Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications
A new method is presented for the covalent binding of dextran as an intermediate layer on surface acoustic wave (SAW) devices. For biosensing applications in aqueous media commercially available SAW devices require surface passivation to prevent corrosion of the aluminum device structures in electro...
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| Veröffentlicht in: | Biosensors & bioelectronics 1998-10, Vol.13 (7), p.855-860 |
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| container_title | Biosensors & bioelectronics |
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| creator | Barié, N. Rapp, M. Sigrist, H. Ache, H.J. |
| description | A new method is presented for the covalent binding of dextran as an intermediate layer on surface acoustic wave (SAW) devices. For biosensing applications in aqueous media commercially available SAW devices require surface passivation to prevent corrosion of the aluminum device structures in electrolytes. Thin films of polyimide and parylene revealed exceptional passivation properties. They were used as a base for dextran immobilization. Covalent binding of dextran to polymer-coated surfaces was achieved by photoimmobilization. Aryldiazirine-functionalized bovine serum albumin served as the multifunctional light-activable linking agent (photolinker polymer). Dextran and photolinker polymer were mixed and photobonded to sensor surfaces. Essential photoimmobilization parameters were optimized. The binding of proteins to dextran applying carbodiimide chemistries was exemplified with anti-urease antibodies and the feasibility of specific immunosensing was investigated on SAW sensors connected to a fluid handling system. |
| doi_str_mv | 10.1016/S0956-5663(98)00052-9 |
| format | Article |
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The binding of proteins to dextran applying carbodiimide chemistries was exemplified with anti-urease antibodies and the feasibility of specific immunosensing was investigated on SAW sensors connected to a fluid handling system.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(98)00052-9</identifier><identifier>PMID: 9828382</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Acoustics ; Animals ; Biosensing Techniques ; biosensor ; Cattle ; dextran ; Dextrans ; Light ; mass-sensitivity ; photoimmobilization ; Polymers ; SAW</subject><ispartof>Biosensors & bioelectronics, 1998-10, Vol.13 (7), p.855-860</ispartof><rights>1998 Elsevier Science S.A.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-9aba8cb88f819c108fa6666baaddaad666d749d0d3f144ddc0c895c1e15e375d3</citedby><cites>FETCH-LOGICAL-c391t-9aba8cb88f819c108fa6666baaddaad666d749d0d3f144ddc0c895c1e15e375d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(98)00052-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9828382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barié, N.</creatorcontrib><creatorcontrib>Rapp, M.</creatorcontrib><creatorcontrib>Sigrist, H.</creatorcontrib><creatorcontrib>Ache, H.J.</creatorcontrib><title>Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A new method is presented for the covalent binding of dextran as an intermediate layer on surface acoustic wave (SAW) devices. For biosensing applications in aqueous media commercially available SAW devices require surface passivation to prevent corrosion of the aluminum device structures in electrolytes. Thin films of polyimide and parylene revealed exceptional passivation properties. They were used as a base for dextran immobilization. Covalent binding of dextran to polymer-coated surfaces was achieved by photoimmobilization. Aryldiazirine-functionalized bovine serum albumin served as the multifunctional light-activable linking agent (photolinker polymer). Dextran and photolinker polymer were mixed and photobonded to sensor surfaces. Essential photoimmobilization parameters were optimized. The binding of proteins to dextran applying carbodiimide chemistries was exemplified with anti-urease antibodies and the feasibility of specific immunosensing was investigated on SAW sensors connected to a fluid handling system.</description><subject>Acoustics</subject><subject>Animals</subject><subject>Biosensing Techniques</subject><subject>biosensor</subject><subject>Cattle</subject><subject>dextran</subject><subject>Dextrans</subject><subject>Light</subject><subject>mass-sensitivity</subject><subject>photoimmobilization</subject><subject>Polymers</subject><subject>SAW</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rHSEUhiW0JDcfPyHgqrSLaXUcZ3RVyqVJC4Eu2q7F0TOpjaNT9YbervvD4_0g2wjiwfOc98D7InRNyXtKaP_hO5G8b3jfs7dSvCOE8LaRJ2hFxcCarmX8FVo9I2foPOffFRqoJKfoVIpWMNGu0P91fNQeQsHLr1iid-EBUjODdbqAxW6e4-i8-6eLiwHHCeuAXSiQjgi28Lek-un1FhIuES_Rb-eqYeJeIW_SpA1kPMWERxczhOzCPdbL4p3Zy-ZL9HrSPsPV8b1AP28-_1h_ae6-3X5df7prDJO0NFKPWphRiElQaSgRk-7rGbW2tt5a2qGTllg20a6z1hAjJDcUKAc2cMsu0JuD7pLinw3komaXDXivA8RNVgMhgjLevQi2QzfUVTuQH0CTYs4JJrUkN-u0VZSoXUxqH5PaZaCkUPuYlKxz18cFm7E6-Tx1zKX2Px76UO14dJBUNg6CqaYnMEXZ6F7Y8ARq7aa6</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Barié, N.</creator><creator>Rapp, M.</creator><creator>Sigrist, H.</creator><creator>Ache, H.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications</title><author>Barié, N. ; Rapp, M. ; Sigrist, H. ; Ache, H.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-9aba8cb88f819c108fa6666baaddaad666d749d0d3f144ddc0c895c1e15e375d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acoustics</topic><topic>Animals</topic><topic>Biosensing Techniques</topic><topic>biosensor</topic><topic>Cattle</topic><topic>dextran</topic><topic>Dextrans</topic><topic>Light</topic><topic>mass-sensitivity</topic><topic>photoimmobilization</topic><topic>Polymers</topic><topic>SAW</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barié, N.</creatorcontrib><creatorcontrib>Rapp, M.</creatorcontrib><creatorcontrib>Sigrist, H.</creatorcontrib><creatorcontrib>Ache, H.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barié, N.</au><au>Rapp, M.</au><au>Sigrist, H.</au><au>Ache, H.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>13</volume><issue>7</issue><spage>855</spage><epage>860</epage><pages>855-860</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A new method is presented for the covalent binding of dextran as an intermediate layer on surface acoustic wave (SAW) devices. 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| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 1998-10, Vol.13 (7), p.855-860 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acoustics Animals Biosensing Techniques biosensor Cattle dextran Dextrans Light mass-sensitivity photoimmobilization Polymers SAW |
| title | Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications |
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