Effect of Chagas' disease on nitric oxide-containing neurons in severely affected and unaffected intestine
The pathophysiology of Chagas' disease is incompletely understood. Neuronal nitric oxide has been cited as a candidate neurotransmitter responsible for relaxation of the internal anal sphincter. Neuronal nicotinamide adenine dinucleotide phosphate diaphorase can be used as a marker for neuronal...
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| Veröffentlicht in: | Diseases of the colon & rectum 1998-11, Vol.41 (11), p.1411-1417 |
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| description | The pathophysiology of Chagas' disease is incompletely understood. Neuronal nitric oxide has been cited as a candidate neurotransmitter responsible for relaxation of the internal anal sphincter. Neuronal nicotinamide adenine dinucleotide phosphate diaphorase can be used as a marker for neuronal nitric oxide synthase. This study was designed to examine the alterations of the nitric oxide-containing neurons in the enteric nervous system of the colon of patients who underwent resections for advanced megacolon and to compare these specimens with small-bowel specimens from the same patients and with specimens from control subjects.
Specimens from resected rectum and extramucosal small-bowel biopsy specimens from 11 patients with Chagas megacolon but no apparent small-bowel clinical involvement were compared with the uninvolved colon and jejunum of 10 control patients with colon cancer. Tissues were fixed in Zamboni solution and evaluated by histochemistry for nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Reactivity was evaluated on a 0 to 4 scale in the longitudinal muscle, myenteric plexus, circular muscle, submucosal plexus, and mucosa.
Specimens from control patients showed well-stained myenteric and submucosal neurons and an abundant network of terminal nerve fibers in the muscle layers. Chagasic specimens had decreased staining in all layers of the gut. Overall there was a statistically significant decrease in nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Biopsy specimens from clinically uninvolved small bowel of patients with Chagas' disease also showed decreased reactivity, but to a lesser degree.
Nicotinamide adenine dinucleotide phosphate diaphorase activity is decreased in patients with advanced megacolon. The alterations are more relevant in the myenteric plexus and the circular muscle. Reactivity is also diminished in the clinically uninvolved small bowel, but to a lesser extent. |
| doi_str_mv | 10.1007/bf02237058 |
| format | Article |
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Specimens from resected rectum and extramucosal small-bowel biopsy specimens from 11 patients with Chagas megacolon but no apparent small-bowel clinical involvement were compared with the uninvolved colon and jejunum of 10 control patients with colon cancer. Tissues were fixed in Zamboni solution and evaluated by histochemistry for nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Reactivity was evaluated on a 0 to 4 scale in the longitudinal muscle, myenteric plexus, circular muscle, submucosal plexus, and mucosa.
Specimens from control patients showed well-stained myenteric and submucosal neurons and an abundant network of terminal nerve fibers in the muscle layers. Chagasic specimens had decreased staining in all layers of the gut. Overall there was a statistically significant decrease in nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Biopsy specimens from clinically uninvolved small bowel of patients with Chagas' disease also showed decreased reactivity, but to a lesser degree.
Nicotinamide adenine dinucleotide phosphate diaphorase activity is decreased in patients with advanced megacolon. The alterations are more relevant in the myenteric plexus and the circular muscle. Reactivity is also diminished in the clinically uninvolved small bowel, but to a lesser extent.</description><identifier>ISSN: 0012-3706</identifier><identifier>EISSN: 1530-0358</identifier><identifier>DOI: 10.1007/bf02237058</identifier><identifier>PMID: 9823808</identifier><identifier>CODEN: DICRAG</identifier><language>eng</language><publisher>Secaucus, NJ: Springer</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Chagas Disease - complications ; Chagas Disease - enzymology ; Chagas Disease - pathology ; Chagas Disease - physiopathology ; Female ; Human protozoal diseases ; Humans ; Infectious diseases ; Intestines - cytology ; Intestines - innervation ; Male ; Medical sciences ; Megacolon - enzymology ; Megacolon - etiology ; Megacolon - pathology ; Megacolon - physiopathology ; Middle Aged ; NADPH Dehydrogenase - metabolism ; Neurons - metabolism ; Neurons - pathology ; Nitric Oxide - metabolism ; Parasitic diseases ; Protozoal diseases ; Rectum - cytology ; Rectum - innervation ; Tropical medicine ; Trypanosomiasis</subject><ispartof>Diseases of the colon & rectum, 1998-11, Vol.41 (11), p.1411-1417</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-8bd719482cd6986996746c7f3ab40f0a5edb8e10837d2c0e18595431ef0ee6313</citedby><cites>FETCH-LOGICAL-c406t-8bd719482cd6986996746c7f3ab40f0a5edb8e10837d2c0e18595431ef0ee6313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1593702$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9823808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RIBEIRO, U. JR</creatorcontrib><creatorcontrib>SAFATLE-RIBEIRO, A. V</creatorcontrib><creatorcontrib>HABR-GAMA, A</creatorcontrib><creatorcontrib>GAMA-RODRIGUES, J. J</creatorcontrib><creatorcontrib>SOHN, J</creatorcontrib><creatorcontrib>REYNOLDS, J. C</creatorcontrib><title>Effect of Chagas' disease on nitric oxide-containing neurons in severely affected and unaffected intestine</title><title>Diseases of the colon & rectum</title><addtitle>Dis Colon Rectum</addtitle><description>The pathophysiology of Chagas' disease is incompletely understood. Neuronal nitric oxide has been cited as a candidate neurotransmitter responsible for relaxation of the internal anal sphincter. Neuronal nicotinamide adenine dinucleotide phosphate diaphorase can be used as a marker for neuronal nitric oxide synthase. This study was designed to examine the alterations of the nitric oxide-containing neurons in the enteric nervous system of the colon of patients who underwent resections for advanced megacolon and to compare these specimens with small-bowel specimens from the same patients and with specimens from control subjects.
Specimens from resected rectum and extramucosal small-bowel biopsy specimens from 11 patients with Chagas megacolon but no apparent small-bowel clinical involvement were compared with the uninvolved colon and jejunum of 10 control patients with colon cancer. Tissues were fixed in Zamboni solution and evaluated by histochemistry for nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Reactivity was evaluated on a 0 to 4 scale in the longitudinal muscle, myenteric plexus, circular muscle, submucosal plexus, and mucosa.
Specimens from control patients showed well-stained myenteric and submucosal neurons and an abundant network of terminal nerve fibers in the muscle layers. Chagasic specimens had decreased staining in all layers of the gut. Overall there was a statistically significant decrease in nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Biopsy specimens from clinically uninvolved small bowel of patients with Chagas' disease also showed decreased reactivity, but to a lesser degree.
Nicotinamide adenine dinucleotide phosphate diaphorase activity is decreased in patients with advanced megacolon. The alterations are more relevant in the myenteric plexus and the circular muscle. Reactivity is also diminished in the clinically uninvolved small bowel, but to a lesser extent.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Chagas Disease - complications</subject><subject>Chagas Disease - enzymology</subject><subject>Chagas Disease - pathology</subject><subject>Chagas Disease - physiopathology</subject><subject>Female</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Intestines - cytology</subject><subject>Intestines - innervation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Megacolon - enzymology</subject><subject>Megacolon - etiology</subject><subject>Megacolon - pathology</subject><subject>Megacolon - physiopathology</subject><subject>Middle Aged</subject><subject>NADPH Dehydrogenase - metabolism</subject><subject>Neurons - metabolism</subject><subject>Neurons - pathology</subject><subject>Nitric Oxide - metabolism</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Rectum - cytology</subject><subject>Rectum - innervation</subject><subject>Tropical medicine</subject><subject>Trypanosomiasis</subject><issn>0012-3706</issn><issn>1530-0358</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFLxDAQhYMouq5evAs5iIJQnTRtkh51cVVY8KLnkiYTjXTTNWnF_fdWXfU0PN7HN_AIOWJwwQDkZeMgz7mEUm2RCSs5ZMBLtU0mACzPxkLskf2UXscIOchdslupnCtQE_J64xyannaOzl70s05n1PqEOiHtAg2-j97Q7sNbzEwXeu2DD8804BC7kKgPNOE7RmzXVH-L0FIdLB3CX_Shx9T7gAdkx-k24eHmTsnT_OZxdpctHm7vZ1eLzBQg-kw1VrKqULmxolKiqoQshJGO66YAB7pE2yhkoLi0uQFkqqzKgjN0gCg441Ny-uNdxe5tGH_XS58Mtq0O2A2plgCiEkyO4PkPaGKXUkRXr6Jf6riuGdRfw9bX899hR_h4Yx2aJdo_dLPk2J9sep2Mbl3Uwfj0byyr0ZPzT_bZf7A</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>RIBEIRO, U. JR</creator><creator>SAFATLE-RIBEIRO, A. V</creator><creator>HABR-GAMA, A</creator><creator>GAMA-RODRIGUES, J. J</creator><creator>SOHN, J</creator><creator>REYNOLDS, J. C</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981101</creationdate><title>Effect of Chagas' disease on nitric oxide-containing neurons in severely affected and unaffected intestine</title><author>RIBEIRO, U. JR ; SAFATLE-RIBEIRO, A. V ; HABR-GAMA, A ; GAMA-RODRIGUES, J. J ; SOHN, J ; REYNOLDS, J. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-8bd719482cd6986996746c7f3ab40f0a5edb8e10837d2c0e18595431ef0ee6313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Chagas Disease - complications</topic><topic>Chagas Disease - enzymology</topic><topic>Chagas Disease - pathology</topic><topic>Chagas Disease - physiopathology</topic><topic>Female</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Intestines - cytology</topic><topic>Intestines - innervation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Megacolon - enzymology</topic><topic>Megacolon - etiology</topic><topic>Megacolon - pathology</topic><topic>Megacolon - physiopathology</topic><topic>Middle Aged</topic><topic>NADPH Dehydrogenase - metabolism</topic><topic>Neurons - metabolism</topic><topic>Neurons - pathology</topic><topic>Nitric Oxide - metabolism</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>Rectum - cytology</topic><topic>Rectum - innervation</topic><topic>Tropical medicine</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RIBEIRO, U. JR</creatorcontrib><creatorcontrib>SAFATLE-RIBEIRO, A. V</creatorcontrib><creatorcontrib>HABR-GAMA, A</creatorcontrib><creatorcontrib>GAMA-RODRIGUES, J. J</creatorcontrib><creatorcontrib>SOHN, J</creatorcontrib><creatorcontrib>REYNOLDS, J. C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diseases of the colon & rectum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RIBEIRO, U. JR</au><au>SAFATLE-RIBEIRO, A. V</au><au>HABR-GAMA, A</au><au>GAMA-RODRIGUES, J. J</au><au>SOHN, J</au><au>REYNOLDS, J. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Chagas' disease on nitric oxide-containing neurons in severely affected and unaffected intestine</atitle><jtitle>Diseases of the colon & rectum</jtitle><addtitle>Dis Colon Rectum</addtitle><date>1998-11-01</date><risdate>1998</risdate><volume>41</volume><issue>11</issue><spage>1411</spage><epage>1417</epage><pages>1411-1417</pages><issn>0012-3706</issn><eissn>1530-0358</eissn><coden>DICRAG</coden><abstract>The pathophysiology of Chagas' disease is incompletely understood. Neuronal nitric oxide has been cited as a candidate neurotransmitter responsible for relaxation of the internal anal sphincter. Neuronal nicotinamide adenine dinucleotide phosphate diaphorase can be used as a marker for neuronal nitric oxide synthase. This study was designed to examine the alterations of the nitric oxide-containing neurons in the enteric nervous system of the colon of patients who underwent resections for advanced megacolon and to compare these specimens with small-bowel specimens from the same patients and with specimens from control subjects.
Specimens from resected rectum and extramucosal small-bowel biopsy specimens from 11 patients with Chagas megacolon but no apparent small-bowel clinical involvement were compared with the uninvolved colon and jejunum of 10 control patients with colon cancer. Tissues were fixed in Zamboni solution and evaluated by histochemistry for nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Reactivity was evaluated on a 0 to 4 scale in the longitudinal muscle, myenteric plexus, circular muscle, submucosal plexus, and mucosa.
Specimens from control patients showed well-stained myenteric and submucosal neurons and an abundant network of terminal nerve fibers in the muscle layers. Chagasic specimens had decreased staining in all layers of the gut. Overall there was a statistically significant decrease in nicotinamide adenine dinucleotide phosphate diaphorase-containing neurons. Biopsy specimens from clinically uninvolved small bowel of patients with Chagas' disease also showed decreased reactivity, but to a lesser degree.
Nicotinamide adenine dinucleotide phosphate diaphorase activity is decreased in patients with advanced megacolon. The alterations are more relevant in the myenteric plexus and the circular muscle. Reactivity is also diminished in the clinically uninvolved small bowel, but to a lesser extent.</abstract><cop>Secaucus, NJ</cop><pub>Springer</pub><pmid>9823808</pmid><doi>10.1007/bf02237058</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Biological and medical sciences Chagas Disease - complications Chagas Disease - enzymology Chagas Disease - pathology Chagas Disease - physiopathology Female Human protozoal diseases Humans Infectious diseases Intestines - cytology Intestines - innervation Male Medical sciences Megacolon - enzymology Megacolon - etiology Megacolon - pathology Megacolon - physiopathology Middle Aged NADPH Dehydrogenase - metabolism Neurons - metabolism Neurons - pathology Nitric Oxide - metabolism Parasitic diseases Protozoal diseases Rectum - cytology Rectum - innervation Tropical medicine Trypanosomiasis |
| title | Effect of Chagas' disease on nitric oxide-containing neurons in severely affected and unaffected intestine |
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