A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows

This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dair...

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Veröffentlicht in:Journal of Reproduction and Development 2007, Vol.53(6), pp.1313-1318
Hauptverfasser: Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.)), Choe, C.Y, Cho, S.R, Choi, S.H, Kim, H.J, Hur, T.Y, Jung, Y.G, Kang, H.G, Kim, I.H
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container_issue 6
container_start_page 1313
container_title Journal of Reproduction and Development
container_volume 53
creator Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))
Choe, C.Y
Cho, S.R
Choi, S.H
Kim, H.J
Hur, T.Y
Jung, Y.G
Kang, H.G
Kim, I.H
description This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.
doi_str_mv 10.1262/jrd.19066
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Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). 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Reprod. Dev.</addtitle><description>This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.</description><subject>Animals</subject><subject>ARTIFICIAL INSEMINATION</subject><subject>Breeding</subject><subject>Cattle</subject><subject>CHOIX DE LA DATE</subject><subject>Controlled internal drug release (CIDR)</subject><subject>CONTROLLED RELEASE</subject><subject>DAIRY COWS</subject><subject>Dairying</subject><subject>Drug Implants</subject><subject>DRUGS</subject><subject>ELECCION DE LA EPOCA</subject><subject>EMBRYO TRANSFER</subject><subject>Embryo Transfer - methods</subject><subject>Embryo Transfer - veterinary</subject><subject>ESTERILIDAD FEMENINA</subject><subject>Estrus Synchronization</subject><subject>Female</subject><subject>FEMALE INFERTILITY</subject><subject>INFERTILITE FEMELLE</subject><subject>INSEMINACION ARTIFICIAL</subject><subject>INSEMINATION ARTIFICIELLE</subject><subject>Insemination, Artificial - methods</subject><subject>Insemination, Artificial - veterinary</subject><subject>LACTACION</subject><subject>Lactating repeat breeder dairy cow</subject><subject>LACTATION</subject><subject>LIBERACION CONTROLADA</subject><subject>LIBERATION CONTROLEE</subject><subject>MEDICAMENT</subject><subject>MEDICAMENTOS</subject><subject>PERFORMANCE DE REPRODUCTION</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Progesterone - blood</subject><subject>REPRODUCTIVE PERFORMANCE</subject><subject>REPRODUCTIVIDAD</subject><subject>Timed artificial insemination (TAI)</subject><subject>Timed embryo transfer (TET)</subject><subject>TIMING</subject><subject>TRANSFERENCIA DE EMBRIONES</subject><subject>TRANSFERT EMBRYONNAIRE</subject><subject>VACAS LECHERAS</subject><subject>VACHE LAITIERE</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1rGzEQhkVpaNy0h_6AFp0CPWw6Wu1qtacS3K-UQEtIz2JWGjlr1itXkgn-91Vsk15mQPPwoHmHsXcCrkSt6k_r6K5ED0q9YAshG101DcBLtoBeqEproc_Z65TWALJuVfOKnYtO153WsGDhmi9vvtxVAyZyPI-bUmkzxH3gOeKcPEW-jSEHGyY-zjZSARPPD1SeaTXjbPc8YiYePJ_QZszjvOKRtoSZD5HIFYPDMe65DY_pDTvzOCV6e-oX7M-3r_fLH9Xtr-83y-vbyrZK58r5oXNaa5QOhPV1o7p-gM6T946Es2VBSbrxfds40Mqj7ctKqqs9kJca5AW7PHrL5__uKGWzGZOlacKZwi6ZDkDpVokCfjyCNoaUInmzjeMG494IME_pmpKuOaRb2A8n6W4oQf0nT3EW4PMRWKeMK3oGMObRTnRQtdKop3JQPk_sA0ZDczG8Pxo8BoOrOCbz83cNoMvxZNvLfwjulkc</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</creator><creator>Choe, C.Y</creator><creator>Cho, S.R</creator><creator>Choi, S.H</creator><creator>Kim, H.J</creator><creator>Hur, T.Y</creator><creator>Jung, Y.G</creator><creator>Kang, H.G</creator><creator>Kim, I.H</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows</title><author>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.)) ; Choe, C.Y ; Cho, S.R ; Choi, S.H ; Kim, H.J ; Hur, T.Y ; Jung, Y.G ; Kang, H.G ; Kim, I.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-dfb7d888a3d01cf24679b07feffde1dc4403e84f954d086fac9782672f0ef3803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>ARTIFICIAL INSEMINATION</topic><topic>Breeding</topic><topic>Cattle</topic><topic>CHOIX DE LA DATE</topic><topic>Controlled internal drug release (CIDR)</topic><topic>CONTROLLED RELEASE</topic><topic>DAIRY COWS</topic><topic>Dairying</topic><topic>Drug Implants</topic><topic>DRUGS</topic><topic>ELECCION DE LA EPOCA</topic><topic>EMBRYO TRANSFER</topic><topic>Embryo Transfer - methods</topic><topic>Embryo Transfer - veterinary</topic><topic>ESTERILIDAD FEMENINA</topic><topic>Estrus Synchronization</topic><topic>Female</topic><topic>FEMALE INFERTILITY</topic><topic>INFERTILITE FEMELLE</topic><topic>INSEMINACION ARTIFICIAL</topic><topic>INSEMINATION ARTIFICIELLE</topic><topic>Insemination, Artificial - methods</topic><topic>Insemination, Artificial - veterinary</topic><topic>LACTACION</topic><topic>Lactating repeat breeder dairy cow</topic><topic>LACTATION</topic><topic>LIBERACION CONTROLADA</topic><topic>LIBERATION CONTROLEE</topic><topic>MEDICAMENT</topic><topic>MEDICAMENTOS</topic><topic>PERFORMANCE DE REPRODUCTION</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Progesterone - blood</topic><topic>REPRODUCTIVE PERFORMANCE</topic><topic>REPRODUCTIVIDAD</topic><topic>Timed artificial insemination (TAI)</topic><topic>Timed embryo transfer (TET)</topic><topic>TIMING</topic><topic>TRANSFERENCIA DE EMBRIONES</topic><topic>TRANSFERT EMBRYONNAIRE</topic><topic>VACAS LECHERAS</topic><topic>VACHE LAITIERE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</creatorcontrib><creatorcontrib>Choe, C.Y</creatorcontrib><creatorcontrib>Cho, S.R</creatorcontrib><creatorcontrib>Choi, S.H</creatorcontrib><creatorcontrib>Kim, H.J</creatorcontrib><creatorcontrib>Hur, T.Y</creatorcontrib><creatorcontrib>Jung, Y.G</creatorcontrib><creatorcontrib>Kang, H.G</creatorcontrib><creatorcontrib>Kim, I.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</au><au>Choe, C.Y</au><au>Cho, S.R</au><au>Choi, S.H</au><au>Kim, H.J</au><au>Hur, T.Y</au><au>Jung, Y.G</au><au>Kang, H.G</au><au>Kim, I.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>53</volume><issue>6</issue><spage>1313</spage><epage>1318</epage><pages>1313-1318</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>17827880</pmid><doi>10.1262/jrd.19066</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Animals
ARTIFICIAL INSEMINATION
Breeding
Cattle
CHOIX DE LA DATE
Controlled internal drug release (CIDR)
CONTROLLED RELEASE
DAIRY COWS
Dairying
Drug Implants
DRUGS
ELECCION DE LA EPOCA
EMBRYO TRANSFER
Embryo Transfer - methods
Embryo Transfer - veterinary
ESTERILIDAD FEMENINA
Estrus Synchronization
Female
FEMALE INFERTILITY
INFERTILITE FEMELLE
INSEMINACION ARTIFICIAL
INSEMINATION ARTIFICIELLE
Insemination, Artificial - methods
Insemination, Artificial - veterinary
LACTACION
Lactating repeat breeder dairy cow
LACTATION
LIBERACION CONTROLADA
LIBERATION CONTROLEE
MEDICAMENT
MEDICAMENTOS
PERFORMANCE DE REPRODUCTION
Pregnancy
Pregnancy Rate
Progesterone - blood
REPRODUCTIVE PERFORMANCE
REPRODUCTIVIDAD
Timed artificial insemination (TAI)
Timed embryo transfer (TET)
TIMING
TRANSFERENCIA DE EMBRIONES
TRANSFERT EMBRYONNAIRE
VACAS LECHERAS
VACHE LAITIERE
title A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows
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