Discovery and analysis of Bartonella henselae antigens for use in clinical serologic assays
Abstract The antibody response to Bartonella henselae has been studied in a number of mammals; however, the human response needs to be further studied. After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigen...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2008, Vol.60 (1), p.17-23 |
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| creator | McCool, Tera L Hoey, John G Montileone, Francesca Goldenberg, Hannah B Mordechai, Eli Adelson, Martin E |
| description | Abstract The antibody response to Bartonella henselae has been studied in a number of mammals; however, the human response needs to be further studied. After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae . The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed. |
| doi_str_mv | 10.1016/j.diagmicrobio.2007.07.017 |
| format | Article |
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After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae . The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2007.07.017</identifier><identifier>PMID: 17889484</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Angiomatosis, Bacillary - diagnosis ; Angiomatosis, Bacillary - immunology ; Antibodies, Bacterial - blood ; Antigens, Bacterial - analysis ; Antigens, Bacterial - immunology ; Bacterial Proteins - analysis ; Bacterial Proteins - immunology ; Bacteriology ; Bartonella ; Bartonella henselae ; Bartonella henselae - immunology ; Biological and medical sciences ; Diagnostics ; Electrophoresis, Gel, Two-Dimensional ; ELISA ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious Disease ; Internal Medicine ; Microbiology ; Miscellaneous ; Proteomics ; Serologic Tests</subject><ispartof>Diagnostic microbiology and infectious disease, 2008, Vol.60 (1), p.17-23</ispartof><rights>2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-9d82e6de04c9c62f272a4b31222b62c411d3d106d862c8ef5c8d07fb61f582253</citedby><cites>FETCH-LOGICAL-c560t-9d82e6de04c9c62f272a4b31222b62c411d3d106d862c8ef5c8d07fb61f582253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2007.07.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19959204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17889484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCool, Tera L</creatorcontrib><creatorcontrib>Hoey, John G</creatorcontrib><creatorcontrib>Montileone, Francesca</creatorcontrib><creatorcontrib>Goldenberg, Hannah B</creatorcontrib><creatorcontrib>Mordechai, Eli</creatorcontrib><creatorcontrib>Adelson, Martin E</creatorcontrib><title>Discovery and analysis of Bartonella henselae antigens for use in clinical serologic assays</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract The antibody response to Bartonella henselae has been studied in a number of mammals; however, the human response needs to be further studied. After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae . The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed.</description><subject>Angiomatosis, Bacillary - diagnosis</subject><subject>Angiomatosis, Bacillary - immunology</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriology</subject><subject>Bartonella</subject><subject>Bartonella henselae</subject><subject>Bartonella henselae - immunology</subject><subject>Biological and medical sciences</subject><subject>Diagnostics</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>ELISA</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. 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After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae . The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>17889484</pmid><doi>10.1016/j.diagmicrobio.2007.07.017</doi><tpages>7</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2008, Vol.60 (1), p.17-23 |
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| subjects | Angiomatosis, Bacillary - diagnosis Angiomatosis, Bacillary - immunology Antibodies, Bacterial - blood Antigens, Bacterial - analysis Antigens, Bacterial - immunology Bacterial Proteins - analysis Bacterial Proteins - immunology Bacteriology Bartonella Bartonella henselae Bartonella henselae - immunology Biological and medical sciences Diagnostics Electrophoresis, Gel, Two-Dimensional ELISA Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Humans Infectious Disease Internal Medicine Microbiology Miscellaneous Proteomics Serologic Tests |
| title | Discovery and analysis of Bartonella henselae antigens for use in clinical serologic assays |
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