Transposition of the Drosophila element mariner into the chicken germ line

The ability of the Drosophila transposable element mariner to transpose in the chicken was tested using a plasmid carrying an active mariner element injected into chick zygotes. Surviving embryos and chicks were analyzed for presence of mariner. Analysis of embryos that survived for at least 12 days...

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Veröffentlicht in:	Nature biotechnology 1998-11, Vol.16 (11), p.1050-1053
Hauptverfasser:	Sherman, A. (Roslin Institute, Roslin, UK.), Dawson, A, Mather, C, Gilhooley, H, Li, Y, Mitchell, R, Finnegan, D, Sang, H
Format:	Artikel
Sprache:	eng
Schlagworte:	ANIMAL TRANSGENIQUE ANIMALES TRANSGENICOS Animals Animals, Genetically Modified Base Sequence Biological and medical sciences Biotechnology Chick Embryo CHICKENS Chickens - genetics CIGOTOS DNA Primers - genetics DNA Transposable Elements - genetics DNA-Binding Proteins DROSOPHILA Drosophila - genetics EMBRYOS Fundamental and applied biological sciences. Psychology GENE GENES Genes, Insect Genetic engineering GENETIC INHERITANCE Genetic technics Genetic Vectors Germ-Line Mutation HEREDITE HERENCIA GENETICA Methods. Procedures. Technologies POLLO POULET TRANSGENIC ANIMALS Transgenic animals and transgenic plants TRANSPOSABLE ELEMENTS Transposases - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons ZYGOTE ZYGOTES
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