Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii

An extracellular glucoamylase produced by Paecilomyces variotii was purified using DEAE-cellulose ion exchange chromatography and Sephadex G-100 gel filtration. The purified protein migrated as a single band in 7% PAGE and 8% SDS-PAGE. The estimated molecular mass was 86.5 kDa (SDS-PAGE). Optima of...

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Veröffentlicht in:	Journal of industrial microbiology & biotechnology 2008, Vol.35 (1), p.17-25
Hauptverfasser:	Michelin, Michele, Ruller, Roberto, Ward, Richard J, Moraes, Luiz Alberto B, Jorge, João A, Terenzi, Héctor F, Polizeli, Maria de Lourdes T. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Amylopectin Amylose Aspergillus oryzae Biochemistry Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biotechnology Cellular biology Cellulose Chromatography Circular Dichroism Culture Media Cyclodextrins Dichroism enzyme activity Enzyme Stability Enzymes Fundamental and applied biological sciences. Psychology Fungi Gel electrophoresis Gel filtration Genetic Engineering Glucan glucan 1,4-alpha-glucosidase Glucan 1,4-alpha-Glucosidase - chemistry Glucan 1,4-alpha-Glucosidase - isolation & purification Glucan 1,4-alpha-Glucosidase - metabolism Glucoamylase Glucose Glycogen Glycogens guavas Hot Temperature Hydrogen-Ion Concentration Inorganic Chemistry Ion exchange Kinetics Laboratories Life Sciences Manganese Mass spectrometry Metal ions Microbiology Molecular Sequence Data Original Paper Paecilomyces Paecilomyces - classification Paecilomyces - enzymology Paecilomyces - growth & development Paecilomyces - physiology Paecilomyces variotii Peptides Proteins Psidium guajava Scientific imaging Sodium Sodium lauryl sulfate Starch Studies Substrates Sucrose Talaromyces Thermal denaturation thermal stability Thin layer chromatography Trehalose β-Cyclodextrin
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