Quantitative imaging of TATA‐binding protein in living yeast cells

We describe the quantitative monitoring of TATA‐binding protein (TBP) localization and expression in living Saccharomyces cerevisiae cells. We replaced the endogenous TBP with a green fluorescent protein (GFP) · TBP fusion, which was imaged quantitatively by laser scanning confocal microscopy (LSCM)...

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Veröffentlicht in:	Yeast (Chichester, England) England), 1998-06, Vol.14 (9), p.813-825
Hauptverfasser:	Patterson, George H., Schroeder, Stephanie C., Bai, Yu, Weil, P. Anthony, Piston, David W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Nucleus - chemistry DNA-Binding Proteins - analysis DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Regulation, Fungal Green Fluorescent Proteins Immunoblotting Indicators and Reagents Luminescent Proteins - metabolism Microscopy, Confocal Mitosis Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Subcellular Fractions - chemistry TATA Box TATA-Box Binding Protein Time Factors Transcription Factors - analysis Transcription Factors - genetics Transcription Factors - metabolism
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