Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements
Initiation of DNA plus-strand synthesis in most reverse-transcribing elements requires primer generation by reverse transcriptase-associated RNase H at one or more template polypurine tracts (PPTs). We have exploited infectious clones of the plant pararetrovirus cauliflower mosaic virus carrying red...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1998-12, Vol.273 (49), p.32568-32575 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 32575 |
|---|---|
| container_issue | 49 |
| container_start_page | 32568 |
| container_title | The Journal of biological chemistry |
| container_volume | 273 |
| creator | Noad, Rob J. Al-Kaff, Nadia S. Turner, David S. Covey, Simon N. |
| description | Initiation of DNA plus-strand synthesis in most reverse-transcribing elements requires primer generation by reverse transcriptase-associated RNase H at one or more template polypurine tracts (PPTs). We have exploited infectious clones of the plant pararetrovirus cauliflower mosaic virus carrying redundant ectopic plus-strand priming elements to study priming in vivo. Ectopic priming generated an additional discontinuity in progeny virion DNA during infection of plants. We found that altering the length of the 13-base pair PPT by ±25% significantly reduced priming efficiency. A short pyrimidine tract 5′ to the PPT, highly conserved among diverse reverse-transcribing elements, was shown to play an important role in PPT recognition in vivo. The predominant DNA plus-strand 5′ end remained 3 nucleotides from the PPT 3′ end in mutant primers that were longer or shorter than the wild-type primer. Use of an ectopic redundant primer to study replication-dependent priming was validated by demonstrating that it could rescue infectivity following destruction of the wild-type priming elements. We propose a model for plant pararetroviral plus-strand priming in which pyrimidines enhance PPT recognition during polymerase-dependent RNase H cleavages, and suggest that fidelity of primer maturation during polymerase-independent cleavages involves PPT length measurement and 3′ end recognition by RNase H. |
| doi_str_mv | 10.1074/jbc.273.49.32568 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70086785</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925819589165</els_id><sourcerecordid>17115554</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-653aedeb6cf218a2dfbd5fbbe2cf0814b8ef805163209f7242f22563c714cfbd3</originalsourceid><addsrcrecordid>eNqFkcGO0zAQhiMEWsrCnQuSD4hbiu3Eic2tKmVBWkEF2xU3y3HGrVdJXGynqPsqvCwOrfa2whdLnm8-eebPstcEzwmuy_d3jZ7TupiXYl5QVvEn2YxgXuQFIz-fZjOMKckFZfx59iKEO5xOKchFdiE4FUIUs-zPYlDdMdiAnEFr1x33o7cDoBuvdMxVCE5bFaFFH78u0LobQx6iV0OL1t72dtgiO6Bbe3BoE21n76cXlTg1RLRWXnmI3h2sVx26BR2dR0vl_XHCvkM7Du0ErlJhb_WDctVBD0MML7NnRnUBXp3vy2zzaXWz_Jxff7v6slxc57qsRMwrVihooam0oYQr2pqmZaZpgGqDOSkbDoZjRqqCYmFqWlJD064KXZNSJ7a4zN6dvHvvfo0Qouxt0NClKcCNQdYY86rm7L8gqQlhjJUJxCdQexeCByP3aTblj5JgOQUnU3AyBSdLIf8Fl1renN1j00P70HBOKtXfnuo7u939th5kY53eQf-Ixign1dbbIDc_SDJgXFfVpPlwqkNa6MGCl0FbGDS0SamjbJ19_I9_ATYGv6w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17115554</pqid></control><display><type>article</type><title>Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Noad, Rob J. ; Al-Kaff, Nadia S. ; Turner, David S. ; Covey, Simon N.</creator><creatorcontrib>Noad, Rob J. ; Al-Kaff, Nadia S. ; Turner, David S. ; Covey, Simon N.</creatorcontrib><description>Initiation of DNA plus-strand synthesis in most reverse-transcribing elements requires primer generation by reverse transcriptase-associated RNase H at one or more template polypurine tracts (PPTs). We have exploited infectious clones of the plant pararetrovirus cauliflower mosaic virus carrying redundant ectopic plus-strand priming elements to study priming in vivo. Ectopic priming generated an additional discontinuity in progeny virion DNA during infection of plants. We found that altering the length of the 13-base pair PPT by ±25% significantly reduced priming efficiency. A short pyrimidine tract 5′ to the PPT, highly conserved among diverse reverse-transcribing elements, was shown to play an important role in PPT recognition in vivo. The predominant DNA plus-strand 5′ end remained 3 nucleotides from the PPT 3′ end in mutant primers that were longer or shorter than the wild-type primer. Use of an ectopic redundant primer to study replication-dependent priming was validated by demonstrating that it could rescue infectivity following destruction of the wild-type priming elements. We propose a model for plant pararetroviral plus-strand priming in which pyrimidines enhance PPT recognition during polymerase-dependent RNase H cleavages, and suggest that fidelity of primer maturation during polymerase-independent cleavages involves PPT length measurement and 3′ end recognition by RNase H.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.273.49.32568</identifier><identifier>PMID: 9829993</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ADN ; Amino Acid Sequence ; Base Sequence ; BRASSICA CAMPESTRIS ; BRASSICA CAMPESTRIS VAR. RAPA ; CAULIFLOWER MOSAIC CAULIMOVIRUS ; Cauliflower mosaic virus ; CAULIMOVIRUS MOSAICO DEL COLIFLOR ; CAULIMOVIRUS MOSAIQUE DU CHOU FLEUR ; DNA ; DNA Primers ; DNA REPLICATION ; DNA, Viral ; EXPERIMENTACION IN VIVO ; EXPERIMENTAL INFECTION ; EXPERIMENTATION IN VIVO ; GENETIC VECTORS ; IN VIVO EXPERIMENTATION ; INFECCION EXPERIMENTAL ; INFECTION EXPERIMENTALE ; INITIATION ; LARGURA ; LENGTH ; LONGUEUR ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Plants - genetics ; Purines - metabolism ; REPLICACION ; REPLICATION ; Retroviridae - genetics ; REVERSE TRANSCRIPTION ; RIBONUCLEASAS ; RIBONUCLEASE ; RIBONUCLEASE H ; RIBONUCLEASES ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; TRANSCRIPCION INVERSA ; TRANSCRIPTION INVERSE ; VECTEUR GENETIQUE ; VECTORES GENETICOS</subject><ispartof>The Journal of biological chemistry, 1998-12, Vol.273 (49), p.32568-32575</ispartof><rights>1998 © 1998 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-653aedeb6cf218a2dfbd5fbbe2cf0814b8ef805163209f7242f22563c714cfbd3</citedby><cites>FETCH-LOGICAL-c469t-653aedeb6cf218a2dfbd5fbbe2cf0814b8ef805163209f7242f22563c714cfbd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9829993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noad, Rob J.</creatorcontrib><creatorcontrib>Al-Kaff, Nadia S.</creatorcontrib><creatorcontrib>Turner, David S.</creatorcontrib><creatorcontrib>Covey, Simon N.</creatorcontrib><title>Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Initiation of DNA plus-strand synthesis in most reverse-transcribing elements requires primer generation by reverse transcriptase-associated RNase H at one or more template polypurine tracts (PPTs). We have exploited infectious clones of the plant pararetrovirus cauliflower mosaic virus carrying redundant ectopic plus-strand priming elements to study priming in vivo. Ectopic priming generated an additional discontinuity in progeny virion DNA during infection of plants. We found that altering the length of the 13-base pair PPT by ±25% significantly reduced priming efficiency. A short pyrimidine tract 5′ to the PPT, highly conserved among diverse reverse-transcribing elements, was shown to play an important role in PPT recognition in vivo. The predominant DNA plus-strand 5′ end remained 3 nucleotides from the PPT 3′ end in mutant primers that were longer or shorter than the wild-type primer. Use of an ectopic redundant primer to study replication-dependent priming was validated by demonstrating that it could rescue infectivity following destruction of the wild-type priming elements. We propose a model for plant pararetroviral plus-strand priming in which pyrimidines enhance PPT recognition during polymerase-dependent RNase H cleavages, and suggest that fidelity of primer maturation during polymerase-independent cleavages involves PPT length measurement and 3′ end recognition by RNase H.</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>BRASSICA CAMPESTRIS</subject><subject>BRASSICA CAMPESTRIS VAR. RAPA</subject><subject>CAULIFLOWER MOSAIC CAULIMOVIRUS</subject><subject>Cauliflower mosaic virus</subject><subject>CAULIMOVIRUS MOSAICO DEL COLIFLOR</subject><subject>CAULIMOVIRUS MOSAIQUE DU CHOU FLEUR</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA REPLICATION</subject><subject>DNA, Viral</subject><subject>EXPERIMENTACION IN VIVO</subject><subject>EXPERIMENTAL INFECTION</subject><subject>EXPERIMENTATION IN VIVO</subject><subject>GENETIC VECTORS</subject><subject>IN VIVO EXPERIMENTATION</subject><subject>INFECCION EXPERIMENTAL</subject><subject>INFECTION EXPERIMENTALE</subject><subject>INITIATION</subject><subject>LARGURA</subject><subject>LENGTH</subject><subject>LONGUEUR</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Plants - genetics</subject><subject>Purines - metabolism</subject><subject>REPLICACION</subject><subject>REPLICATION</subject><subject>Retroviridae - genetics</subject><subject>REVERSE TRANSCRIPTION</subject><subject>RIBONUCLEASAS</subject><subject>RIBONUCLEASE</subject><subject>RIBONUCLEASE H</subject><subject>RIBONUCLEASES</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TRANSCRIPCION INVERSA</subject><subject>TRANSCRIPTION INVERSE</subject><subject>VECTEUR GENETIQUE</subject><subject>VECTORES GENETICOS</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhiMEWsrCnQuSD4hbiu3Eic2tKmVBWkEF2xU3y3HGrVdJXGynqPsqvCwOrfa2whdLnm8-eebPstcEzwmuy_d3jZ7TupiXYl5QVvEn2YxgXuQFIz-fZjOMKckFZfx59iKEO5xOKchFdiE4FUIUs-zPYlDdMdiAnEFr1x33o7cDoBuvdMxVCE5bFaFFH78u0LobQx6iV0OL1t72dtgiO6Bbe3BoE21n76cXlTg1RLRWXnmI3h2sVx26BR2dR0vl_XHCvkM7Du0ErlJhb_WDctVBD0MML7NnRnUBXp3vy2zzaXWz_Jxff7v6slxc57qsRMwrVihooam0oYQr2pqmZaZpgGqDOSkbDoZjRqqCYmFqWlJD064KXZNSJ7a4zN6dvHvvfo0Qouxt0NClKcCNQdYY86rm7L8gqQlhjJUJxCdQexeCByP3aTblj5JgOQUnU3AyBSdLIf8Fl1renN1j00P70HBOKtXfnuo7u939th5kY53eQf-Ixign1dbbIDc_SDJgXFfVpPlwqkNa6MGCl0FbGDS0SamjbJ19_I9_ATYGv6w</recordid><startdate>19981204</startdate><enddate>19981204</enddate><creator>Noad, Rob J.</creator><creator>Al-Kaff, Nadia S.</creator><creator>Turner, David S.</creator><creator>Covey, Simon N.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19981204</creationdate><title>Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements</title><author>Noad, Rob J. ; Al-Kaff, Nadia S. ; Turner, David S. ; Covey, Simon N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-653aedeb6cf218a2dfbd5fbbe2cf0814b8ef805163209f7242f22563c714cfbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>BRASSICA CAMPESTRIS</topic><topic>BRASSICA CAMPESTRIS VAR. RAPA</topic><topic>CAULIFLOWER MOSAIC CAULIMOVIRUS</topic><topic>Cauliflower mosaic virus</topic><topic>CAULIMOVIRUS MOSAICO DEL COLIFLOR</topic><topic>CAULIMOVIRUS MOSAIQUE DU CHOU FLEUR</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA REPLICATION</topic><topic>DNA, Viral</topic><topic>EXPERIMENTACION IN VIVO</topic><topic>EXPERIMENTAL INFECTION</topic><topic>EXPERIMENTATION IN VIVO</topic><topic>GENETIC VECTORS</topic><topic>IN VIVO EXPERIMENTATION</topic><topic>INFECCION EXPERIMENTAL</topic><topic>INFECTION EXPERIMENTALE</topic><topic>INITIATION</topic><topic>LARGURA</topic><topic>LENGTH</topic><topic>LONGUEUR</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Plants - genetics</topic><topic>Purines - metabolism</topic><topic>REPLICACION</topic><topic>REPLICATION</topic><topic>Retroviridae - genetics</topic><topic>REVERSE TRANSCRIPTION</topic><topic>RIBONUCLEASAS</topic><topic>RIBONUCLEASE</topic><topic>RIBONUCLEASE H</topic><topic>RIBONUCLEASES</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TRANSCRIPCION INVERSA</topic><topic>TRANSCRIPTION INVERSE</topic><topic>VECTEUR GENETIQUE</topic><topic>VECTORES GENETICOS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noad, Rob J.</creatorcontrib><creatorcontrib>Al-Kaff, Nadia S.</creatorcontrib><creatorcontrib>Turner, David S.</creatorcontrib><creatorcontrib>Covey, Simon N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noad, Rob J.</au><au>Al-Kaff, Nadia S.</au><au>Turner, David S.</au><au>Covey, Simon N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-12-04</date><risdate>1998</risdate><volume>273</volume><issue>49</issue><spage>32568</spage><epage>32575</epage><pages>32568-32575</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Initiation of DNA plus-strand synthesis in most reverse-transcribing elements requires primer generation by reverse transcriptase-associated RNase H at one or more template polypurine tracts (PPTs). We have exploited infectious clones of the plant pararetrovirus cauliflower mosaic virus carrying redundant ectopic plus-strand priming elements to study priming in vivo. Ectopic priming generated an additional discontinuity in progeny virion DNA during infection of plants. We found that altering the length of the 13-base pair PPT by ±25% significantly reduced priming efficiency. A short pyrimidine tract 5′ to the PPT, highly conserved among diverse reverse-transcribing elements, was shown to play an important role in PPT recognition in vivo. The predominant DNA plus-strand 5′ end remained 3 nucleotides from the PPT 3′ end in mutant primers that were longer or shorter than the wild-type primer. Use of an ectopic redundant primer to study replication-dependent priming was validated by demonstrating that it could rescue infectivity following destruction of the wild-type priming elements. We propose a model for plant pararetroviral plus-strand priming in which pyrimidines enhance PPT recognition during polymerase-dependent RNase H cleavages, and suggest that fidelity of primer maturation during polymerase-independent cleavages involves PPT length measurement and 3′ end recognition by RNase H.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9829993</pmid><doi>10.1074/jbc.273.49.32568</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1998-12, Vol.273 (49), p.32568-32575 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70086785 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ADN Amino Acid Sequence Base Sequence BRASSICA CAMPESTRIS BRASSICA CAMPESTRIS VAR. RAPA CAULIFLOWER MOSAIC CAULIMOVIRUS Cauliflower mosaic virus CAULIMOVIRUS MOSAICO DEL COLIFLOR CAULIMOVIRUS MOSAIQUE DU CHOU FLEUR DNA DNA Primers DNA REPLICATION DNA, Viral EXPERIMENTACION IN VIVO EXPERIMENTAL INFECTION EXPERIMENTATION IN VIVO GENETIC VECTORS IN VIVO EXPERIMENTATION INFECCION EXPERIMENTAL INFECTION EXPERIMENTALE INITIATION LARGURA LENGTH LONGUEUR Molecular Sequence Data NUCLEOTIDE SEQUENCE Plants - genetics Purines - metabolism REPLICACION REPLICATION Retroviridae - genetics REVERSE TRANSCRIPTION RIBONUCLEASAS RIBONUCLEASE RIBONUCLEASE H RIBONUCLEASES SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE TRANSCRIPCION INVERSA TRANSCRIPTION INVERSE VECTEUR GENETIQUE VECTORES GENETICOS |
| title | Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T05%3A07%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20Polypurine%20Tract-associated%20DNA%20Plus-strand%20Priming%20in%20Vivo%20Utilizing%20a%20Plant%20Pararetroviral%20Vector%20Carrying%20Redundant%20Ectopic%20Priming%20Elements&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Noad,%20Rob%20J.&rft.date=1998-12-04&rft.volume=273&rft.issue=49&rft.spage=32568&rft.epage=32575&rft.pages=32568-32575&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.273.49.32568&rft_dat=%3Cproquest_cross%3E17115554%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17115554&rft_id=info:pmid/9829993&rft_els_id=S0021925819589165&rfr_iscdi=true |