Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina
The electrogenic Na +–K + pump current (Ip) in carp bipolar cells was investigated under voltage-clamp conditions. The Ip was activated in a concentration-dependent manner by adding external K + (K + o), and was completely suppressed with 10 −4 M ouabain (EC 50=1.23 mM; Hill coefficient=1.36). The I...
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| Veröffentlicht in: | Neuropharmacology 1998-08, Vol.37 (8), p.1053-1061 |
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| container_title | Neuropharmacology |
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| creator | Zushi, Ikuko Shimura a, Masahiko Tamai, Makoto Kakazu, Yasuhiro Akaike, Norio |
| description | The electrogenic Na
+–K
+ pump current (Ip) in carp bipolar cells was investigated under voltage-clamp conditions. The Ip was activated in a concentration-dependent manner by adding external K
+ (K
+
o), and was completely suppressed with 10
−4 M ouabain (EC
50=1.23 mM; Hill coefficient=1.36). The Ip was suppressed in a concentration-dependent manner by ouabain (IC
50=1.90 mM; Hill coefficient=0.93). The Ip did not show a distinct voltage dependency either with or without Na
+
o. A large outward shift of the holding current was observed by completely removing Na
+
o. In the presence of Na
+
o, a steady Ip was observed even in the absence of internal Na
+ (Na
+
i). These results suggest that continuous Na
+ influxes exist across the membrane. When external and internal Na
+ was removed, a transient Ip was observed (half decay time (
t
1/2) was 5.0±0.6 s), thus indicating that the transient Ip was activated by the residual Na
+
i. In the absence of Na
+
o, the transient Ip was also observed with lower than 8 mM Na
+
i. The
t
1/2 depended on Na
+
i. However, a steady Ip was observed with 10 mM Na
+
i or more. The functional properties of the Ip are discussed. |
| doi_str_mv | 10.1016/S0028-3908(98)00084-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70074878</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0028390898000847</els_id><sourcerecordid>70074878</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-13a25be073d1f3fc68d1a18cb72e6659dd88dbd8c5cd4da679ebc6f49a7d63013</originalsourceid><addsrcrecordid>eNqFkM9u1DAQhy1EVZbCI1TyASFQFbDXiT0-VWjFP7WCA3C2JvaEGiVxsLNI9NR34A15ErLd1V45jUbzzcxPH2PnUrySQurXX4RYQ6WsgBcWXgohoK7MA7aSYFRlhK4fstURecQel_JjgWqQcMpOLSilVb1ibnODGf1MOd7iHNPIU8fnG-LUk59z-k5j9PwT8ou_d3-u-AWftsPE48jbOKUeM_fU94XHsjQzBd7lNHCPeeKZ5jjiE3bSYV_o6aGesW_v3n7dfKiuP7__uHlzXXkFdq6kwnXTkjAqyE51XkOQKMG3Zk1aNzYEgNAG8I0PdUBtLLVed7VFE7QSUp2x5_u7U04_t1RmN8Syy4YjpW1xRghTg4EFbPagz6mUTJ2bchww_3ZSuJ1Ydy_W7aw5C-5erDPL3vnhwbYdKBy3DiaX-bPDHIvHvss4-liO2LpWxjR2wS73GC0yfkXKrvhIo6cQ8yLchRT_E-Qf1AyV-A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70074878</pqid></control><display><type>article</type><title>Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Zushi, Ikuko ; Shimura a, Masahiko ; Tamai, Makoto ; Kakazu, Yasuhiro ; Akaike, Norio</creator><creatorcontrib>Zushi, Ikuko ; Shimura a, Masahiko ; Tamai, Makoto ; Kakazu, Yasuhiro ; Akaike, Norio</creatorcontrib><description>The electrogenic Na
+–K
+ pump current (Ip) in carp bipolar cells was investigated under voltage-clamp conditions. The Ip was activated in a concentration-dependent manner by adding external K
+ (K
+
o), and was completely suppressed with 10
−4 M ouabain (EC
50=1.23 mM; Hill coefficient=1.36). The Ip was suppressed in a concentration-dependent manner by ouabain (IC
50=1.90 mM; Hill coefficient=0.93). The Ip did not show a distinct voltage dependency either with or without Na
+
o. A large outward shift of the holding current was observed by completely removing Na
+
o. In the presence of Na
+
o, a steady Ip was observed even in the absence of internal Na
+ (Na
+
i). These results suggest that continuous Na
+ influxes exist across the membrane. When external and internal Na
+ was removed, a transient Ip was observed (half decay time (
t
1/2) was 5.0±0.6 s), thus indicating that the transient Ip was activated by the residual Na
+
i. In the absence of Na
+
o, the transient Ip was also observed with lower than 8 mM Na
+
i. The
t
1/2 depended on Na
+
i. However, a steady Ip was observed with 10 mM Na
+
i or more. The functional properties of the Ip are discussed.</description><identifier>ISSN: 0028-3908</identifier><identifier>EISSN: 1873-7064</identifier><identifier>DOI: 10.1016/S0028-3908(98)00084-7</identifier><identifier>PMID: 9833634</identifier><identifier>CODEN: NEPHBW</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Bipolar cell ; Carp ; Carps - anatomy & histology ; Carps - metabolism ; Cell Polarity ; Electrochemistry ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Isolated neuron and nerve. Neuroglia ; Ouabain - pharmacology ; Patch clamp ; Patch-Clamp Techniques ; Potassium - pharmacology ; Retina ; Retina - cytology ; Retina - metabolism ; Sodium-Potassium-Exchanging ATPase ; Sodium–potassium pump ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuropharmacology, 1998-08, Vol.37 (8), p.1053-1061</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-13a25be073d1f3fc68d1a18cb72e6659dd88dbd8c5cd4da679ebc6f49a7d63013</citedby><cites>FETCH-LOGICAL-c389t-13a25be073d1f3fc68d1a18cb72e6659dd88dbd8c5cd4da679ebc6f49a7d63013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0028-3908(98)00084-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2437759$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9833634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zushi, Ikuko</creatorcontrib><creatorcontrib>Shimura a, Masahiko</creatorcontrib><creatorcontrib>Tamai, Makoto</creatorcontrib><creatorcontrib>Kakazu, Yasuhiro</creatorcontrib><creatorcontrib>Akaike, Norio</creatorcontrib><title>Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina</title><title>Neuropharmacology</title><addtitle>Neuropharmacology</addtitle><description>The electrogenic Na
+–K
+ pump current (Ip) in carp bipolar cells was investigated under voltage-clamp conditions. The Ip was activated in a concentration-dependent manner by adding external K
+ (K
+
o), and was completely suppressed with 10
−4 M ouabain (EC
50=1.23 mM; Hill coefficient=1.36). The Ip was suppressed in a concentration-dependent manner by ouabain (IC
50=1.90 mM; Hill coefficient=0.93). The Ip did not show a distinct voltage dependency either with or without Na
+
o. A large outward shift of the holding current was observed by completely removing Na
+
o. In the presence of Na
+
o, a steady Ip was observed even in the absence of internal Na
+ (Na
+
i). These results suggest that continuous Na
+ influxes exist across the membrane. When external and internal Na
+ was removed, a transient Ip was observed (half decay time (
t
1/2) was 5.0±0.6 s), thus indicating that the transient Ip was activated by the residual Na
+
i. In the absence of Na
+
o, the transient Ip was also observed with lower than 8 mM Na
+
i. The
t
1/2 depended on Na
+
i. However, a steady Ip was observed with 10 mM Na
+
i or more. The functional properties of the Ip are discussed.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bipolar cell</subject><subject>Carp</subject><subject>Carps - anatomy & histology</subject><subject>Carps - metabolism</subject><subject>Cell Polarity</subject><subject>Electrochemistry</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Ouabain - pharmacology</subject><subject>Patch clamp</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium - pharmacology</subject><subject>Retina</subject><subject>Retina - cytology</subject><subject>Retina - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase</subject><subject>Sodium–potassium pump</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0028-3908</issn><issn>1873-7064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9u1DAQhy1EVZbCI1TyASFQFbDXiT0-VWjFP7WCA3C2JvaEGiVxsLNI9NR34A15ErLd1V45jUbzzcxPH2PnUrySQurXX4RYQ6WsgBcWXgohoK7MA7aSYFRlhK4fstURecQel_JjgWqQcMpOLSilVb1ibnODGf1MOd7iHNPIU8fnG-LUk59z-k5j9PwT8ou_d3-u-AWftsPE48jbOKUeM_fU94XHsjQzBd7lNHCPeeKZ5jjiE3bSYV_o6aGesW_v3n7dfKiuP7__uHlzXXkFdq6kwnXTkjAqyE51XkOQKMG3Zk1aNzYEgNAG8I0PdUBtLLVed7VFE7QSUp2x5_u7U04_t1RmN8Syy4YjpW1xRghTg4EFbPagz6mUTJ2bchww_3ZSuJ1Ydy_W7aw5C-5erDPL3vnhwbYdKBy3DiaX-bPDHIvHvss4-liO2LpWxjR2wS73GC0yfkXKrvhIo6cQ8yLchRT_E-Qf1AyV-A</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Zushi, Ikuko</creator><creator>Shimura a, Masahiko</creator><creator>Tamai, Makoto</creator><creator>Kakazu, Yasuhiro</creator><creator>Akaike, Norio</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina</title><author>Zushi, Ikuko ; Shimura a, Masahiko ; Tamai, Makoto ; Kakazu, Yasuhiro ; Akaike, Norio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-13a25be073d1f3fc68d1a18cb72e6659dd88dbd8c5cd4da679ebc6f49a7d63013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bipolar cell</topic><topic>Carp</topic><topic>Carps - anatomy & histology</topic><topic>Carps - metabolism</topic><topic>Cell Polarity</topic><topic>Electrochemistry</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Ouabain - pharmacology</topic><topic>Patch clamp</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium - pharmacology</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Retina - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase</topic><topic>Sodium–potassium pump</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zushi, Ikuko</creatorcontrib><creatorcontrib>Shimura a, Masahiko</creatorcontrib><creatorcontrib>Tamai, Makoto</creatorcontrib><creatorcontrib>Kakazu, Yasuhiro</creatorcontrib><creatorcontrib>Akaike, Norio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuropharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zushi, Ikuko</au><au>Shimura a, Masahiko</au><au>Tamai, Makoto</au><au>Kakazu, Yasuhiro</au><au>Akaike, Norio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina</atitle><jtitle>Neuropharmacology</jtitle><addtitle>Neuropharmacology</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>37</volume><issue>8</issue><spage>1053</spage><epage>1061</epage><pages>1053-1061</pages><issn>0028-3908</issn><eissn>1873-7064</eissn><coden>NEPHBW</coden><abstract>The electrogenic Na
+–K
+ pump current (Ip) in carp bipolar cells was investigated under voltage-clamp conditions. The Ip was activated in a concentration-dependent manner by adding external K
+ (K
+
o), and was completely suppressed with 10
−4 M ouabain (EC
50=1.23 mM; Hill coefficient=1.36). The Ip was suppressed in a concentration-dependent manner by ouabain (IC
50=1.90 mM; Hill coefficient=0.93). The Ip did not show a distinct voltage dependency either with or without Na
+
o. A large outward shift of the holding current was observed by completely removing Na
+
o. In the presence of Na
+
o, a steady Ip was observed even in the absence of internal Na
+ (Na
+
i). These results suggest that continuous Na
+ influxes exist across the membrane. When external and internal Na
+ was removed, a transient Ip was observed (half decay time (
t
1/2) was 5.0±0.6 s), thus indicating that the transient Ip was activated by the residual Na
+
i. In the absence of Na
+
o, the transient Ip was also observed with lower than 8 mM Na
+
i. The
t
1/2 depended on Na
+
i. However, a steady Ip was observed with 10 mM Na
+
i or more. The functional properties of the Ip are discussed.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9833634</pmid><doi>10.1016/S0028-3908(98)00084-7</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Neuropharmacology, 1998-08, Vol.37 (8), p.1053-1061 |
| issn | 0028-3908 1873-7064 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Bipolar cell Carp Carps - anatomy & histology Carps - metabolism Cell Polarity Electrochemistry Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Isolated neuron and nerve. Neuroglia Ouabain - pharmacology Patch clamp Patch-Clamp Techniques Potassium - pharmacology Retina Retina - cytology Retina - metabolism Sodium-Potassium-Exchanging ATPase Sodium–potassium pump Vertebrates: nervous system and sense organs |
| title | Characterization of the electrogenic Na +–K + pump in bipolar cells isolated from carp retina |
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