A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows
This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dair...
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Veröffentlicht in: | Journal of Reproduction and Development 2007, Vol.53(6), pp.1313-1318 |
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creator | Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.)) Choe, C.Y Cho, S.R Choi, S.H Kim, H.J Hur, T.Y Jung, Y.G Kang, H.G Kim, I.H |
description | This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows. |
doi_str_mv | 10.1262/jrd.19066 |
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Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.19066</identifier><identifier>PMID: 17827880</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>Animals ; ARTIFICIAL INSEMINATION ; Breeding ; Cattle ; CHOIX DE LA DATE ; Controlled internal drug release (CIDR) ; CONTROLLED RELEASE ; DAIRY COWS ; Dairying ; Drug Implants ; DRUGS ; ELECCION DE LA EPOCA ; EMBRYO TRANSFER ; Embryo Transfer - methods ; Embryo Transfer - veterinary ; ESTERILIDAD FEMENINA ; Estrus Synchronization ; Female ; FEMALE INFERTILITY ; INFERTILITE FEMELLE ; INSEMINACION ARTIFICIAL ; INSEMINATION ARTIFICIELLE ; Insemination, Artificial - methods ; Insemination, Artificial - veterinary ; LACTACION ; Lactating repeat breeder dairy cow ; LACTATION ; LIBERACION CONTROLADA ; LIBERATION CONTROLEE ; MEDICAMENT ; MEDICAMENTOS ; PERFORMANCE DE REPRODUCTION ; Pregnancy ; Pregnancy Rate ; Progesterone - blood ; REPRODUCTIVE PERFORMANCE ; REPRODUCTIVIDAD ; Timed artificial insemination (TAI) ; Timed embryo transfer (TET) ; TIMING ; TRANSFERENCIA DE EMBRIONES ; TRANSFERT EMBRYONNAIRE ; VACAS LECHERAS ; VACHE LAITIERE</subject><ispartof>Journal of Reproduction and Development, 2007, Vol.53(6), pp.1313-1318</ispartof><rights>2007 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-dfb7d888a3d01cf24679b07feffde1dc4403e84f954d086fac9782672f0ef3803</citedby><cites>FETCH-LOGICAL-c568t-dfb7d888a3d01cf24679b07feffde1dc4403e84f954d086fac9782672f0ef3803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17827880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</creatorcontrib><creatorcontrib>Choe, C.Y</creatorcontrib><creatorcontrib>Cho, S.R</creatorcontrib><creatorcontrib>Choi, S.H</creatorcontrib><creatorcontrib>Kim, H.J</creatorcontrib><creatorcontrib>Hur, T.Y</creatorcontrib><creatorcontrib>Jung, Y.G</creatorcontrib><creatorcontrib>Kang, H.G</creatorcontrib><creatorcontrib>Kim, I.H</creatorcontrib><title>A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.</description><subject>Animals</subject><subject>ARTIFICIAL INSEMINATION</subject><subject>Breeding</subject><subject>Cattle</subject><subject>CHOIX DE LA DATE</subject><subject>Controlled internal drug release (CIDR)</subject><subject>CONTROLLED RELEASE</subject><subject>DAIRY COWS</subject><subject>Dairying</subject><subject>Drug Implants</subject><subject>DRUGS</subject><subject>ELECCION DE LA EPOCA</subject><subject>EMBRYO TRANSFER</subject><subject>Embryo Transfer - methods</subject><subject>Embryo Transfer - veterinary</subject><subject>ESTERILIDAD FEMENINA</subject><subject>Estrus Synchronization</subject><subject>Female</subject><subject>FEMALE INFERTILITY</subject><subject>INFERTILITE FEMELLE</subject><subject>INSEMINACION ARTIFICIAL</subject><subject>INSEMINATION ARTIFICIELLE</subject><subject>Insemination, Artificial - methods</subject><subject>Insemination, Artificial - veterinary</subject><subject>LACTACION</subject><subject>Lactating repeat breeder dairy cow</subject><subject>LACTATION</subject><subject>LIBERACION CONTROLADA</subject><subject>LIBERATION CONTROLEE</subject><subject>MEDICAMENT</subject><subject>MEDICAMENTOS</subject><subject>PERFORMANCE DE REPRODUCTION</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Progesterone - blood</subject><subject>REPRODUCTIVE PERFORMANCE</subject><subject>REPRODUCTIVIDAD</subject><subject>Timed artificial insemination (TAI)</subject><subject>Timed embryo transfer (TET)</subject><subject>TIMING</subject><subject>TRANSFERENCIA DE EMBRIONES</subject><subject>TRANSFERT EMBRYONNAIRE</subject><subject>VACAS LECHERAS</subject><subject>VACHE LAITIERE</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1rGzEQhkVpaNy0h_6AFp0CPWw6Wu1qtacS3K-UQEtIz2JWGjlr1itXkgn-91Vsk15mQPPwoHmHsXcCrkSt6k_r6K5ED0q9YAshG101DcBLtoBeqEproc_Z65TWALJuVfOKnYtO153WsGDhmi9vvtxVAyZyPI-bUmkzxH3gOeKcPEW-jSEHGyY-zjZSARPPD1SeaTXjbPc8YiYePJ_QZszjvOKRtoSZD5HIFYPDMe65DY_pDTvzOCV6e-oX7M-3r_fLH9Xtr-83y-vbyrZK58r5oXNaa5QOhPV1o7p-gM6T946Es2VBSbrxfds40Mqj7ctKqqs9kJca5AW7PHrL5__uKGWzGZOlacKZwi6ZDkDpVokCfjyCNoaUInmzjeMG494IME_pmpKuOaRb2A8n6W4oQf0nT3EW4PMRWKeMK3oGMObRTnRQtdKop3JQPk_sA0ZDczG8Pxo8BoOrOCbz83cNoMvxZNvLfwjulkc</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</creator><creator>Choe, C.Y</creator><creator>Cho, S.R</creator><creator>Choi, S.H</creator><creator>Kim, H.J</creator><creator>Hur, T.Y</creator><creator>Jung, Y.G</creator><creator>Kang, H.G</creator><creator>Kim, I.H</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows</title><author>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.)) ; Choe, C.Y ; Cho, S.R ; Choi, S.H ; Kim, H.J ; Hur, T.Y ; Jung, Y.G ; Kang, H.G ; Kim, I.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-dfb7d888a3d01cf24679b07feffde1dc4403e84f954d086fac9782672f0ef3803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>ARTIFICIAL INSEMINATION</topic><topic>Breeding</topic><topic>Cattle</topic><topic>CHOIX DE LA DATE</topic><topic>Controlled internal drug release (CIDR)</topic><topic>CONTROLLED RELEASE</topic><topic>DAIRY COWS</topic><topic>Dairying</topic><topic>Drug Implants</topic><topic>DRUGS</topic><topic>ELECCION DE LA EPOCA</topic><topic>EMBRYO TRANSFER</topic><topic>Embryo Transfer - methods</topic><topic>Embryo Transfer - veterinary</topic><topic>ESTERILIDAD FEMENINA</topic><topic>Estrus Synchronization</topic><topic>Female</topic><topic>FEMALE INFERTILITY</topic><topic>INFERTILITE FEMELLE</topic><topic>INSEMINACION ARTIFICIAL</topic><topic>INSEMINATION ARTIFICIELLE</topic><topic>Insemination, Artificial - methods</topic><topic>Insemination, Artificial - veterinary</topic><topic>LACTACION</topic><topic>Lactating repeat breeder dairy cow</topic><topic>LACTATION</topic><topic>LIBERACION CONTROLADA</topic><topic>LIBERATION CONTROLEE</topic><topic>MEDICAMENT</topic><topic>MEDICAMENTOS</topic><topic>PERFORMANCE DE REPRODUCTION</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Progesterone - blood</topic><topic>REPRODUCTIVE PERFORMANCE</topic><topic>REPRODUCTIVIDAD</topic><topic>Timed artificial insemination (TAI)</topic><topic>Timed embryo transfer (TET)</topic><topic>TIMING</topic><topic>TRANSFERENCIA DE EMBRIONES</topic><topic>TRANSFERT EMBRYONNAIRE</topic><topic>VACAS LECHERAS</topic><topic>VACHE LAITIERE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</creatorcontrib><creatorcontrib>Choe, C.Y</creatorcontrib><creatorcontrib>Cho, S.R</creatorcontrib><creatorcontrib>Choi, S.H</creatorcontrib><creatorcontrib>Kim, H.J</creatorcontrib><creatorcontrib>Hur, T.Y</creatorcontrib><creatorcontrib>Jung, Y.G</creatorcontrib><creatorcontrib>Kang, H.G</creatorcontrib><creatorcontrib>Kim, I.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, D.S.(National Inst. of Animal Science, Namwon (Korea R.))</au><au>Choe, C.Y</au><au>Cho, S.R</au><au>Choi, S.H</au><au>Kim, H.J</au><au>Hur, T.Y</au><au>Jung, Y.G</au><au>Kang, H.G</au><au>Kim, I.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>53</volume><issue>6</issue><spage>1313</spage><epage>1318</epage><pages>1313-1318</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGFsub(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGFsub(2alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGFsub(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P0.01) and subsequently increased by Day 16 (P0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>17827880</pmid><doi>10.1262/jrd.19066</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | J-STAGE Free; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
subjects | Animals ARTIFICIAL INSEMINATION Breeding Cattle CHOIX DE LA DATE Controlled internal drug release (CIDR) CONTROLLED RELEASE DAIRY COWS Dairying Drug Implants DRUGS ELECCION DE LA EPOCA EMBRYO TRANSFER Embryo Transfer - methods Embryo Transfer - veterinary ESTERILIDAD FEMENINA Estrus Synchronization Female FEMALE INFERTILITY INFERTILITE FEMELLE INSEMINACION ARTIFICIAL INSEMINATION ARTIFICIELLE Insemination, Artificial - methods Insemination, Artificial - veterinary LACTACION Lactating repeat breeder dairy cow LACTATION LIBERACION CONTROLADA LIBERATION CONTROLEE MEDICAMENT MEDICAMENTOS PERFORMANCE DE REPRODUCTION Pregnancy Pregnancy Rate Progesterone - blood REPRODUCTIVE PERFORMANCE REPRODUCTIVIDAD Timed artificial insemination (TAI) Timed embryo transfer (TET) TIMING TRANSFERENCIA DE EMBRIONES TRANSFERT EMBRYONNAIRE VACAS LECHERAS VACHE LAITIERE |
title | A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows |
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