Quantitative biochemical analysis of samples from squamous cell carcinoma
Samples of tumor, tumor stroma, and mixed tumor and stroma were microdissected from three squamous cell carcinomas, as well as from adjacent nontumor tissue and assayed for malate dehydrogenase or lactate dehydrogenase activity as indicators of the relative contribution of aerobic and anaerobic proc...
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| Veröffentlicht in: | Otolaryngology-head and neck surgery 1998-11, Vol.119 (5), p.455-459 |
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| container_title | Otolaryngology-head and neck surgery |
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| creator | ROSS, C.DAVID MEDINA, JESUS E. |
| description | Samples of tumor, tumor stroma, and mixed tumor and stroma were microdissected from three squamous cell carcinomas, as well as from adjacent nontumor tissue and assayed for malate dehydrogenase or lactate dehydrogenase activity as indicators of the relative contribution of aerobic and anaerobic processes, respectively, to energy metabolism. Malate and lactate dehydrogenase activities were greater in tumor tissue than in tumor stroma in all three tumors. However, the magnitudes of malate and lactate dehydrogenase activities were different among the tumors, suggesting that tumors vary in the degree to which aerobic and anaerobic reactions contribute to energy production. These results indicate that a quantitative assessment of enzymes involved in energy production may be a useful probe in the understanding of tumor metabolism. |
| doi_str_mv | 10.1016/S0194-5998(98)70101-9 |
| format | Article |
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Malate and lactate dehydrogenase activities were greater in tumor tissue than in tumor stroma in all three tumors. However, the magnitudes of malate and lactate dehydrogenase activities were different among the tumors, suggesting that tumors vary in the degree to which aerobic and anaerobic reactions contribute to energy production. 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Malate and lactate dehydrogenase activities were greater in tumor tissue than in tumor stroma in all three tumors. However, the magnitudes of malate and lactate dehydrogenase activities were different among the tumors, suggesting that tumors vary in the degree to which aerobic and anaerobic reactions contribute to energy production. These results indicate that a quantitative assessment of enzymes involved in energy production may be a useful probe in the understanding of tumor metabolism.</description><subject>Carcinoma, Squamous Cell - enzymology</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Energy Metabolism</subject><subject>Humans</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Malate Dehydrogenase - metabolism</subject><issn>0194-5998</issn><issn>1097-6817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1KxDAUhYMoOo4-gtCV6KKadJqkWYio-AeDIroPt-mtRtpmTFpl3t6MHdwqBC7knnNy8hFywOgJo0ycPlOm8pQrVRyp4ljSeJmqDTJhVMlUFExuksmvZIfshvBOKRVCym2yrQoqqSgm5P5pgK63PfT2E5PSOvOGrTXQJNBBsww2JK5OArSLBkNSe9cm4WOA1g0hMdg0iQFvbOda2CNbNTQB99dzSl5url-u7tL54-391cU8NTmXPM2gFEbmalZkUlHDQMV6rKpMLYuZBAo11HGW3FQl4qzKMpGxitelLAtR5rMpORxjF959DBh63dqwagIdxlJaxj_yPBdRyEeh8S4Ej7VeeNuCX2pG9Yqg_iGoV3h0PD8EtYq-g_UDQ9li9etaI4v7s3H_ZRtc_i9UP949XN5kUnAe_dnoD_CK-t0NPoIOf5Y6H00YyX5a9DoYi53Byno0va6c_SPhG90molY</recordid><startdate>199811</startdate><enddate>199811</enddate><creator>ROSS, C.DAVID</creator><creator>MEDINA, JESUS E.</creator><general>Mosby, Inc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>199811</creationdate><title>Quantitative biochemical analysis of samples from squamous cell carcinoma</title><author>ROSS, C.DAVID ; MEDINA, JESUS E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4575-2ab6c749382790c1a99981ddcf7837a0afaf37ab5cdbee3d22621d5fb7b86b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Carcinoma, Squamous Cell - enzymology</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Energy Metabolism</topic><topic>Humans</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Malate Dehydrogenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROSS, C.DAVID</creatorcontrib><creatorcontrib>MEDINA, JESUS E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Otolaryngology-head and neck surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROSS, C.DAVID</au><au>MEDINA, JESUS E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative biochemical analysis of samples from squamous cell carcinoma</atitle><jtitle>Otolaryngology-head and neck surgery</jtitle><addtitle>Otolaryngol Head Neck Surg</addtitle><date>1998-11</date><risdate>1998</risdate><volume>119</volume><issue>5</issue><spage>455</spage><epage>459</epage><pages>455-459</pages><issn>0194-5998</issn><eissn>1097-6817</eissn><abstract>Samples of tumor, tumor stroma, and mixed tumor and stroma were microdissected from three squamous cell carcinomas, as well as from adjacent nontumor tissue and assayed for malate dehydrogenase or lactate dehydrogenase activity as indicators of the relative contribution of aerobic and anaerobic processes, respectively, to energy metabolism. Malate and lactate dehydrogenase activities were greater in tumor tissue than in tumor stroma in all three tumors. However, the magnitudes of malate and lactate dehydrogenase activities were different among the tumors, suggesting that tumors vary in the degree to which aerobic and anaerobic reactions contribute to energy production. These results indicate that a quantitative assessment of enzymes involved in energy production may be a useful probe in the understanding of tumor metabolism.</abstract><cop>Los Angeles, CA</cop><pub>Mosby, Inc</pub><pmid>9807068</pmid><doi>10.1016/S0194-5998(98)70101-9</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0194-5998 |
| ispartof | Otolaryngology-head and neck surgery, 1998-11, Vol.119 (5), p.455-459 |
| issn | 0194-5998 1097-6817 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Carcinoma, Squamous Cell - enzymology Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Energy Metabolism Humans L-Lactate Dehydrogenase - metabolism Malate Dehydrogenase - metabolism |
| title | Quantitative biochemical analysis of samples from squamous cell carcinoma |
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