Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line
The chicken fibroblast cell line C32 has been transfected with the chicken homolog (Ch-IRF-1) of the mammalian transcription factor IRF-1. Stable transfectants were generated, constitutively overexpressing Ch-IRF-1 mRNA and protein. Cells overexpressing Ch-IRF-1 showed enhanced constitutive expressi...
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| Veröffentlicht in: | Gene 1998-11, Vol.222 (2), p.269-278 |
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| creator | Zoeller, B Popp, M Walter, A Redmann-Müller, I Lodemann, E Jungwirth, C |
| description | The chicken fibroblast cell line C32 has been transfected with the chicken homolog (Ch-IRF-1) of the mammalian transcription factor IRF-1. Stable transfectants were generated, constitutively overexpressing Ch-IRF-1 mRNA and protein. Cells overexpressing Ch-IRF-1 showed enhanced constitutive expression of MHC class I (B-F,
β-microglobulin) antigens. With increasing number of passages cells with normal B-F IV surface antigen expression accumulated. In the revertants, the amount of Ch-IRF-1 mRNA was reduced. Overexpression of Ch-IRF-1 had no effect on the constitutive expression and the induction by chicken interferon type-I and type-II (Ch-IFN) of guanylate-binding protein (GBP). Susceptibility to vesicular stomatitis virus, sindbis virus, Newcastle disease virus and vaccinia virus was not altered by overexpression of Ch-IRF-1. An antiviral state could be induced against all viruses tested by similar amounts of Ch-IFN type I in clone 20-18 expressing Ch-IRF-1 and cells transfected with empty vector. |
| doi_str_mv | 10.1016/S0378-1119(98)00504-6 |
| format | Article |
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β-microglobulin) antigens. With increasing number of passages cells with normal B-F IV surface antigen expression accumulated. In the revertants, the amount of Ch-IRF-1 mRNA was reduced. Overexpression of Ch-IRF-1 had no effect on the constitutive expression and the induction by chicken interferon type-I and type-II (Ch-IFN) of guanylate-binding protein (GBP). Susceptibility to vesicular stomatitis virus, sindbis virus, Newcastle disease virus and vaccinia virus was not altered by overexpression of Ch-IRF-1. An antiviral state could be induced against all viruses tested by similar amounts of Ch-IFN type I in clone 20-18 expressing Ch-IRF-1 and cells transfected with empty vector.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00504-6</identifier><identifier>PMID: 9831662</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antiviral effect ; b-f antigen ; beta-microglubulin ; binding proteins ; Carrier Proteins - genetics ; Cell Line ; Chickens ; disease resistance ; DNA, Complementary - genetics ; DNA-Binding Proteins - genetics ; fibroblasts ; Gene Expression ; gene overexpression ; Guanosine Monophosphate - metabolism ; gyanylate-binding protein ; histocompatibility antigens ; Histocompatibility Antigens Class I - genetics ; IFN-signal transduction ; Interferon Regulatory Factor-1 ; interferons ; major histocompatibility complex ; Mammals ; messenger RNA ; Newcastle disease virus ; Newcastle disease virus - immunology ; Phosphoproteins - genetics ; Sindbis virus ; Sindbis Virus - immunology ; transcription factors ; Transcriptional activator ; transcriptional activators ; Transfection ; Vaccinia virus ; Vaccinia virus - immunology ; Vesicular stomatitis Indiana virus - immunology ; Vesicular stomatitis virus ; Vesiculovirus</subject><ispartof>Gene, 1998-11, Vol.222 (2), p.269-278</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-3c2b56697b2c1a599d2ef1a51288c3120974aa348a0a42151e4963659aad7cb43</citedby><cites>FETCH-LOGICAL-c415t-3c2b56697b2c1a599d2ef1a51288c3120974aa348a0a42151e4963659aad7cb43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111998005046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9831662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zoeller, B</creatorcontrib><creatorcontrib>Popp, M</creatorcontrib><creatorcontrib>Walter, A</creatorcontrib><creatorcontrib>Redmann-Müller, I</creatorcontrib><creatorcontrib>Lodemann, E</creatorcontrib><creatorcontrib>Jungwirth, C</creatorcontrib><title>Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line</title><title>Gene</title><addtitle>Gene</addtitle><description>The chicken fibroblast cell line C32 has been transfected with the chicken homolog (Ch-IRF-1) of the mammalian transcription factor IRF-1. Stable transfectants were generated, constitutively overexpressing Ch-IRF-1 mRNA and protein. Cells overexpressing Ch-IRF-1 showed enhanced constitutive expression of MHC class I (B-F,
β-microglobulin) antigens. With increasing number of passages cells with normal B-F IV surface antigen expression accumulated. In the revertants, the amount of Ch-IRF-1 mRNA was reduced. Overexpression of Ch-IRF-1 had no effect on the constitutive expression and the induction by chicken interferon type-I and type-II (Ch-IFN) of guanylate-binding protein (GBP). Susceptibility to vesicular stomatitis virus, sindbis virus, Newcastle disease virus and vaccinia virus was not altered by overexpression of Ch-IRF-1. An antiviral state could be induced against all viruses tested by similar amounts of Ch-IFN type I in clone 20-18 expressing Ch-IRF-1 and cells transfected with empty vector.</description><subject>Animals</subject><subject>Antiviral effect</subject><subject>b-f antigen</subject><subject>beta-microglubulin</subject><subject>binding proteins</subject><subject>Carrier Proteins - genetics</subject><subject>Cell Line</subject><subject>Chickens</subject><subject>disease resistance</subject><subject>DNA, Complementary - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>fibroblasts</subject><subject>Gene Expression</subject><subject>gene overexpression</subject><subject>Guanosine Monophosphate - metabolism</subject><subject>gyanylate-binding protein</subject><subject>histocompatibility antigens</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>IFN-signal transduction</subject><subject>Interferon Regulatory Factor-1</subject><subject>interferons</subject><subject>major histocompatibility complex</subject><subject>Mammals</subject><subject>messenger RNA</subject><subject>Newcastle disease virus</subject><subject>Newcastle disease virus - immunology</subject><subject>Phosphoproteins - genetics</subject><subject>Sindbis virus</subject><subject>Sindbis Virus - immunology</subject><subject>transcription factors</subject><subject>Transcriptional activator</subject><subject>transcriptional activators</subject><subject>Transfection</subject><subject>Vaccinia virus</subject><subject>Vaccinia virus - immunology</subject><subject>Vesicular stomatitis Indiana virus - immunology</subject><subject>Vesicular stomatitis virus</subject><subject>Vesiculovirus</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQjRCoLIWfUOETag8Bf8SOfULVitKViipRerYcZ7I1ZO3Fdlb0P_KjcLqrlTjVl7Fm3nvzNK-qzgj-SDARn-4wa2VNCFHnSl5gzHFTixfVgshW1Rgz-bJaHCGvqzcp_cTlcU5PqhMlGRGCLqq_tzuI8GcbISUXPAoDsg_O_gKPnM8QB4ilG2E9jSaH-IgGY0utCTpfPtSr71c1uSjIfrKQkA0-ZZen7HaA_hf9dr1EdjQpoRUyPrs1-IS6KaM-FKIPeSaXZWjn4pTKwuRSNt4CygEZtIW4MR58ProbXBdDVxRLC8YRjc7D2-rVYMYE7w71tLq_-vJjeV3f3H5dLS9vatsQnmtmaceFUG1HLTFcqZ7CUD6ESmkZoVi1jTGskQabhhJOoFGCCa6M6VvbNey0-rDX3cbwe4KU9cal2UWxGKakW4zbtsXkWSBpCROSiQLke6CNIaUIg95GtzHxUROs57j1U9x6zlIrqZ_i1jPv7LBg6jbQH1mHfMv8_X4-mKDNOrqk7-9ocYapYpSrWeHzHgHlYDsHUSfroBy-dxFs1n1wz3j4BzvBxjA</recordid><startdate>19981119</startdate><enddate>19981119</enddate><creator>Zoeller, B</creator><creator>Popp, M</creator><creator>Walter, A</creator><creator>Redmann-Müller, I</creator><creator>Lodemann, E</creator><creator>Jungwirth, C</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19981119</creationdate><title>Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line</title><author>Zoeller, B ; Popp, M ; Walter, A ; Redmann-Müller, I ; Lodemann, E ; Jungwirth, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-3c2b56697b2c1a599d2ef1a51288c3120974aa348a0a42151e4963659aad7cb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antiviral effect</topic><topic>b-f antigen</topic><topic>beta-microglubulin</topic><topic>binding proteins</topic><topic>Carrier Proteins - genetics</topic><topic>Cell Line</topic><topic>Chickens</topic><topic>disease resistance</topic><topic>DNA, Complementary - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>fibroblasts</topic><topic>Gene Expression</topic><topic>gene overexpression</topic><topic>Guanosine Monophosphate - metabolism</topic><topic>gyanylate-binding protein</topic><topic>histocompatibility antigens</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>IFN-signal transduction</topic><topic>Interferon Regulatory Factor-1</topic><topic>interferons</topic><topic>major histocompatibility complex</topic><topic>Mammals</topic><topic>messenger RNA</topic><topic>Newcastle disease virus</topic><topic>Newcastle disease virus - immunology</topic><topic>Phosphoproteins - genetics</topic><topic>Sindbis virus</topic><topic>Sindbis Virus - immunology</topic><topic>transcription factors</topic><topic>Transcriptional activator</topic><topic>transcriptional activators</topic><topic>Transfection</topic><topic>Vaccinia virus</topic><topic>Vaccinia virus - immunology</topic><topic>Vesicular stomatitis Indiana virus - immunology</topic><topic>Vesicular stomatitis virus</topic><topic>Vesiculovirus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zoeller, B</creatorcontrib><creatorcontrib>Popp, M</creatorcontrib><creatorcontrib>Walter, A</creatorcontrib><creatorcontrib>Redmann-Müller, I</creatorcontrib><creatorcontrib>Lodemann, E</creatorcontrib><creatorcontrib>Jungwirth, C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zoeller, B</au><au>Popp, M</au><au>Walter, A</au><au>Redmann-Müller, I</au><au>Lodemann, E</au><au>Jungwirth, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1998-11-19</date><risdate>1998</risdate><volume>222</volume><issue>2</issue><spage>269</spage><epage>278</epage><pages>269-278</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The chicken fibroblast cell line C32 has been transfected with the chicken homolog (Ch-IRF-1) of the mammalian transcription factor IRF-1. Stable transfectants were generated, constitutively overexpressing Ch-IRF-1 mRNA and protein. Cells overexpressing Ch-IRF-1 showed enhanced constitutive expression of MHC class I (B-F,
β-microglobulin) antigens. With increasing number of passages cells with normal B-F IV surface antigen expression accumulated. In the revertants, the amount of Ch-IRF-1 mRNA was reduced. Overexpression of Ch-IRF-1 had no effect on the constitutive expression and the induction by chicken interferon type-I and type-II (Ch-IFN) of guanylate-binding protein (GBP). Susceptibility to vesicular stomatitis virus, sindbis virus, Newcastle disease virus and vaccinia virus was not altered by overexpression of Ch-IRF-1. An antiviral state could be induced against all viruses tested by similar amounts of Ch-IFN type I in clone 20-18 expressing Ch-IRF-1 and cells transfected with empty vector.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9831662</pmid><doi>10.1016/S0378-1119(98)00504-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1998-11, Vol.222 (2), p.269-278 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70077701 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antiviral effect b-f antigen beta-microglubulin binding proteins Carrier Proteins - genetics Cell Line Chickens disease resistance DNA, Complementary - genetics DNA-Binding Proteins - genetics fibroblasts Gene Expression gene overexpression Guanosine Monophosphate - metabolism gyanylate-binding protein histocompatibility antigens Histocompatibility Antigens Class I - genetics IFN-signal transduction Interferon Regulatory Factor-1 interferons major histocompatibility complex Mammals messenger RNA Newcastle disease virus Newcastle disease virus - immunology Phosphoproteins - genetics Sindbis virus Sindbis Virus - immunology transcription factors Transcriptional activator transcriptional activators Transfection Vaccinia virus Vaccinia virus - immunology Vesicular stomatitis Indiana virus - immunology Vesicular stomatitis virus Vesiculovirus |
| title | Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line |
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