High-throughput assays for yeast RNA 5′ triphosphatase (Cet1p)

The 5′ cap on eukaryotic messenger RNA (mRNA) is critical for the stabilization, processing, nuclear transport, and translation of the transcript. Before capping can occur, the γ-phosphate from the 5′ end of newly synthesized RNA must be removed. In Saccharomyces cerevisiae, this reaction is catalyz...

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Veröffentlicht in:	Analytical biochemistry 2008, Vol.372 (1), p.89-95
Hauptverfasser:	Xu, Yiming, Triantafyllou, Ilias, Cable, Michael, Palermo, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Acid Anhydride Hydrolases - metabolism Antifungal drug screening Base Sequence DNA Primers Kinetics Phosphate RNA triphosphatase Saccharomyces cerevisiae - enzymology Scintillation Counting Scintillation proximity assays
Online-Zugang:	Volltext
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