Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase
We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells sh...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1998-11, Vol.252 (3), p.764-769 |
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| container_end_page | 769 |
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| container_issue | 3 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Pickering, Clive S. Watkins, Rachel H. Dickson, Alan J. |
| description | We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells show similar sensitivities to cyclic AMP at the level of protein kinase A activation and phosphorylation of cyclic AMP response element binding protein (CREB) and the differential sensitivity must reside at other sites. The consequences of these findings to studies of control phenomena at the transcriptional level is discussed. |
| doi_str_mv | 10.1006/bbrc.1998.9735 |
| format | Article |
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The consequences of these findings to studies of control phenomena at the transcriptional level is discussed.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1998.9735</identifier><identifier>PMID: 9837781</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; CREB ; Cyclic AMP - pharmacology ; Cyclic AMP Response Element-Binding Protein - metabolism ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Enzyme Induction ; Liver - drug effects ; Liver - enzymology ; Liver Neoplasms, Experimental - enzymology ; Male ; protein kinase A ; Rats ; Rats, Sprague-Dawley ; signaling ; transcriptional control ; Tumor Cells, Cultured ; tyrosine aminotransferase ; Tyrosine Transaminase - biosynthesis</subject><ispartof>Biochemical and biophysical research communications, 1998-11, Vol.252 (3), p.764-769</ispartof><rights>1998 Academic Press</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-6b6a2a020e3382c3bbe9a57d48ed06f1bb2c320034cee64485a3907c13924dc03</citedby><cites>FETCH-LOGICAL-c339t-6b6a2a020e3382c3bbe9a57d48ed06f1bb2c320034cee64485a3907c13924dc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1998.9735$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9837781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pickering, Clive S.</creatorcontrib><creatorcontrib>Watkins, Rachel H.</creatorcontrib><creatorcontrib>Dickson, Alan J.</creatorcontrib><title>Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells show similar sensitivities to cyclic AMP at the level of protein kinase A activation and phosphorylation of cyclic AMP response element binding protein (CREB) and the differential sensitivity must reside at other sites. The consequences of these findings to studies of control phenomena at the transcriptional level is discussed.</description><subject>Animals</subject><subject>CREB</subject><subject>Cyclic AMP - pharmacology</subject><subject>Cyclic AMP Response Element-Binding Protein - metabolism</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Enzyme Induction</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver Neoplasms, Experimental - enzymology</subject><subject>Male</subject><subject>protein kinase A</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>signaling</subject><subject>transcriptional control</subject><subject>Tumor Cells, Cultured</subject><subject>tyrosine aminotransferase</subject><subject>Tyrosine Transaminase - biosynthesis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFr3DAQhUVoSbZJr7kFdOrN25Hlta3jskm7hQ0JTQK9CVkeUxVbciTtEv-P_ODK7NJbT4Nmnj5m3iPkmsGSAZRfm8brJROiXoqKr87IgoGALGdQfCALSIosF-zXBfkUwh8AxopSnJNzUfOqqtmCvP9UkT56Myg_0S2OKjo9RQxU2ZZui1NrUHSDfR_orQljr6ZUuw492mhUT5_QBhPNwcSJRkc3k-6Npuv7R5rY8TfSHR6wp66jd2-jxxCMs_PrefIuGIt0PRjrolc2JKgKeEU-dqoP-PlUL8nLt7vnzTbbPXz_sVnvMs25iFnZlCpXkANyXueaNw0KtaraosYWyo41TWrmALzQiGVR1CvFBVSacZEXrQZ-Sb4cuaN3r3sMUQ4m6HSosuj2QVYAydS8TMLlUajTxsFjJ8ejZZKBnGOQcwxyjkHOMaQPNyfyvhmw_Sc_-Z7m9XGO6byDQS-DNmg1tsajjrJ15n_ov8PGmJQ</recordid><startdate>19981127</startdate><enddate>19981127</enddate><creator>Pickering, Clive S.</creator><creator>Watkins, Rachel H.</creator><creator>Dickson, Alan J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981127</creationdate><title>Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase</title><author>Pickering, Clive S. ; Watkins, Rachel H. ; Dickson, Alan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-6b6a2a020e3382c3bbe9a57d48ed06f1bb2c320034cee64485a3907c13924dc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>CREB</topic><topic>Cyclic AMP - pharmacology</topic><topic>Cyclic AMP Response Element-Binding Protein - metabolism</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Enzyme Induction</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Liver Neoplasms, Experimental - enzymology</topic><topic>Male</topic><topic>protein kinase A</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>signaling</topic><topic>transcriptional control</topic><topic>Tumor Cells, Cultured</topic><topic>tyrosine aminotransferase</topic><topic>Tyrosine Transaminase - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pickering, Clive S.</creatorcontrib><creatorcontrib>Watkins, Rachel H.</creatorcontrib><creatorcontrib>Dickson, Alan J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pickering, Clive S.</au><au>Watkins, Rachel H.</au><au>Dickson, Alan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1998-11-27</date><risdate>1998</risdate><volume>252</volume><issue>3</issue><spage>764</spage><epage>769</epage><pages>764-769</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells show similar sensitivities to cyclic AMP at the level of protein kinase A activation and phosphorylation of cyclic AMP response element binding protein (CREB) and the differential sensitivity must reside at other sites. The consequences of these findings to studies of control phenomena at the transcriptional level is discussed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9837781</pmid><doi>10.1006/bbrc.1998.9735</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1998-11, Vol.252 (3), p.764-769 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals CREB Cyclic AMP - pharmacology Cyclic AMP Response Element-Binding Protein - metabolism Cyclic AMP-Dependent Protein Kinases - metabolism Enzyme Induction Liver - drug effects Liver - enzymology Liver Neoplasms, Experimental - enzymology Male protein kinase A Rats Rats, Sprague-Dawley signaling transcriptional control Tumor Cells, Cultured tyrosine aminotransferase Tyrosine Transaminase - biosynthesis |
| title | Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase |
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