Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina
The neotenic tiger salamander retina is a major model system for the study of retinal physiology and circuitry, yet there are unresolved issues regarding the organization of the photoreceptors and the photoreceptor mosaic. The rod and cone subtypes in the salamander retina were identified using a co...
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| Veröffentlicht in: | Visual neuroscience 1998-11, Vol.15 (6), p.1175-1187 |
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| creator | SHERRY, DAVID M. BUI, DAI D. DEGRIP, WILLEM J. |
| description | The neotenic tiger salamander retina is a major
model system for the study of retinal physiology and circuitry,
yet there are unresolved issues regarding the organization
of the photoreceptors and the photoreceptor mosaic. The
rod and cone subtypes in the salamander retina were identified
using a combination of morphological and immunocytochemical
markers for specific rod and cone opsin epitopes. Because
the visual pigment mechanisms present in the tiger salamander
retina are well characterized and the antibodies employed
in these studies are specific for particular rod and cone
opsin epitopes, we also were able to identify the spectral
class of the various rod and cone subtypes. Two classes
of rods corresponding to the “red” and “green”
rods previously reported in amphibian retinas were identified.
In serial semithin section analyses, rods and cones comprised
62.4 ± 1.4% and 37.6 ± 1.4% of all photoreceptors,
respectively. One rod type comprising 98.0 ± 0.7%
of all rods showed the immunological and morphological
characteristics of “red” rods, which are maximally
sensitive to middle wavelengths. The second rod subtype
comprised 2.0 ± 0.7% of all rods and possessed the
immunological and morphological characteristics of “green”
rods, which are maximally sensitive to short wavelengths.
By morphology four cone types were identified, showing
three distinct immunological signatures. Most cones (84.8
± 1.5% of all cones), including most large single
cones, the accessory and principal members of the double
cone, and some small single cones, showed immunolabeling
by antisera that recognize long wavelength-sensitive cone
opsins. A subpopulation of small single cones (8.4 ±
1.7% of all cones) showed immunolabeling for short wavelength-sensitive
cone opsin. A separate subpopulation of single cones which
included both large and small types (6.8 ± 1.4%
of all cones) was identified as the UV-Cone population
and showed immunolabeling by antibodies that recognize
rod opsin epitopes. Analysis of flatmounted retinas yielded
similar results. All photoreceptor types appeared to be
distributed in all retinal regions. There was no obvious
crystalline organization of the various photoreceptor subtypes
in the photoreceptor mosaic. |
| doi_str_mv | 10.1017/S0952523898156201 |
| format | Article |
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model system for the study of retinal physiology and circuitry,
yet there are unresolved issues regarding the organization
of the photoreceptors and the photoreceptor mosaic. The
rod and cone subtypes in the salamander retina were identified
using a combination of morphological and immunocytochemical
markers for specific rod and cone opsin epitopes. Because
the visual pigment mechanisms present in the tiger salamander
retina are well characterized and the antibodies employed
in these studies are specific for particular rod and cone
opsin epitopes, we also were able to identify the spectral
class of the various rod and cone subtypes. Two classes
of rods corresponding to the “red” and “green”
rods previously reported in amphibian retinas were identified.
In serial semithin section analyses, rods and cones comprised
62.4 ± 1.4% and 37.6 ± 1.4% of all photoreceptors,
respectively. One rod type comprising 98.0 ± 0.7%
of all rods showed the immunological and morphological
characteristics of “red” rods, which are maximally
sensitive to middle wavelengths. The second rod subtype
comprised 2.0 ± 0.7% of all rods and possessed the
immunological and morphological characteristics of “green”
rods, which are maximally sensitive to short wavelengths.
By morphology four cone types were identified, showing
three distinct immunological signatures. Most cones (84.8
± 1.5% of all cones), including most large single
cones, the accessory and principal members of the double
cone, and some small single cones, showed immunolabeling
by antisera that recognize long wavelength-sensitive cone
opsins. A subpopulation of small single cones (8.4 ±
1.7% of all cones) showed immunolabeling for short wavelength-sensitive
cone opsin. A separate subpopulation of single cones which
included both large and small types (6.8 ± 1.4%
of all cones) was identified as the UV-Cone population
and showed immunolabeling by antibodies that recognize
rod opsin epitopes. Analysis of flatmounted retinas yielded
similar results. All photoreceptor types appeared to be
distributed in all retinal regions. There was no obvious
crystalline organization of the various photoreceptor subtypes
in the photoreceptor mosaic.</description><identifier>ISSN: 0952-5238</identifier><identifier>EISSN: 1469-8714</identifier><identifier>DOI: 10.1017/S0952523898156201</identifier><identifier>PMID: 9839981</identifier><language>eng</language><publisher>New York, NY: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Cone ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Opsin ; Photography ; Photoreceptor mosaic ; Retina - cytology ; Retinal Cone Photoreceptor Cells - cytology ; Retinal Rod Photoreceptor Cells - cytology ; Rod ; Salamander ; Urodela - anatomy & histology ; Vertebrates: nervous system and sense organs</subject><ispartof>Visual neuroscience, 1998-11, Vol.15 (6), p.1175-1187</ispartof><rights>1998 Cambridge University Press</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-8826fc65703a9e886c0af629f15500a6f358b82dd4c9af26837e53b3c63540a53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0952523898156201/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1590341$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9839981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHERRY, DAVID M.</creatorcontrib><creatorcontrib>BUI, DAI D.</creatorcontrib><creatorcontrib>DEGRIP, WILLEM J.</creatorcontrib><title>Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina</title><title>Visual neuroscience</title><addtitle>Vis Neurosci</addtitle><description>The neotenic tiger salamander retina is a major
model system for the study of retinal physiology and circuitry,
yet there are unresolved issues regarding the organization
of the photoreceptors and the photoreceptor mosaic. The
rod and cone subtypes in the salamander retina were identified
using a combination of morphological and immunocytochemical
markers for specific rod and cone opsin epitopes. Because
the visual pigment mechanisms present in the tiger salamander
retina are well characterized and the antibodies employed
in these studies are specific for particular rod and cone
opsin epitopes, we also were able to identify the spectral
class of the various rod and cone subtypes. Two classes
of rods corresponding to the “red” and “green”
rods previously reported in amphibian retinas were identified.
In serial semithin section analyses, rods and cones comprised
62.4 ± 1.4% and 37.6 ± 1.4% of all photoreceptors,
respectively. One rod type comprising 98.0 ± 0.7%
of all rods showed the immunological and morphological
characteristics of “red” rods, which are maximally
sensitive to middle wavelengths. The second rod subtype
comprised 2.0 ± 0.7% of all rods and possessed the
immunological and morphological characteristics of “green”
rods, which are maximally sensitive to short wavelengths.
By morphology four cone types were identified, showing
three distinct immunological signatures. Most cones (84.8
± 1.5% of all cones), including most large single
cones, the accessory and principal members of the double
cone, and some small single cones, showed immunolabeling
by antisera that recognize long wavelength-sensitive cone
opsins. A subpopulation of small single cones (8.4 ±
1.7% of all cones) showed immunolabeling for short wavelength-sensitive
cone opsin. A separate subpopulation of single cones which
included both large and small types (6.8 ± 1.4%
of all cones) was identified as the UV-Cone population
and showed immunolabeling by antibodies that recognize
rod opsin epitopes. Analysis of flatmounted retinas yielded
similar results. All photoreceptor types appeared to be
distributed in all retinal regions. There was no obvious
crystalline organization of the various photoreceptor subtypes
in the photoreceptor mosaic.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cone</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Opsin</subject><subject>Photography</subject><subject>Photoreceptor mosaic</subject><subject>Retina - cytology</subject><subject>Retinal Cone Photoreceptor Cells - cytology</subject><subject>Retinal Rod Photoreceptor Cells - cytology</subject><subject>Rod</subject><subject>Salamander</subject><subject>Urodela - anatomy & histology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0952-5238</issn><issn>1469-8714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rFTEYhYNY6m31B7gQZiHuRpPJJJMs5aJtoWA_FIqb8E7mTZs6X00yYP99c71DXQiu3pDnnMPhEPKW0Y-MsubTNdWiEhVXWjEhK8pekA2rpS5Vw-qXZLPD5Y6_Ikcx3lPKOBP8kBxqxXX2bAicdTgm77yF5KexgLErOh9T8O3y52NyxXw3pSmgxTmfIi5tepwxFn4s0h0WI04JR2-L5G8xY-hhyCn5GTD5EV6TAwd9xDfrPSY_vn75vj0tz7-dnG0_n5e2FiKVSlXSWSkaykGjUtJScLLSjglBKUjHhWpV1XW11eAqqXiDgrfcSi5qCoIfkw_73DlMDwvGZAYfLfY95IZLNA2lmnOlspDthTZMMQZ0Zg5-gPBoGDW7Wc0_s2bPuzV8aQfsnh3rjpm_XzlEC70LMFof_wYLTXm9k5V7WV4Yfz9jCL-MbHgjjDy5NKfy6uLm5mJrfmY9X6vC0Abf3aK5n5Yw5h3_U_YJcCGezA</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>SHERRY, DAVID M.</creator><creator>BUI, DAI D.</creator><creator>DEGRIP, WILLEM J.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981101</creationdate><title>Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina</title><author>SHERRY, DAVID M. ; BUI, DAI D. ; DEGRIP, WILLEM J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-8826fc65703a9e886c0af629f15500a6f358b82dd4c9af26837e53b3c63540a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cone</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Opsin</topic><topic>Photography</topic><topic>Photoreceptor mosaic</topic><topic>Retina - cytology</topic><topic>Retinal Cone Photoreceptor Cells - cytology</topic><topic>Retinal Rod Photoreceptor Cells - cytology</topic><topic>Rod</topic><topic>Salamander</topic><topic>Urodela - anatomy & histology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHERRY, DAVID M.</creatorcontrib><creatorcontrib>BUI, DAI D.</creatorcontrib><creatorcontrib>DEGRIP, WILLEM J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Visual neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHERRY, DAVID M.</au><au>BUI, DAI D.</au><au>DEGRIP, WILLEM J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina</atitle><jtitle>Visual neuroscience</jtitle><addtitle>Vis Neurosci</addtitle><date>1998-11-01</date><risdate>1998</risdate><volume>15</volume><issue>6</issue><spage>1175</spage><epage>1187</epage><pages>1175-1187</pages><issn>0952-5238</issn><eissn>1469-8714</eissn><abstract>The neotenic tiger salamander retina is a major
model system for the study of retinal physiology and circuitry,
yet there are unresolved issues regarding the organization
of the photoreceptors and the photoreceptor mosaic. The
rod and cone subtypes in the salamander retina were identified
using a combination of morphological and immunocytochemical
markers for specific rod and cone opsin epitopes. Because
the visual pigment mechanisms present in the tiger salamander
retina are well characterized and the antibodies employed
in these studies are specific for particular rod and cone
opsin epitopes, we also were able to identify the spectral
class of the various rod and cone subtypes. Two classes
of rods corresponding to the “red” and “green”
rods previously reported in amphibian retinas were identified.
In serial semithin section analyses, rods and cones comprised
62.4 ± 1.4% and 37.6 ± 1.4% of all photoreceptors,
respectively. One rod type comprising 98.0 ± 0.7%
of all rods showed the immunological and morphological
characteristics of “red” rods, which are maximally
sensitive to middle wavelengths. The second rod subtype
comprised 2.0 ± 0.7% of all rods and possessed the
immunological and morphological characteristics of “green”
rods, which are maximally sensitive to short wavelengths.
By morphology four cone types were identified, showing
three distinct immunological signatures. Most cones (84.8
± 1.5% of all cones), including most large single
cones, the accessory and principal members of the double
cone, and some small single cones, showed immunolabeling
by antisera that recognize long wavelength-sensitive cone
opsins. A subpopulation of small single cones (8.4 ±
1.7% of all cones) showed immunolabeling for short wavelength-sensitive
cone opsin. A separate subpopulation of single cones which
included both large and small types (6.8 ± 1.4%
of all cones) was identified as the UV-Cone population
and showed immunolabeling by antibodies that recognize
rod opsin epitopes. Analysis of flatmounted retinas yielded
similar results. All photoreceptor types appeared to be
distributed in all retinal regions. There was no obvious
crystalline organization of the various photoreceptor subtypes
in the photoreceptor mosaic.</abstract><cop>New York, NY</cop><pub>Cambridge University Press</pub><pmid>9839981</pmid><doi>10.1017/S0952523898156201</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0952-5238 |
| ispartof | Visual neuroscience, 1998-11, Vol.15 (6), p.1175-1187 |
| issn | 0952-5238 1469-8714 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70093388 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Biological and medical sciences Cone Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Image Processing, Computer-Assisted Immunohistochemistry Opsin Photography Photoreceptor mosaic Retina - cytology Retinal Cone Photoreceptor Cells - cytology Retinal Rod Photoreceptor Cells - cytology Rod Salamander Urodela - anatomy & histology Vertebrates: nervous system and sense organs |
| title | Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina |
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