Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts
The expression of interferon-tau (IFN-) is essential for bovine embryo survival in the uterus. An evaluation of IFN- production from somatic cell nuclear transfer (NT)-embryo-derived primary trophectoderm cultures in comparison to trophectoderm cultured from parthenogenote (P) and in vitro matured,...
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| Veröffentlicht in: | Molecular reproduction and development 2008-02, Vol.75 (2), p.299-308 |
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| creator | Talbot, Neil C Powell, Anne M Ocon, Olga M Caperna, Thomas J Camp, Mary Garrett, Wesley M Ealy, Alan D |
| description | The expression of interferon-tau (IFN-) is essential for bovine embryo survival in the uterus. An evaluation of IFN- production from somatic cell nuclear transfer (NT)-embryo-derived primary trophectoderm cultures in comparison to trophectoderm cultured from parthenogenote (P) and in vitro matured, fertilized, and cultured (IVP) bovine embryos was performed. In Experiment 1, the success/failure ratio for primary trophectoderm colony formation was similar for IVP and NT blastocysts [IVP = 155/29 (84%); NT 104/25 (81%)], but was decreased (P = .05) for P blastocysts [54/43 (56%)]. Most trophectoderm colonies reached diameters of at least 1 cm within 3-4 weeks, and at this time, 72 hr conditioned cell culture medium was measured for IFN- concentration by antiviral activity assay. The amount of IFN- produced by IVP-outgrowths [4311 IU/mL (n = 155)] was greater (P < .05) than that from NT- [626 IU/mL (n = 104)] and P - [1595 IU/mL (n = 54)] derived trophectoderm. Differential expression of IFN- was confirmed by immunoblotting. In Experiment 2, colony formation was again similar for IVP and NT blastocysts [IVP = 70/5 (93%); NT 67/1 (99%)] and less (P < .05) for P blastocysts [65/27 (70%)]. Analysis of trophectoderm colony size after 23 days in culture showed a similar relationship with P-derived colonies being significantly smaller in comparison to IVP and NT colonies. A differential expression of IFN- was also observed again, but this time as measured over time in culture. Maximal IFN- production was found at day-14 of primary culture and diminished to a minimum by the 23rd day. |
| doi_str_mv | 10.1002/mrd.20741 |
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An evaluation of IFN- production from somatic cell nuclear transfer (NT)-embryo-derived primary trophectoderm cultures in comparison to trophectoderm cultured from parthenogenote (P) and in vitro matured, fertilized, and cultured (IVP) bovine embryos was performed. In Experiment 1, the success/failure ratio for primary trophectoderm colony formation was similar for IVP and NT blastocysts [IVP = 155/29 (84%); NT 104/25 (81%)], but was decreased (P = .05) for P blastocysts [54/43 (56%)]. Most trophectoderm colonies reached diameters of at least 1 cm within 3-4 weeks, and at this time, 72 hr conditioned cell culture medium was measured for IFN- concentration by antiviral activity assay. The amount of IFN- produced by IVP-outgrowths [4311 IU/mL (n = 155)] was greater (P < .05) than that from NT- [626 IU/mL (n = 104)] and P - [1595 IU/mL (n = 54)] derived trophectoderm. Differential expression of IFN- was confirmed by immunoblotting. In Experiment 2, colony formation was again similar for IVP and NT blastocysts [IVP = 70/5 (93%); NT 67/1 (99%)] and less (P < .05) for P blastocysts [65/27 (70%)]. Analysis of trophectoderm colony size after 23 days in culture showed a similar relationship with P-derived colonies being significantly smaller in comparison to IVP and NT colonies. A differential expression of IFN- was also observed again, but this time as measured over time in culture. Maximal IFN- production was found at day-14 of primary culture and diminished to a minimum by the 23rd day.</description><identifier>ISSN: 1098-2795</identifier><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.20741</identifier><identifier>PMID: 17721989</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; blastocyst ; Blastocyst - physiology ; bovine ; Cattle ; cell ; Cell Nucleus - physiology ; Colony-Forming Units Assay ; culture ; differential gene expression ; Ectoderm - physiology ; embryo culture ; embryogenesis ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fertilization ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation, Developmental ; General aspects ; General aspects. Development. Fetal membranes ; in vitro embryo production ; in vitro fertilization ; Interferon Type I - genetics ; interferon-tau ; interferons ; nuclear transplantation ; Organ Culture Techniques ; Parthenogenesis ; parthenogenetic embryos ; Pregnancy Proteins - genetics ; somatic cell nuclear tranfer embryos ; temporal variation ; trophectoderm ; Trophoblasts - cytology ; Trophoblasts - physiology</subject><ispartof>Molecular reproduction and development, 2008-02, Vol.75 (2), p.299-308</ispartof><rights>Published 2007 Wiley‐Liss, Inc.</rights><rights>2008 INIST-CNRS</rights><rights>2007 Wiley-Liss, Inc</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5121-20131e8bea98114b43550ecd6c6d3fd8efec8247285c9c4d2b0ca65253e5aca03</citedby><cites>FETCH-LOGICAL-c5121-20131e8bea98114b43550ecd6c6d3fd8efec8247285c9c4d2b0ca65253e5aca03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.20741$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.20741$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19906767$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17721989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Talbot, Neil C</creatorcontrib><creatorcontrib>Powell, Anne M</creatorcontrib><creatorcontrib>Ocon, Olga M</creatorcontrib><creatorcontrib>Caperna, Thomas J</creatorcontrib><creatorcontrib>Camp, Mary</creatorcontrib><creatorcontrib>Garrett, Wesley M</creatorcontrib><creatorcontrib>Ealy, Alan D</creatorcontrib><creatorcontrib>U.S. Department of Agriculture, Agricultural Research Service</creatorcontrib><creatorcontrib>U.S. Department of Agriculture, Agricultural Research Service</creatorcontrib><title>Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>The expression of interferon-tau (IFN-) is essential for bovine embryo survival in the uterus. An evaluation of IFN- production from somatic cell nuclear transfer (NT)-embryo-derived primary trophectoderm cultures in comparison to trophectoderm cultured from parthenogenote (P) and in vitro matured, fertilized, and cultured (IVP) bovine embryos was performed. In Experiment 1, the success/failure ratio for primary trophectoderm colony formation was similar for IVP and NT blastocysts [IVP = 155/29 (84%); NT 104/25 (81%)], but was decreased (P = .05) for P blastocysts [54/43 (56%)]. Most trophectoderm colonies reached diameters of at least 1 cm within 3-4 weeks, and at this time, 72 hr conditioned cell culture medium was measured for IFN- concentration by antiviral activity assay. The amount of IFN- produced by IVP-outgrowths [4311 IU/mL (n = 155)] was greater (P < .05) than that from NT- [626 IU/mL (n = 104)] and P - [1595 IU/mL (n = 54)] derived trophectoderm. Differential expression of IFN- was confirmed by immunoblotting. In Experiment 2, colony formation was again similar for IVP and NT blastocysts [IVP = 70/5 (93%); NT 67/1 (99%)] and less (P < .05) for P blastocysts [65/27 (70%)]. Analysis of trophectoderm colony size after 23 days in culture showed a similar relationship with P-derived colonies being significantly smaller in comparison to IVP and NT colonies. A differential expression of IFN- was also observed again, but this time as measured over time in culture. Maximal IFN- production was found at day-14 of primary culture and diminished to a minimum by the 23rd day.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>blastocyst</subject><subject>Blastocyst - physiology</subject><subject>bovine</subject><subject>Cattle</subject><subject>cell</subject><subject>Cell Nucleus - physiology</subject><subject>Colony-Forming Units Assay</subject><subject>culture</subject><subject>differential gene expression</subject><subject>Ectoderm - physiology</subject><subject>embryo culture</subject><subject>embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fertilization</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>General aspects</subject><subject>General aspects. Development. Fetal membranes</subject><subject>in vitro embryo production</subject><subject>in vitro fertilization</subject><subject>Interferon Type I - genetics</subject><subject>interferon-tau</subject><subject>interferons</subject><subject>nuclear transplantation</subject><subject>Organ Culture Techniques</subject><subject>Parthenogenesis</subject><subject>parthenogenetic embryos</subject><subject>Pregnancy Proteins - genetics</subject><subject>somatic cell nuclear tranfer embryos</subject><subject>temporal variation</subject><subject>trophectoderm</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - physiology</subject><issn>1098-2795</issn><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0s1uEzEQAOAVAtFSOPAC4AuVkLrt2PtnHyH9ISJtI0jp0XK8s8mW3fXWdtrmIXhnHDZQLoiDNT58M2PNOIpeUzikAOyoteUhgyKlT6JdCoLHrBDZ07_uO9EL524AQAgOz6MdWhSMCi52ox8j0_bK1s50xFTEL5GMO4-2Qmu6eKZW5OSht-hcHUBlTUumtm6VXZOZNf0StTcl2pZcrvzCmnu_dOQYbX2H5aDH36YH5GJ2QFRXkqmyoUFnFuF4JB_NXd2F0CjnjV47715GzyrVOHy1jXvR1enJbPQpnlyejUcfJrHOKKMxA5pQ5HNUglOaztMkywB1meu8TKqSY4Was7RgPNNCpyWbg1Z5xrIEM6UVJHvR_lC3t-Z2hc7LtnYam0Z1aFZOFgA8EWnxX8ggT1NBeYDvB6itcc5iJfthTpKC3CxJhiXJX0sK9s226GreYvkot1sJ4N0WKKdVU1nV6do9OiEgL_LN644Gd183uP53R3n-5fh363jIqJ3Hhz8Zyn6XoV6RyeuLMzlJ6OfT82suR8G_HXyljFSL8E_k1dfN-IHmImEpS34CTsLCrw</recordid><startdate>200802</startdate><enddate>200802</enddate><creator>Talbot, Neil C</creator><creator>Powell, Anne M</creator><creator>Ocon, Olga M</creator><creator>Caperna, Thomas J</creator><creator>Camp, Mary</creator><creator>Garrett, Wesley M</creator><creator>Ealy, Alan D</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200802</creationdate><title>Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts</title><author>Talbot, Neil C ; Powell, Anne M ; Ocon, Olga M ; Caperna, Thomas J ; Camp, Mary ; Garrett, Wesley M ; Ealy, Alan D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5121-20131e8bea98114b43550ecd6c6d3fd8efec8247285c9c4d2b0ca65253e5aca03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>blastocyst</topic><topic>Blastocyst - physiology</topic><topic>bovine</topic><topic>Cattle</topic><topic>cell</topic><topic>Cell Nucleus - physiology</topic><topic>Colony-Forming Units Assay</topic><topic>culture</topic><topic>differential gene expression</topic><topic>Ectoderm - physiology</topic><topic>embryo culture</topic><topic>embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fertilization</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>General aspects</topic><topic>General aspects. Development. Fetal membranes</topic><topic>in vitro embryo production</topic><topic>in vitro fertilization</topic><topic>Interferon Type I - genetics</topic><topic>interferon-tau</topic><topic>interferons</topic><topic>nuclear transplantation</topic><topic>Organ Culture Techniques</topic><topic>Parthenogenesis</topic><topic>parthenogenetic embryos</topic><topic>Pregnancy Proteins - genetics</topic><topic>somatic cell nuclear tranfer embryos</topic><topic>temporal variation</topic><topic>trophectoderm</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Talbot, Neil C</creatorcontrib><creatorcontrib>Powell, Anne M</creatorcontrib><creatorcontrib>Ocon, Olga M</creatorcontrib><creatorcontrib>Caperna, Thomas J</creatorcontrib><creatorcontrib>Camp, Mary</creatorcontrib><creatorcontrib>Garrett, Wesley M</creatorcontrib><creatorcontrib>Ealy, Alan D</creatorcontrib><creatorcontrib>U.S. Department of Agriculture, Agricultural Research Service</creatorcontrib><creatorcontrib>U.S. Department of Agriculture, Agricultural Research Service</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Talbot, Neil C</au><au>Powell, Anne M</au><au>Ocon, Olga M</au><au>Caperna, Thomas J</au><au>Camp, Mary</au><au>Garrett, Wesley M</au><au>Ealy, Alan D</au><aucorp>U.S. Department of Agriculture, Agricultural Research Service</aucorp><aucorp>U.S. Department of Agriculture, Agricultural Research Service</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2008-02</date><risdate>2008</risdate><volume>75</volume><issue>2</issue><spage>299</spage><epage>308</epage><pages>299-308</pages><issn>1098-2795</issn><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>The expression of interferon-tau (IFN-) is essential for bovine embryo survival in the uterus. An evaluation of IFN- production from somatic cell nuclear transfer (NT)-embryo-derived primary trophectoderm cultures in comparison to trophectoderm cultured from parthenogenote (P) and in vitro matured, fertilized, and cultured (IVP) bovine embryos was performed. In Experiment 1, the success/failure ratio for primary trophectoderm colony formation was similar for IVP and NT blastocysts [IVP = 155/29 (84%); NT 104/25 (81%)], but was decreased (P = .05) for P blastocysts [54/43 (56%)]. Most trophectoderm colonies reached diameters of at least 1 cm within 3-4 weeks, and at this time, 72 hr conditioned cell culture medium was measured for IFN- concentration by antiviral activity assay. The amount of IFN- produced by IVP-outgrowths [4311 IU/mL (n = 155)] was greater (P < .05) than that from NT- [626 IU/mL (n = 104)] and P - [1595 IU/mL (n = 54)] derived trophectoderm. Differential expression of IFN- was confirmed by immunoblotting. In Experiment 2, colony formation was again similar for IVP and NT blastocysts [IVP = 70/5 (93%); NT 67/1 (99%)] and less (P < .05) for P blastocysts [65/27 (70%)]. Analysis of trophectoderm colony size after 23 days in culture showed a similar relationship with P-derived colonies being significantly smaller in comparison to IVP and NT colonies. A differential expression of IFN- was also observed again, but this time as measured over time in culture. Maximal IFN- production was found at day-14 of primary culture and diminished to a minimum by the 23rd day.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17721989</pmid><doi>10.1002/mrd.20741</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences blastocyst Blastocyst - physiology bovine Cattle cell Cell Nucleus - physiology Colony-Forming Units Assay culture differential gene expression Ectoderm - physiology embryo culture embryogenesis Embryology: invertebrates and vertebrates. Teratology Female Fertilization Fertilization in Vitro Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Developmental General aspects General aspects. Development. Fetal membranes in vitro embryo production in vitro fertilization Interferon Type I - genetics interferon-tau interferons nuclear transplantation Organ Culture Techniques Parthenogenesis parthenogenetic embryos Pregnancy Proteins - genetics somatic cell nuclear tranfer embryos temporal variation trophectoderm Trophoblasts - cytology Trophoblasts - physiology |
| title | Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts |
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