Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro
To investigate the characteristics of endothelial cells derived from human cavernous hemangioma in morphology, phenotypes and functions. Endothelial cells were isolated from human hepatic cavernous hemangioma. The morphological, and phenotypical and functional features of these cells were analyzed b...
Gespeichert in:
| Veröffentlicht in: | Zhong hua yi xue za zhi 2007-11, Vol.87 (43), p.3051-3055 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3055 |
|---|---|
| container_issue | 43 |
| container_start_page | 3051 |
| container_title | Zhong hua yi xue za zhi |
| container_volume | 87 |
| creator | Zhang, Wen-Jian Ye, Li-Ya Wu, Lian-Qiu Xin, Yu-Ling Gu, Feng Niu, Ji-Xiao Yang, Zhi-Hua Lou, Jin-Ning |
| description | To investigate the characteristics of endothelial cells derived from human cavernous hemangioma in morphology, phenotypes and functions.
Endothelial cells were isolated from human hepatic cavernous hemangioma. The morphological, and phenotypical and functional features of these cells were analyzed by transmission electron microscopy, fluorescence-activated cell sorter, RT-PCR, zymography, and confocal microscopy. Human liver sinusoidal endothelial cells (LSEC) were used as control.
As compared with the LSEC, abnormally expanded endoplasmic reticulums and similarly arranged cytoplasmic vacuoles were found in the endothelial cells derived from hepatic cavernous hemangioma (HCHEC) by transmission electron microscopy. Flow cytometry showed that expression of alphavbeta3 was significantly increased in the HCHEC. The mRNA of vascular endothelial cell growth factor and angiopoietin 1 were more abundant in HCHEC than that in LSEC. Functional analysis indicated that the HCHEC exhibited strong activated angiogenesis ca |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_70086119</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70086119</sourcerecordid><originalsourceid>FETCH-LOGICAL-p124t-7a79ff3678ae1ef945d9f904f9b3a77a835875701d1512e02f2bda3f83b8246d3</originalsourceid><addsrcrecordid>eNo1UMtKxDAUzUJxhnF-QbJyV8ijbZKlFF8wMBtdSrltbmygbWrSFvx7K46rA-fF4VyRPZOqzERu-I4cU_INy5U0ggl-Q3Zci5LLgu3JR9VBhHbG6NPs20SDozjaMHfYe-hpi32fqN3kFS11MQy0WwYYaYcTbAHawopxDEvamI3_9GEA6ke6-jmGW3LtoE94vOCBvD89vlUv2en8_Fo9nLKJi3zOFCjjnCyVBuToTF5Y4wzLnWkkKAVaFloVinHLCy6QCScaC9Jp2WiRl1YeyP1f7xTD14JprgeffqfDiNu0WjGmS87NZry7GJdmQFtP0Q8Qv-v_Q-QPqsxdfg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70086119</pqid></control><display><type>article</type><title>Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Zhang, Wen-Jian ; Ye, Li-Ya ; Wu, Lian-Qiu ; Xin, Yu-Ling ; Gu, Feng ; Niu, Ji-Xiao ; Yang, Zhi-Hua ; Lou, Jin-Ning</creator><creatorcontrib>Zhang, Wen-Jian ; Ye, Li-Ya ; Wu, Lian-Qiu ; Xin, Yu-Ling ; Gu, Feng ; Niu, Ji-Xiao ; Yang, Zhi-Hua ; Lou, Jin-Ning</creatorcontrib><description>To investigate the characteristics of endothelial cells derived from human cavernous hemangioma in morphology, phenotypes and functions.
Endothelial cells were isolated from human hepatic cavernous hemangioma. The morphological, and phenotypical and functional features of these cells were analyzed by transmission electron microscopy, fluorescence-activated cell sorter, RT-PCR, zymography, and confocal microscopy. Human liver sinusoidal endothelial cells (LSEC) were used as control.
As compared with the LSEC, abnormally expanded endoplasmic reticulums and similarly arranged cytoplasmic vacuoles were found in the endothelial cells derived from hepatic cavernous hemangioma (HCHEC) by transmission electron microscopy. Flow cytometry showed that expression of alphavbeta3 was significantly increased in the HCHEC. The mRNA of vascular endothelial cell growth factor and angiopoietin 1 were more abundant in HCHEC than that in LSEC. Functional analysis indicated that the HCHEC exhibited strong activated angiogenesis ca</description><identifier>ISSN: 0376-2491</identifier><identifier>PMID: 18261350</identifier><language>chi</language><publisher>China</publisher><subject>Angiopoietin-1 - genetics ; Endothelial Cells - metabolism ; Endothelial Cells - pathology ; Endothelial Cells - ultrastructure ; Flow Cytometry ; Hemangioma, Cavernous - genetics ; Hemangioma, Cavernous - metabolism ; Hemangioma, Cavernous - pathology ; Humans ; Integrin alphaVbeta3 - metabolism ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Matrix Metalloproteinase 2 - metabolism ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Zhong hua yi xue za zhi, 2007-11, Vol.87 (43), p.3051-3055</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18261350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Wen-Jian</creatorcontrib><creatorcontrib>Ye, Li-Ya</creatorcontrib><creatorcontrib>Wu, Lian-Qiu</creatorcontrib><creatorcontrib>Xin, Yu-Ling</creatorcontrib><creatorcontrib>Gu, Feng</creatorcontrib><creatorcontrib>Niu, Ji-Xiao</creatorcontrib><creatorcontrib>Yang, Zhi-Hua</creatorcontrib><creatorcontrib>Lou, Jin-Ning</creatorcontrib><title>Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To investigate the characteristics of endothelial cells derived from human cavernous hemangioma in morphology, phenotypes and functions.
Endothelial cells were isolated from human hepatic cavernous hemangioma. The morphological, and phenotypical and functional features of these cells were analyzed by transmission electron microscopy, fluorescence-activated cell sorter, RT-PCR, zymography, and confocal microscopy. Human liver sinusoidal endothelial cells (LSEC) were used as control.
As compared with the LSEC, abnormally expanded endoplasmic reticulums and similarly arranged cytoplasmic vacuoles were found in the endothelial cells derived from hepatic cavernous hemangioma (HCHEC) by transmission electron microscopy. Flow cytometry showed that expression of alphavbeta3 was significantly increased in the HCHEC. The mRNA of vascular endothelial cell growth factor and angiopoietin 1 were more abundant in HCHEC than that in LSEC. Functional analysis indicated that the HCHEC exhibited strong activated angiogenesis ca</description><subject>Angiopoietin-1 - genetics</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelial Cells - pathology</subject><subject>Endothelial Cells - ultrastructure</subject><subject>Flow Cytometry</subject><subject>Hemangioma, Cavernous - genetics</subject><subject>Hemangioma, Cavernous - metabolism</subject><subject>Hemangioma, Cavernous - pathology</subject><subject>Humans</subject><subject>Integrin alphaVbeta3 - metabolism</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron, Transmission</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UMtKxDAUzUJxhnF-QbJyV8ijbZKlFF8wMBtdSrltbmygbWrSFvx7K46rA-fF4VyRPZOqzERu-I4cU_INy5U0ggl-Q3Zci5LLgu3JR9VBhHbG6NPs20SDozjaMHfYe-hpi32fqN3kFS11MQy0WwYYaYcTbAHawopxDEvamI3_9GEA6ke6-jmGW3LtoE94vOCBvD89vlUv2en8_Fo9nLKJi3zOFCjjnCyVBuToTF5Y4wzLnWkkKAVaFloVinHLCy6QCScaC9Jp2WiRl1YeyP1f7xTD14JprgeffqfDiNu0WjGmS87NZry7GJdmQFtP0Q8Qv-v_Q-QPqsxdfg</recordid><startdate>20071120</startdate><enddate>20071120</enddate><creator>Zhang, Wen-Jian</creator><creator>Ye, Li-Ya</creator><creator>Wu, Lian-Qiu</creator><creator>Xin, Yu-Ling</creator><creator>Gu, Feng</creator><creator>Niu, Ji-Xiao</creator><creator>Yang, Zhi-Hua</creator><creator>Lou, Jin-Ning</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20071120</creationdate><title>Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro</title><author>Zhang, Wen-Jian ; Ye, Li-Ya ; Wu, Lian-Qiu ; Xin, Yu-Ling ; Gu, Feng ; Niu, Ji-Xiao ; Yang, Zhi-Hua ; Lou, Jin-Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-7a79ff3678ae1ef945d9f904f9b3a77a835875701d1512e02f2bda3f83b8246d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2007</creationdate><topic>Angiopoietin-1 - genetics</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelial Cells - pathology</topic><topic>Endothelial Cells - ultrastructure</topic><topic>Flow Cytometry</topic><topic>Hemangioma, Cavernous - genetics</topic><topic>Hemangioma, Cavernous - metabolism</topic><topic>Hemangioma, Cavernous - pathology</topic><topic>Humans</topic><topic>Integrin alphaVbeta3 - metabolism</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron, Transmission</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Wen-Jian</creatorcontrib><creatorcontrib>Ye, Li-Ya</creatorcontrib><creatorcontrib>Wu, Lian-Qiu</creatorcontrib><creatorcontrib>Xin, Yu-Ling</creatorcontrib><creatorcontrib>Gu, Feng</creatorcontrib><creatorcontrib>Niu, Ji-Xiao</creatorcontrib><creatorcontrib>Yang, Zhi-Hua</creatorcontrib><creatorcontrib>Lou, Jin-Ning</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Wen-Jian</au><au>Ye, Li-Ya</au><au>Wu, Lian-Qiu</au><au>Xin, Yu-Ling</au><au>Gu, Feng</au><au>Niu, Ji-Xiao</au><au>Yang, Zhi-Hua</au><au>Lou, Jin-Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2007-11-20</date><risdate>2007</risdate><volume>87</volume><issue>43</issue><spage>3051</spage><epage>3055</epage><pages>3051-3055</pages><issn>0376-2491</issn><abstract>To investigate the characteristics of endothelial cells derived from human cavernous hemangioma in morphology, phenotypes and functions.
Endothelial cells were isolated from human hepatic cavernous hemangioma. The morphological, and phenotypical and functional features of these cells were analyzed by transmission electron microscopy, fluorescence-activated cell sorter, RT-PCR, zymography, and confocal microscopy. Human liver sinusoidal endothelial cells (LSEC) were used as control.
As compared with the LSEC, abnormally expanded endoplasmic reticulums and similarly arranged cytoplasmic vacuoles were found in the endothelial cells derived from hepatic cavernous hemangioma (HCHEC) by transmission electron microscopy. Flow cytometry showed that expression of alphavbeta3 was significantly increased in the HCHEC. The mRNA of vascular endothelial cell growth factor and angiopoietin 1 were more abundant in HCHEC than that in LSEC. Functional analysis indicated that the HCHEC exhibited strong activated angiogenesis ca</abstract><cop>China</cop><pmid>18261350</pmid><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0376-2491 |
| ispartof | Zhong hua yi xue za zhi, 2007-11, Vol.87 (43), p.3051-3055 |
| issn | 0376-2491 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_70086119 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Angiopoietin-1 - genetics Endothelial Cells - metabolism Endothelial Cells - pathology Endothelial Cells - ultrastructure Flow Cytometry Hemangioma, Cavernous - genetics Hemangioma, Cavernous - metabolism Hemangioma, Cavernous - pathology Humans Integrin alphaVbeta3 - metabolism Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Matrix Metalloproteinase 2 - metabolism Microscopy, Confocal Microscopy, Electron, Transmission Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Vascular Endothelial Growth Factor A - genetics |
| title | Characteristics of endothelial cells derived from human hepatic cavernous hemangioma in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T20%3A03%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characteristics%20of%20endothelial%20cells%20derived%20from%20human%20hepatic%20cavernous%20hemangioma%20in%20vitro&rft.jtitle=Zhong%20hua%20yi%20xue%20za%20zhi&rft.au=Zhang,%20Wen-Jian&rft.date=2007-11-20&rft.volume=87&rft.issue=43&rft.spage=3051&rft.epage=3055&rft.pages=3051-3055&rft.issn=0376-2491&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E70086119%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70086119&rft_id=info:pmid/18261350&rfr_iscdi=true |