The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin
This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA 1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (...
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| Veröffentlicht in: | Biosensors & bioelectronics 1998-10, Vol.13 (7), p.779-785 |
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| container_title | Biosensors & bioelectronics |
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| creator | Chen, B. Bestetti, G. Day, R.M. Turner, A.P.F. |
| description | This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA
1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA
1c. The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. |
| doi_str_mv | 10.1016/S0956-5663(98)00042-6 |
| format | Article |
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1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA
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1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA
1c. The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin.</description><subject>affinity ligand</subject><subject>Animals</subject><subject>combinatorial peptide library</subject><subject>computer modelling simulation</subject><subject>Computer Simulation</subject><subject>diabetes</subject><subject>Diabetes Mellitus - blood</subject><subject>glycated proteins</subject><subject>glycosylated haemoglobin</subject><subject>Glycosylation</subject><subject>Hemoglobins - chemistry</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Ligands</subject><subject>one-bead-one-compound</subject><subject>Peptide Library</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtrHDEQhIWJcTZOfoJBpxAfxtZj9JhTCMZJDAYfbJ-FVmrtysyMJtKsYf69teslV58aur4u6CqELii5ooTK60fSCdkIKfmPTl8SQlrWyBO0olrxpmVcfEKr_8hn9KWUlwop2pEzdNZpprliKzQ9bQGXZZy3UGLBdvS4uAwwxnGDU8AWuzSs42jnlKPt8QTTHD3gPq6zzQsOKWMbQhzjvNTlphqUw3LTLy6VpbczeLy1MKRNn6rRV3QabF_g23Geo-fft083f5v7hz93N7_uG-CsnRsXyJqCU8I6LZzyjjPtQtcRUB4k09IrHqRTXAsmQQjtnfNCOU5YVVng5-j7u--U078dlNkMsTjoeztC2hWjahYt7eiHIJOaUCbVhyBVlGrR7h0vjuBuPYA3U45DzcocQ6_6z3cd6v-vEbIpLsLowMcMbjY-RUOJ2bdsDi2bfYX13BxaNpK_AfEkmqU</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Chen, B.</creator><creator>Bestetti, G.</creator><creator>Day, R.M.</creator><creator>Turner, A.P.F.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin</title><author>Chen, B. ; Bestetti, G. ; Day, R.M. ; Turner, A.P.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e324t-cf0b1ec75ac85c7dc328cf990e7de6286d73f6c738526e558dccd57c3026282f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>affinity ligand</topic><topic>Animals</topic><topic>combinatorial peptide library</topic><topic>computer modelling simulation</topic><topic>Computer Simulation</topic><topic>diabetes</topic><topic>Diabetes Mellitus - blood</topic><topic>glycated proteins</topic><topic>glycosylated haemoglobin</topic><topic>Glycosylation</topic><topic>Hemoglobins - chemistry</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Ligands</topic><topic>one-bead-one-compound</topic><topic>Peptide Library</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, B.</creatorcontrib><creatorcontrib>Bestetti, G.</creatorcontrib><creatorcontrib>Day, R.M.</creatorcontrib><creatorcontrib>Turner, A.P.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, B.</au><au>Bestetti, G.</au><au>Day, R.M.</au><au>Turner, A.P.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>13</volume><issue>7</issue><spage>779</spage><epage>785</epage><pages>779-785</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA
1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA
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| ispartof | Biosensors & bioelectronics, 1998-10, Vol.13 (7), p.779-785 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | affinity ligand Animals combinatorial peptide library computer modelling simulation Computer Simulation diabetes Diabetes Mellitus - blood glycated proteins glycosylated haemoglobin Glycosylation Hemoglobins - chemistry Hemoglobins - metabolism Humans Ligands one-bead-one-compound Peptide Library |
| title | The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin |
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