Malaria diagnosis under field conditions in the Venezuelan Amazon
To improve practical, accurate diagnosis of malaria in the Amazon rainforest of Venezuela, two rapid diagnostic tests (RDT) (OptiMAL-IT ® and FalciVax ®) and a laboratory light microscope, used in the field with a battery-operated head lamp as an external light source, were evaluated against the sta...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2008, Vol.102 (1), p.20-24 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
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| creator | Metzger, W.G. Vivas-Martínez, S. Rodriguez, I. Gonçalves, J. Bongard, E. Fanello, C.I. Vivas, L. Magris, M. |
| description | To improve practical, accurate diagnosis of malaria in the Amazon rainforest of Venezuela, two rapid diagnostic tests (RDT) (OptiMAL-IT
® and FalciVax
®) and a laboratory light microscope, used in the field with a battery-operated head lamp as an external light source, were evaluated against the standard laboratory microscope procedure for malaria detection. One hundred and thirty-six Yanomami patients were studied for the presence of malaria parasites. Thirty-three patients (24%) were positive for malaria (
Plasmodium falciparum,
P. vivax,
P. malariae). Twenty-one (64%) of the positive patients had |
| doi_str_mv | 10.1016/j.trstmh.2007.08.007 |
| format | Article |
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® and FalciVax
®) and a laboratory light microscope, used in the field with a battery-operated head lamp as an external light source, were evaluated against the standard laboratory microscope procedure for malaria detection. One hundred and thirty-six Yanomami patients were studied for the presence of malaria parasites. Thirty-three patients (24%) were positive for malaria (
Plasmodium falciparum,
P. vivax,
P. malariae). Twenty-one (64%) of the positive patients had <100 parasites/μl. Both RDTs showed poor sensitivity (24.2% for OptiMAL-IT
® and 36.4% for FalciVax
®) but good specificity (99% both for OptiMAL-IT
® and FalciVax
®). Field and laboratory microscopy showed sensitivities of 94% and 91%, respectively. The κ coefficient was 0.90, indicating a high agreement between field and laboratory microscopy. We conclude that (i) adequate slide reading cannot be substituted by either of the two RDTs in the Venezuelan Amazon and (ii) the use of a light source such as that described above makes slide reading more feasible than hitherto in remote areas without electricity.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/j.trstmh.2007.08.007</identifier><identifier>PMID: 17919672</identifier><identifier>CODEN: TRSTAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Diagnosis ; Diagnostic test ; Human protozoal diseases ; Humans ; Infectious diseases ; Malaria ; Malaria - diagnosis ; Medical sciences ; Microscopy ; Microscopy - methods ; Microscopy - standards ; Parasitemia - diagnosis ; Parasitic diseases ; Plasmodium ; Plasmodium - isolation & purification ; Protozoal diseases ; Quality Control ; Reagent Kits, Diagnostic - parasitology ; Reagent Kits, Diagnostic - standards ; Sensitivity and Specificity ; Venezuela ; Venezuela - epidemiology</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2008, Vol.102 (1), p.20-24</ispartof><rights>2007 Royal Society of Tropical Medicine and Hygiene</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-7cdd7832e9f4491e008afc00cac4e8fb89e8d0503eba22b6bef9eb42c8fd7e853</citedby><cites>FETCH-LOGICAL-c474t-7cdd7832e9f4491e008afc00cac4e8fb89e8d0503eba22b6bef9eb42c8fd7e853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4021,27921,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19998974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17919672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Metzger, W.G.</creatorcontrib><creatorcontrib>Vivas-Martínez, S.</creatorcontrib><creatorcontrib>Rodriguez, I.</creatorcontrib><creatorcontrib>Gonçalves, J.</creatorcontrib><creatorcontrib>Bongard, E.</creatorcontrib><creatorcontrib>Fanello, C.I.</creatorcontrib><creatorcontrib>Vivas, L.</creatorcontrib><creatorcontrib>Magris, M.</creatorcontrib><title>Malaria diagnosis under field conditions in the Venezuelan Amazon</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>To improve practical, accurate diagnosis of malaria in the Amazon rainforest of Venezuela, two rapid diagnostic tests (RDT) (OptiMAL-IT
® and FalciVax
®) and a laboratory light microscope, used in the field with a battery-operated head lamp as an external light source, were evaluated against the standard laboratory microscope procedure for malaria detection. One hundred and thirty-six Yanomami patients were studied for the presence of malaria parasites. Thirty-three patients (24%) were positive for malaria (
Plasmodium falciparum,
P. vivax,
P. malariae). Twenty-one (64%) of the positive patients had <100 parasites/μl. Both RDTs showed poor sensitivity (24.2% for OptiMAL-IT
® and 36.4% for FalciVax
®) but good specificity (99% both for OptiMAL-IT
® and FalciVax
®). Field and laboratory microscopy showed sensitivities of 94% and 91%, respectively. The κ coefficient was 0.90, indicating a high agreement between field and laboratory microscopy. We conclude that (i) adequate slide reading cannot be substituted by either of the two RDTs in the Venezuelan Amazon and (ii) the use of a light source such as that described above makes slide reading more feasible than hitherto in remote areas without electricity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Diagnosis</subject><subject>Diagnostic test</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Malaria</subject><subject>Malaria - diagnosis</subject><subject>Medical sciences</subject><subject>Microscopy</subject><subject>Microscopy - methods</subject><subject>Microscopy - standards</subject><subject>Parasitemia - diagnosis</subject><subject>Parasitic diseases</subject><subject>Plasmodium</subject><subject>Plasmodium - isolation & purification</subject><subject>Protozoal diseases</subject><subject>Quality Control</subject><subject>Reagent Kits, Diagnostic - parasitology</subject><subject>Reagent Kits, Diagnostic - standards</subject><subject>Sensitivity and Specificity</subject><subject>Venezuela</subject><subject>Venezuela - epidemiology</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EotvCGyCUC9ySjp1kbV-QtoXSSq0QUkGIi-XYY-olcVo7QbRPj1dZtTd8-Q_-ZvTPR8gbChUFuj7eVlNM03BTMQBegahyPCMrKrgo6xbq52QFULelZFAfkMOUtgCspa18SQ4ol1SuOVuRzZXudfS6sF7_CmPyqZiDxVg4j70tzBisn_wYUuFDMd1g8R0DPszY61BsBv0whlfkhdN9wtf7PCLfzj5dn56Xl18-X5xuLkvT8GYqubGWi5qhdE0jKQII7QyA0aZB4TohUVjIvbHTjHXrDp3ErmFGOMtRtPUReb_svY3j3YxpUoNPBvvcBMc5KQ4U8hMZbBbQxDGliE7dRj_oeK8oqJ06tVWLOrVTp0CoHHns7X7_3A1on4b2rjLwbg_oZHTvog7GpydOSikkbzJXLpxPE_59_Nfxt1rzmrfq_MdPxfjHrydX1ydqd9iHhcds74_HqJLxGAxaH9FMyo7-_83_AS00nqc</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Metzger, W.G.</creator><creator>Vivas-Martínez, S.</creator><creator>Rodriguez, I.</creator><creator>Gonçalves, J.</creator><creator>Bongard, E.</creator><creator>Fanello, C.I.</creator><creator>Vivas, L.</creator><creator>Magris, M.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Elsevier</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Malaria diagnosis under field conditions in the Venezuelan Amazon</title><author>Metzger, W.G. ; Vivas-Martínez, S. ; Rodriguez, I. ; Gonçalves, J. ; Bongard, E. ; Fanello, C.I. ; Vivas, L. ; Magris, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-7cdd7832e9f4491e008afc00cac4e8fb89e8d0503eba22b6bef9eb42c8fd7e853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Diagnosis</topic><topic>Diagnostic test</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Malaria</topic><topic>Malaria - diagnosis</topic><topic>Medical sciences</topic><topic>Microscopy</topic><topic>Microscopy - methods</topic><topic>Microscopy - standards</topic><topic>Parasitemia - diagnosis</topic><topic>Parasitic diseases</topic><topic>Plasmodium</topic><topic>Plasmodium - isolation & purification</topic><topic>Protozoal diseases</topic><topic>Quality Control</topic><topic>Reagent Kits, Diagnostic - parasitology</topic><topic>Reagent Kits, Diagnostic - standards</topic><topic>Sensitivity and Specificity</topic><topic>Venezuela</topic><topic>Venezuela - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metzger, W.G.</creatorcontrib><creatorcontrib>Vivas-Martínez, S.</creatorcontrib><creatorcontrib>Rodriguez, I.</creatorcontrib><creatorcontrib>Gonçalves, J.</creatorcontrib><creatorcontrib>Bongard, E.</creatorcontrib><creatorcontrib>Fanello, C.I.</creatorcontrib><creatorcontrib>Vivas, L.</creatorcontrib><creatorcontrib>Magris, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metzger, W.G.</au><au>Vivas-Martínez, S.</au><au>Rodriguez, I.</au><au>Gonçalves, J.</au><au>Bongard, E.</au><au>Fanello, C.I.</au><au>Vivas, L.</au><au>Magris, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Malaria diagnosis under field conditions in the Venezuelan Amazon</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2008</date><risdate>2008</risdate><volume>102</volume><issue>1</issue><spage>20</spage><epage>24</epage><pages>20-24</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>To improve practical, accurate diagnosis of malaria in the Amazon rainforest of Venezuela, two rapid diagnostic tests (RDT) (OptiMAL-IT
® and FalciVax
®) and a laboratory light microscope, used in the field with a battery-operated head lamp as an external light source, were evaluated against the standard laboratory microscope procedure for malaria detection. One hundred and thirty-six Yanomami patients were studied for the presence of malaria parasites. Thirty-three patients (24%) were positive for malaria (
Plasmodium falciparum,
P. vivax,
P. malariae). Twenty-one (64%) of the positive patients had <100 parasites/μl. Both RDTs showed poor sensitivity (24.2% for OptiMAL-IT
® and 36.4% for FalciVax
®) but good specificity (99% both for OptiMAL-IT
® and FalciVax
®). Field and laboratory microscopy showed sensitivities of 94% and 91%, respectively. The κ coefficient was 0.90, indicating a high agreement between field and laboratory microscopy. We conclude that (i) adequate slide reading cannot be substituted by either of the two RDTs in the Venezuelan Amazon and (ii) the use of a light source such as that described above makes slide reading more feasible than hitherto in remote areas without electricity.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>17919672</pmid><doi>10.1016/j.trstmh.2007.08.007</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Diagnosis Diagnostic test Human protozoal diseases Humans Infectious diseases Malaria Malaria - diagnosis Medical sciences Microscopy Microscopy - methods Microscopy - standards Parasitemia - diagnosis Parasitic diseases Plasmodium Plasmodium - isolation & purification Protozoal diseases Quality Control Reagent Kits, Diagnostic - parasitology Reagent Kits, Diagnostic - standards Sensitivity and Specificity Venezuela Venezuela - epidemiology |
| title | Malaria diagnosis under field conditions in the Venezuelan Amazon |
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