T cell receptor β-chain repertoire in inclusion body myositis
Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8 + cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity...
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| Veröffentlicht in: | Journal of neuroimmunology 1998-11, Vol.91 (1), p.129-134 |
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| creator | Fyhr, Ing-Marie Moslemi, Ali-Reza Lindberg, Christopher Oldfors, Anders |
| description | Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8
+ cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity determining region 3 (CDR3) length distribution of the T cell receptor (TCR). Muscle infiltrating lymphocytes were studied in three IBM patients and compared with peripheral blood lymphocytes (PBL) in two of these patients. The study was performed by reverse transcription polymerase chain reaction (RT-PCR) of RNA extracted from muscle tissue and PBL followed by analysis of fragment length distribution of the CDR3 region in each of 24 different Vβ families. There was a restricted usage of TCR Vβ gene families in muscle infiltrating T cells in all three patients. Some of the TCR Vβ gene families showed oligoclonal expansions but polyclonal patterns were dominating. The CDR3 distribution of most Vβ families differed between muscle infiltrating lymphocytes and PBL indicating that T cells have expanded locally or selectively accumulated in muscle. |
| doi_str_mv | 10.1016/S0165-5728(98)00163-5 |
| format | Article |
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+ cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity determining region 3 (CDR3) length distribution of the T cell receptor (TCR). Muscle infiltrating lymphocytes were studied in three IBM patients and compared with peripheral blood lymphocytes (PBL) in two of these patients. The study was performed by reverse transcription polymerase chain reaction (RT-PCR) of RNA extracted from muscle tissue and PBL followed by analysis of fragment length distribution of the CDR3 region in each of 24 different Vβ families. There was a restricted usage of TCR Vβ gene families in muscle infiltrating T cells in all three patients. Some of the TCR Vβ gene families showed oligoclonal expansions but polyclonal patterns were dominating. The CDR3 distribution of most Vβ families differed between muscle infiltrating lymphocytes and PBL indicating that T cells have expanded locally or selectively accumulated in muscle.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/S0165-5728(98)00163-5</identifier><identifier>PMID: 9846829</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aged ; Aged, 80 and over ; Biopsy ; Complementarity determining region 3 ; DNA Primers ; DNA, Complementary ; Female ; Humans ; Immunoglobulin Variable Region - genetics ; Immunoglobulin Variable Region - immunology ; Inclusion Bodies - immunology ; Inclusion Bodies - pathology ; Inclusion body myositis ; Male ; Middle Aged ; Myositis - immunology ; Myositis - pathology ; Receptors, Antigen, T-Cell, alpha-beta - genetics ; Receptors, Antigen, T-Cell, alpha-beta - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; T cell receptor ; T-Lymphocytes - chemistry ; T-Lymphocytes - immunology</subject><ispartof>Journal of neuroimmunology, 1998-11, Vol.91 (1), p.129-134</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-3bbddbeb5c5a50272eb4dd1a34074349cd1a08c8a8a382f9cbe0412c2a8fc7a3</citedby><cites>FETCH-LOGICAL-c391t-3bbddbeb5c5a50272eb4dd1a34074349cd1a08c8a8a382f9cbe0412c2a8fc7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165572898001635$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9846829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fyhr, Ing-Marie</creatorcontrib><creatorcontrib>Moslemi, Ali-Reza</creatorcontrib><creatorcontrib>Lindberg, Christopher</creatorcontrib><creatorcontrib>Oldfors, Anders</creatorcontrib><title>T cell receptor β-chain repertoire in inclusion body myositis</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8
+ cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity determining region 3 (CDR3) length distribution of the T cell receptor (TCR). Muscle infiltrating lymphocytes were studied in three IBM patients and compared with peripheral blood lymphocytes (PBL) in two of these patients. The study was performed by reverse transcription polymerase chain reaction (RT-PCR) of RNA extracted from muscle tissue and PBL followed by analysis of fragment length distribution of the CDR3 region in each of 24 different Vβ families. There was a restricted usage of TCR Vβ gene families in muscle infiltrating T cells in all three patients. Some of the TCR Vβ gene families showed oligoclonal expansions but polyclonal patterns were dominating. The CDR3 distribution of most Vβ families differed between muscle infiltrating lymphocytes and PBL indicating that T cells have expanded locally or selectively accumulated in muscle.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biopsy</subject><subject>Complementarity determining region 3</subject><subject>DNA Primers</subject><subject>DNA, Complementary</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Immunoglobulin Variable Region - immunology</subject><subject>Inclusion Bodies - immunology</subject><subject>Inclusion Bodies - pathology</subject><subject>Inclusion body myositis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myositis - immunology</subject><subject>Myositis - pathology</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>T cell receptor</subject><subject>T-Lymphocytes - chemistry</subject><subject>T-Lymphocytes - immunology</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUQIMotVY_oTAr0cVonp1ko0jxBQUXdh8ymTsYmZnUZEbob_khfpPpg27dJLm55z44CE0JviGYzG7f0yFyUVB5peQ1ThHLxREaE1nQXHJKjtH4gJyisxg_EyQYVyM0UpLPJFVjdLfMLDRNFsDCqvch-_3J7YdxXfpZQei9C5ClyHW2GaLzXVb6ap21ax9d7-I5OqlNE-Fif0_Q8ulxOX_JF2_Pr_OHRW6ZIn3OyrKqSiiFFUZgWlAoeVURwzgueNrIpjeWVhppmKS1siVgTqilRta2MGyCLndtV8F_DRB73bq42dt04IeoC0zwjCv8L0gKrJQgLIFiB9rgYwxQ61VwrQlrTbDe-NVbv3ojTyupt361SHXT_YChbKE6VO2Fpvz9Lg_JxreDoKN10Fmokkjb68q7fyb8AXEGizI</recordid><startdate>19981102</startdate><enddate>19981102</enddate><creator>Fyhr, Ing-Marie</creator><creator>Moslemi, Ali-Reza</creator><creator>Lindberg, Christopher</creator><creator>Oldfors, Anders</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19981102</creationdate><title>T cell receptor β-chain repertoire in inclusion body myositis</title><author>Fyhr, Ing-Marie ; Moslemi, Ali-Reza ; Lindberg, Christopher ; Oldfors, Anders</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-3bbddbeb5c5a50272eb4dd1a34074349cd1a08c8a8a382f9cbe0412c2a8fc7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biopsy</topic><topic>Complementarity determining region 3</topic><topic>DNA Primers</topic><topic>DNA, Complementary</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Immunoglobulin Variable Region - immunology</topic><topic>Inclusion Bodies - immunology</topic><topic>Inclusion Bodies - pathology</topic><topic>Inclusion body myositis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myositis - immunology</topic><topic>Myositis - pathology</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - genetics</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>T cell receptor</topic><topic>T-Lymphocytes - chemistry</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fyhr, Ing-Marie</creatorcontrib><creatorcontrib>Moslemi, Ali-Reza</creatorcontrib><creatorcontrib>Lindberg, Christopher</creatorcontrib><creatorcontrib>Oldfors, Anders</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fyhr, Ing-Marie</au><au>Moslemi, Ali-Reza</au><au>Lindberg, Christopher</au><au>Oldfors, Anders</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>T cell receptor β-chain repertoire in inclusion body myositis</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1998-11-02</date><risdate>1998</risdate><volume>91</volume><issue>1</issue><spage>129</spage><epage>134</epage><pages>129-134</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8
+ cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity determining region 3 (CDR3) length distribution of the T cell receptor (TCR). Muscle infiltrating lymphocytes were studied in three IBM patients and compared with peripheral blood lymphocytes (PBL) in two of these patients. The study was performed by reverse transcription polymerase chain reaction (RT-PCR) of RNA extracted from muscle tissue and PBL followed by analysis of fragment length distribution of the CDR3 region in each of 24 different Vβ families. There was a restricted usage of TCR Vβ gene families in muscle infiltrating T cells in all three patients. Some of the TCR Vβ gene families showed oligoclonal expansions but polyclonal patterns were dominating. The CDR3 distribution of most Vβ families differed between muscle infiltrating lymphocytes and PBL indicating that T cells have expanded locally or selectively accumulated in muscle.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9846829</pmid><doi>10.1016/S0165-5728(98)00163-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Biopsy Complementarity determining region 3 DNA Primers DNA, Complementary Female Humans Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Inclusion Bodies - immunology Inclusion Bodies - pathology Inclusion body myositis Male Middle Aged Myositis - immunology Myositis - pathology Receptors, Antigen, T-Cell, alpha-beta - genetics Receptors, Antigen, T-Cell, alpha-beta - immunology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis T cell receptor T-Lymphocytes - chemistry T-Lymphocytes - immunology |
| title | T cell receptor β-chain repertoire in inclusion body myositis |
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