Monitoring of scFv selected by phage display using detection of scFv–pIII fusion proteins in a microtiter scale assay

We describe here a method for the efficient and rapid analysis of antigen binding characteristics of recombinant antibodies (ab) selected by phage display. This novel approach combines the bacterial production of soluble single chain ab (scFv)–pIII fusion proteins on a microtiter scale with the dete...

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Veröffentlicht in:	Journal of immunological methods 1998-11, Vol.220 (1), p.51-58
Hauptverfasser:	Mersmann, Michael, Schmidt, Alexej, Tesar, Michael, Schöneberg, Annette, Welschof, Martin, Kipriyanov, Sergej, Terness, Peter, Little, Melvyn, Pfizenmaier, Klaus, Moosmayer, Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - genetics Antibodies, Bacterial - immunology Antibodies, Bacterial - isolation & purification Antibody Affinity Antigen-Antibody Reactions Bacteriophages Baculoviridae - genetics Biological and medical sciences Capsid Proteins Cell Line DNA-Binding Proteins - genetics Enzyme-Linked Immunosorbent Assay Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic Vectors Humans Immunoglobulin Fragments - genetics Immunoglobulin Fragments - immunology Immunoglobulin Fragments - isolation & purification Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Immunoglobulin Variable Region - isolation & purification Molecular immunology Peptide Library Phage display pIII detection Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Single chain antibodies Spodoptera - cytology Subtraction Technique Surface plasmon resonance analysis Techniques Tetanus toxoid Tetanus Toxoid - immunology Viral Fusion Proteins - genetics
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