Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma
SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2...
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| Veröffentlicht in: | Molecules and cells 2007-12, Vol.24 (3), p.323-328 |
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| creator | Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan) Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan) Sato, Youichi (The University of Tokushima, Tokushima, Japan) Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan) Chen, Gang (The University of Tokushima, Tokushima, Japan) Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan) Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan) Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan) Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp |
| description | SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle. |
| doi_str_mv | 10.1016/s1016-8478(23)07346-6 |
| format | Article |
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However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. 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However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.</description><subject>Carcinoma, Embryonal - pathology</subject><subject>CELL CYCLE</subject><subject>Cell Line, Tumor</subject><subject>cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>CICLO CELULAR</subject><subject>CYCLE CELLULAIRE</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>Flow Cytometry</subject><subject>Gene Expression</subject><subject>High Mobility Group Proteins - biosynthesis</subject><subject>Humans</subject><subject>Male</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>SOX</subject><subject>SOX Transcription Factors</subject><subject>SOX15</subject><subject>Testicular Neoplasms - pathology</subject><issn>1016-8478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFFPwjAQx_ugEUQ_AqZPRh8mbbd13aMZqEQiRjDxrWm7Tmu2FdpB5Ns7YNGXu-Tud3f_-wMwxOgOI0xHfh8DFiXshoS3KAkjGtAT0P8r98C5998I4YQSdgZ6mGFGWET7YD3faqd_Vk57b2wNbQEX8w8cw2n9ZaRpPHx1tjSFdqLp-i9LMhpjmOmy9HCsndnqHBbOVlDApfaNUZtSODippNvZWpQHEmbCKVPbSlyA00KUXl92eQDeHybL7CmYzR-n2f0sUFFEmoBIyWROmNRIaYIwKbDEStIE5zQkqaYqTWWM4_0bKIyFSHWR5iqnhBKUJ1E4ANfHvStn15tWF6-MV60UUWu78TxBKE5JzFowPoLKWe-dLvjKmUq4HceI7y3ki0PcG8lJyA_-ctrOXXUHNrLS-f9UZ24LDI9AISwXn854_vxGEGIIkShMwl-7JIDN</recordid><startdate>20071231</startdate><enddate>20071231</enddate><creator>Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan)</creator><creator>Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan)</creator><creator>Sato, Youichi (The University of Tokushima, Tokushima, Japan)</creator><creator>Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan)</creator><creator>Chen, Gang (The University of Tokushima, Tokushima, Japan)</creator><creator>Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan)</creator><creator>Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan)</creator><creator>Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan)</creator><creator>Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071231</creationdate><title>Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma</title><author>Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan) ; Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan) ; Sato, Youichi (The University of Tokushima, Tokushima, Japan) ; Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan) ; Chen, Gang (The University of Tokushima, Tokushima, Japan) ; Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan) ; Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan) ; Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan) ; Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-2bb8bd28be0ce2012f1b1cb671d6329e6c99b5152846035aa9ef9dcd62620d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Carcinoma, Embryonal - pathology</topic><topic>CELL CYCLE</topic><topic>Cell Line, Tumor</topic><topic>cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>CICLO CELULAR</topic><topic>CYCLE CELLULAIRE</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>Flow Cytometry</topic><topic>Gene Expression</topic><topic>High Mobility Group Proteins - biosynthesis</topic><topic>Humans</topic><topic>Male</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>SOX</topic><topic>SOX Transcription Factors</topic><topic>SOX15</topic><topic>Testicular Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Sato, Youichi (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Chen, Gang (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan)</creatorcontrib><creatorcontrib>Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan)</creatorcontrib><creatorcontrib>Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecules and cells</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan)</au><au>Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan)</au><au>Sato, Youichi (The University of Tokushima, Tokushima, Japan)</au><au>Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan)</au><au>Chen, Gang (The University of Tokushima, Tokushima, Japan)</au><au>Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan)</au><au>Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan)</au><au>Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan)</au><au>Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma</atitle><jtitle>Molecules and cells</jtitle><addtitle>Mol Cells</addtitle><date>2007-12-31</date><risdate>2007</risdate><volume>24</volume><issue>3</issue><spage>323</spage><epage>328</epage><pages>323-328</pages><issn>1016-8478</issn><abstract>SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.</abstract><cop>United States</cop><pmid>18182846</pmid><doi>10.1016/s1016-8478(23)07346-6</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Carcinoma, Embryonal - pathology CELL CYCLE Cell Line, Tumor cell proliferation Cell Proliferation - drug effects CICLO CELULAR CYCLE CELLULAIRE DNA-Binding Proteins - biosynthesis Flow Cytometry Gene Expression High Mobility Group Proteins - biosynthesis Humans Male Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism SOX SOX Transcription Factors SOX15 Testicular Neoplasms - pathology |
| title | Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma |
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