Functional cell surface expression of band 3, the human red blood cell anion exchange protein (AE1), in K562 erythroleukemia cells : Band 3 enhances the cell surface reactivity of Rh antigens

Human K562 erythroleukemia cells were transfected with human band 3 (anion exchanger 1 [AE1]) cDNA, using the pBabe retroviral vector. Stable K562 clones expressing band 3 were isolated by flow cytometry, and surface expression was quantified by immunoblotting. The function of band 3 expressed at th...

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Veröffentlicht in:	Blood 1998-12, Vol.92 (11), p.4428-4438
Hauptverfasser:	BECKMANN, R, SMYTHE, J. S, ANSTEE, D. J, TANNER, M. J. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Anion Exchange Protein 1, Erythrocyte - biosynthesis Anion Exchange Protein 1, Erythrocyte - genetics Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell Membrane - metabolism Cell physiology DNA, Complementary - genetics Flow Cytometry Fundamental and applied biological sciences. Psychology Humans K562 Cells Leukemia, Erythroblastic, Acute - metabolism Molecular and cellular biology Rh-Hr Blood-Group System - metabolism Transfection
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