Comparative analysis of mesenchymal stromal cells from murine bone marrow and amniotic fluid
Background Murine mesenchymal stromal cells (mMSC) are a good model for pre-clinical investigations, and alternative sources of mMSC are subject to intensive experiments. In the present study, we obtained mMSCs from amniotic fluid (AF) and compared their characteristics with mMSCs from bone marrow (...
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| Veröffentlicht in: | Cytotherapy (Oxford, England) England), 2007, Vol.9 (8), p.729-737 |
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| creator | Nadri, S Soleimani, M |
| description | Background Murine mesenchymal stromal cells (mMSC) are a good model for pre-clinical investigations, and alternative sources of mMSC are subject to intensive experiments. In the present study, we obtained mMSCs from amniotic fluid (AF) and compared their characteristics with mMSCs from bone marrow (BM). Methods NMRI mice, 4–6 weeks old, were killed and AF and BM cells collected from those in the second week of pregnancy. MSC were achieved by adhesion to cell culture plastic. Isolated cells were assessed for clonogenic capacity, some surface markers (epitopes) and differentiation potential. Results We achieved AF mMSC more readily than BM mMSC. Differences concerning colony assay and some surface markers of mMSC derived from these two source cells were observed. Most strikingly, AF mMSC showed no adipogenic differentiation capacity, in contrast with BM mMSC. Discussion Our results show that mMSC from AF are an appropriate source for pre-clinical investigation. Furthermore, mMSC from different sources of mice vary in clonogenic capacity, surface markers and differentiation potential. |
| doi_str_mv | 10.1080/14653240701656061 |
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In the present study, we obtained mMSCs from amniotic fluid (AF) and compared their characteristics with mMSCs from bone marrow (BM). Methods NMRI mice, 4–6 weeks old, were killed and AF and BM cells collected from those in the second week of pregnancy. MSC were achieved by adhesion to cell culture plastic. Isolated cells were assessed for clonogenic capacity, some surface markers (epitopes) and differentiation potential. Results We achieved AF mMSC more readily than BM mMSC. Differences concerning colony assay and some surface markers of mMSC derived from these two source cells were observed. Most strikingly, AF mMSC showed no adipogenic differentiation capacity, in contrast with BM mMSC. Discussion Our results show that mMSC from AF are an appropriate source for pre-clinical investigation. Furthermore, mMSC from different sources of mice vary in clonogenic capacity, surface markers and differentiation potential.</description><identifier>ISSN: 1465-3249</identifier><identifier>EISSN: 1477-2566</identifier><identifier>DOI: 10.1080/14653240701656061</identifier><identifier>PMID: 17917881</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adipogenesis ; Advanced Basic Science ; amniotic fluid ; Amniotic Fluid - cytology ; Animals ; Antigens, CD34 ; Antigens, Differentiation ; Bone Marrow Cells - physiology ; Cell Culture Techniques ; Cell Differentiation ; Cell Lineage ; Cell Separation ; Colony-Forming Units Assay ; Female ; GATA2 Transcription Factor - metabolism ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Mice ; murine mesenchymal stromal cells ; Octamer Transcription Factor-3 - metabolism ; Other ; Pregnancy ; Stromal Cells - cytology ; Stromal Cells - physiology</subject><ispartof>Cytotherapy (Oxford, England), 2007, Vol.9 (8), p.729-737</ispartof><rights>International Society for Cellular Therapy</rights><rights>2007 International Society for Cellular Therapy</rights><rights>2007 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-3d721bbeae151cfb0be55d63427ef68b9f56f927446fd1b56dbe3bf9f948bc993</citedby><cites>FETCH-LOGICAL-c458t-3d721bbeae151cfb0be55d63427ef68b9f56f927446fd1b56dbe3bf9f948bc993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/14653240701656061$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/14653240701656061$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27902,27903,27904,61197,61378</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17917881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nadri, S</creatorcontrib><creatorcontrib>Soleimani, M</creatorcontrib><title>Comparative analysis of mesenchymal stromal cells from murine bone marrow and amniotic fluid</title><title>Cytotherapy (Oxford, England)</title><addtitle>Cytotherapy</addtitle><description>Background Murine mesenchymal stromal cells (mMSC) are a good model for pre-clinical investigations, and alternative sources of mMSC are subject to intensive experiments. In the present study, we obtained mMSCs from amniotic fluid (AF) and compared their characteristics with mMSCs from bone marrow (BM). Methods NMRI mice, 4–6 weeks old, were killed and AF and BM cells collected from those in the second week of pregnancy. MSC were achieved by adhesion to cell culture plastic. Isolated cells were assessed for clonogenic capacity, some surface markers (epitopes) and differentiation potential. Results We achieved AF mMSC more readily than BM mMSC. Differences concerning colony assay and some surface markers of mMSC derived from these two source cells were observed. Most strikingly, AF mMSC showed no adipogenic differentiation capacity, in contrast with BM mMSC. Discussion Our results show that mMSC from AF are an appropriate source for pre-clinical investigation. Furthermore, mMSC from different sources of mice vary in clonogenic capacity, surface markers and differentiation potential.</description><subject>Adipogenesis</subject><subject>Advanced Basic Science</subject><subject>amniotic fluid</subject><subject>Amniotic Fluid - cytology</subject><subject>Animals</subject><subject>Antigens, CD34</subject><subject>Antigens, Differentiation</subject><subject>Bone Marrow Cells - physiology</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Cell Separation</subject><subject>Colony-Forming Units Assay</subject><subject>Female</subject><subject>GATA2 Transcription Factor - metabolism</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>murine mesenchymal stromal cells</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Other</subject><subject>Pregnancy</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - physiology</subject><issn>1465-3249</issn><issn>1477-2566</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEURoMozjj6A9xIrdyVJlV5VBAEaXzBgAt1J4Q8buiMSaVNqmbof2-aLhhQmE1uAt_5cjkIvST4DcETfksoZ-NAscCEM445eYQuCRWiHxjnj093zvoWkBfoWa03GA94mthTdEGEJGKayCX6tcvpoItewi10etbxWEPtsu8SVJjt_ph07OpS8mlaiLF2vj26tJYwQ2dyO5IuJd812nU6zSEvwXY-rsE9R0-8jhVebPMK_fz08cfuS3_97fPX3Yfr3lI2Lf3oxECMAQ2EEesNNsCY4yMdBHg-GekZ93IQlHLviGHcGRiNl17SyVgpxyv0-tx7KPnPCnVRKdTTsnqGvFYlMJaSCdaC5By0JddawKtDCW39oyJYnZSq_5Q25tVWvpoE7p7YHLbAu3MgzD6XpPeg47K3uoC6yWtpTuuD9RsNTdBtgKKqDc08uFDALsrl8CD9_h_axjAHq-NvOEK9_1_VQWH1fWuQWLQOOpDxLwUzrJA</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Nadri, S</creator><creator>Soleimani, M</creator><general>Elsevier Inc</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Comparative analysis of mesenchymal stromal cells from murine bone marrow and amniotic fluid</title><author>Nadri, S ; Soleimani, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-3d721bbeae151cfb0be55d63427ef68b9f56f927446fd1b56dbe3bf9f948bc993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adipogenesis</topic><topic>Advanced Basic Science</topic><topic>amniotic fluid</topic><topic>Amniotic Fluid - cytology</topic><topic>Animals</topic><topic>Antigens, CD34</topic><topic>Antigens, Differentiation</topic><topic>Bone Marrow Cells - physiology</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Cell Separation</topic><topic>Colony-Forming Units Assay</topic><topic>Female</topic><topic>GATA2 Transcription Factor - metabolism</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>murine mesenchymal stromal cells</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Other</topic><topic>Pregnancy</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nadri, S</creatorcontrib><creatorcontrib>Soleimani, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytotherapy (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nadri, S</au><au>Soleimani, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of mesenchymal stromal cells from murine bone marrow and amniotic fluid</atitle><jtitle>Cytotherapy (Oxford, England)</jtitle><addtitle>Cytotherapy</addtitle><date>2007</date><risdate>2007</risdate><volume>9</volume><issue>8</issue><spage>729</spage><epage>737</epage><pages>729-737</pages><issn>1465-3249</issn><eissn>1477-2566</eissn><abstract>Background Murine mesenchymal stromal cells (mMSC) are a good model for pre-clinical investigations, and alternative sources of mMSC are subject to intensive experiments. In the present study, we obtained mMSCs from amniotic fluid (AF) and compared their characteristics with mMSCs from bone marrow (BM). Methods NMRI mice, 4–6 weeks old, were killed and AF and BM cells collected from those in the second week of pregnancy. MSC were achieved by adhesion to cell culture plastic. Isolated cells were assessed for clonogenic capacity, some surface markers (epitopes) and differentiation potential. Results We achieved AF mMSC more readily than BM mMSC. Differences concerning colony assay and some surface markers of mMSC derived from these two source cells were observed. Most strikingly, AF mMSC showed no adipogenic differentiation capacity, in contrast with BM mMSC. Discussion Our results show that mMSC from AF are an appropriate source for pre-clinical investigation. 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| subjects | Adipogenesis Advanced Basic Science amniotic fluid Amniotic Fluid - cytology Animals Antigens, CD34 Antigens, Differentiation Bone Marrow Cells - physiology Cell Culture Techniques Cell Differentiation Cell Lineage Cell Separation Colony-Forming Units Assay Female GATA2 Transcription Factor - metabolism Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Mice murine mesenchymal stromal cells Octamer Transcription Factor-3 - metabolism Other Pregnancy Stromal Cells - cytology Stromal Cells - physiology |
| title | Comparative analysis of mesenchymal stromal cells from murine bone marrow and amniotic fluid |
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