
Hauptverfasser:	Chen, Ying-Wei, Luo, Wen-Xin, Wang, Ming-Qiao, Wang, Jin, Li, Li-Feng, Yuan, Quan, Zhang, Jun, Xia, Ning-Shao
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Viral - chemistry Antibodies, Viral - genetics Antibodies, Viral - immunology Antibodies, Viral - isolation & purification Bacteriophages - genetics Chromatography, Affinity - methods Enzyme-Linked Immunosorbent Assay Hepatitis E virus - immunology HEV抗体 Humans Imidazoles - chemistry Metals Molecular Sequence Data 亲合色谱法抗菌素移动镜头
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