In vivo imaging of spontaneous ultraweak photon emission from a rat’s brain correlated with cerebral energy metabolism and oxidative stress

Living cells spontaneously emit ultraweak light during the process of metabolic reactions associated with the physiological state. The first demonstration of two-dimensional in vivo imaging of ultraweak photon emission from a rat’s brain, using a highly sensitive photon counting apparatus, is report...

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Veröffentlicht in:Neuroscience research 1999-07, Vol.34 (2), p.103-113
Hauptverfasser: Kobayashi, Masaki, Takeda, Motohiro, Sato, Tomoo, Yamazaki, Yoshihiko, Kaneko, Kenya, Ito, Ken-Ichi, Kato, Hiroshi, Inaba, Humio
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container_end_page 113
container_issue 2
container_start_page 103
container_title Neuroscience research
container_volume 34
creator Kobayashi, Masaki
Takeda, Motohiro
Sato, Tomoo
Yamazaki, Yoshihiko
Kaneko, Kenya
Ito, Ken-Ichi
Kato, Hiroshi
Inaba, Humio
description Living cells spontaneously emit ultraweak light during the process of metabolic reactions associated with the physiological state. The first demonstration of two-dimensional in vivo imaging of ultraweak photon emission from a rat’s brain, using a highly sensitive photon counting apparatus, is reported in this paper. It was found that the emission intensity correlates with the electroencephalographic activity that was measured on the cortical surface and this intensity is associated with the cerebral blood flow and hyperoxia. To clarify the mechanism of photon emission, intensity changes from whole brain slices were examined under various conditions. The removal of glucose from the incubation medium suppressed the photon emission, and adding 50 mM potassium ions led to temporal enhancement of emission and subsequent depression. Rotenone (20 μM), an inhibitor of the mitochondrial electron transport chain, increased photon emission, indicating electron leakage from the respiratory chain. These results suggest that the photon emission from the brain slices originates from the energy metabolism of the inner mitochondrial respiratory chain through the production of reactive oxygen. Imaging of ultraweak photon emission from a brain constitutes a novel method, with the potential to extract pathophysiological information associated with neural metabolism and oxidative dysfunction of the neural cells.
doi_str_mv 10.1016/S0168-0102(99)00040-1
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The first demonstration of two-dimensional in vivo imaging of ultraweak photon emission from a rat’s brain, using a highly sensitive photon counting apparatus, is reported in this paper. It was found that the emission intensity correlates with the electroencephalographic activity that was measured on the cortical surface and this intensity is associated with the cerebral blood flow and hyperoxia. To clarify the mechanism of photon emission, intensity changes from whole brain slices were examined under various conditions. The removal of glucose from the incubation medium suppressed the photon emission, and adding 50 mM potassium ions led to temporal enhancement of emission and subsequent depression. Rotenone (20 μM), an inhibitor of the mitochondrial electron transport chain, increased photon emission, indicating electron leakage from the respiratory chain. These results suggest that the photon emission from the brain slices originates from the energy metabolism of the inner mitochondrial respiratory chain through the production of reactive oxygen. 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1872-8111
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Animals
Biophoton
Brain - metabolism
Brain - physiology
Brain Mapping
Cerebral metabolism
Chemiluminescence
Electroencephalography
Imaging
Luminescent Measurements
Neurons - metabolism
Oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - physiology
Photons
Rats
Rats, Wistar
Reactive oxygen
Time Factors
title In vivo imaging of spontaneous ultraweak photon emission from a rat’s brain correlated with cerebral energy metabolism and oxidative stress
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