Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro
Abstract Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel sc...
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| Veröffentlicht in: | Biomaterials 2008-02, Vol.29 (5), p.573-579 |
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| creator | Vashi, Aditya V Keramidaris, Efthimia Abberton, Keren M Morrison, Wayne A Wilson, Jeremy L O’Connor, Andrea J Cooper-White, Justin J Thompson, Erik W |
| description | Abstract Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable. |
| doi_str_mv | 10.1016/j.biomaterials.2007.10.017 |
| format | Article |
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In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2007.10.017</identifier><identifier>PMID: 17980905</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - drug effects ; Adipose tissue engineering ; Advanced Basic Science ; Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Cell culture ; Cell Differentiation - drug effects ; Cells, Cultured ; Copolymer ; Dentistry ; Hydrogel, Polyethylene Glycol Dimethacrylate ; In vitro test ; Lipid ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Poloxamer - pharmacology ; Rats ; Thermally responsive material</subject><ispartof>Biomaterials, 2008-02, Vol.29 (5), p.573-579</ispartof><rights>2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-bb24682bc70822e4d3c23aa86134a49986e44f8624cd9e70ef608d5c379756fa3</citedby><cites>FETCH-LOGICAL-c495t-bb24682bc70822e4d3c23aa86134a49986e44f8624cd9e70ef608d5c379756fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961207008228$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17980905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vashi, Aditya V</creatorcontrib><creatorcontrib>Keramidaris, Efthimia</creatorcontrib><creatorcontrib>Abberton, Keren M</creatorcontrib><creatorcontrib>Morrison, Wayne A</creatorcontrib><creatorcontrib>Wilson, Jeremy L</creatorcontrib><creatorcontrib>O’Connor, Andrea J</creatorcontrib><creatorcontrib>Cooper-White, Justin J</creatorcontrib><creatorcontrib>Thompson, Erik W</creatorcontrib><title>Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose tissue engineering</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Copolymer</subject><subject>Dentistry</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate</subject><subject>In vitro test</subject><subject>Lipid</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Poloxamer - pharmacology</subject><subject>Rats</subject><subject>Thermally responsive material</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhi1ERZfCX0AWB25ZbMfxBwekqh9QqVKRgLPl2JPWSxIvdrJo_30d7UogLu3JsuadeWfmGYTeU7KmhIqPm3Ub4mAnSMH2ec0IkSWwJlS-QCuqpKoaTZqXaEUoZ5UWlJ2i1zlvSPkTzl6hUyq1IkWzQv25D9uYAfvQdZBgnIKdQhxx7HAbR8CDTSn-qXwx24HHA2QY3cN-sD3OEwzYQd9nPOcw3uNv_ZziGBy-riiT-GHvU7yHHocR78KU4ht00pWO4e3xPUM_r69-XHytbu--3Fyc31aO62aq2pZxoVjrJFGMAfe1Y7W1StCaW661EsB5pwTjzmuQBDpBlG9cLbVsRGfrM_ThUHeb4u8Z8mSGkJdG7QhxzkaSMnxNxZPCmhbLhuknhYxw0eiaFeGng9ClmHOCzmxTKDvcG0rMQs9szL_0zEJviRV6Jfnd0WVuB_B_U4-4iuDyIICyvV2AZLILhQf4kMBNxsfwPJ_P_5VxfSjcbP8L9pA3cU7jkkNNZoaY78sdLWdEyuIKEVU_Asyex0g</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Vashi, Aditya V</creator><creator>Keramidaris, Efthimia</creator><creator>Abberton, Keren M</creator><creator>Morrison, Wayne A</creator><creator>Wilson, Jeremy L</creator><creator>O’Connor, Andrea J</creator><creator>Cooper-White, Justin J</creator><creator>Thompson, Erik W</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20080201</creationdate><title>Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro</title><author>Vashi, Aditya V ; 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| subjects | Adipose Tissue - cytology Adipose Tissue - drug effects Adipose tissue engineering Advanced Basic Science Animals Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Cell culture Cell Differentiation - drug effects Cells, Cultured Copolymer Dentistry Hydrogel, Polyethylene Glycol Dimethacrylate In vitro test Lipid Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Poloxamer - pharmacology Rats Thermally responsive material |
| title | Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro |
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